FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_2187_day_3_ll-e.3510000008bbed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_2187_day_3_ll-e.3510000008bbed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258191
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA34311.328861191908316No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG28361.0984116409944575No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT24970.9671134934989988No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC14130.5472692696492132No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG13670.5294530018474696No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT12180.4717437865766041No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA11500.4454066950435918No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC10870.421006154358595No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT10740.41597112215375437No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA10680.4136472611361357No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA10550.40861222893129506No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC9510.3683319712925702No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG9390.3636842492573328No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT9070.35129032383003284No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG8400.32534054246662353No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT8150.31565778822654544No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA7760.3005526916120237No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG6970.26995518821337694No Hit
TCATCCTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGATCT6830.26453284583893316No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA6650.25756126278607694No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT6590.2552374017684582No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA6520.25252623058123636No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC6440.2494277492244114No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA6390.24749119837639577No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA6300.24400540684996766No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA6260.24245616617155516No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT6220.2409069254931427No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG6080.23548458311869894No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG5970.2312241712530646No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT5970.2312241712530646No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC5910.22890031023544585No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG5910.22890031023544585No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC5790.22425258820020838No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG5770.2234779678610021No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT5760.22309065769139902No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG5760.22309065769139902No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC5640.21844293565616152No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC5610.21728100514735216No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC5530.21418252379052719No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC5390.20876018141608343No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT5290.2048870797200522No Hit
GGAACACACATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACA4950.19171853395354602No Hit
CCCCTGTCCTGAGAGGATTTCTCATTTTGGGCAAAGAAGACAAGAGATAT4760.18435964073108668No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT4760.18435964073108668No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGT4340.1680926136077555No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA4320.16731799326854926No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT4310.16693068309894613No Hit
GTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA4300.16654337292934301No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG4240.16421951191172426No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG4150.16073372038529615No Hit
CTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGA4100.15879716953728054No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA4070.15763523902847118No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC4060.15724792885886804No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT4050.15686061868926493No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG4040.1564733085196618No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT3980.15414944750204307No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT3930.15221289665402746No Hit
AAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAATGTTAAATACATA3930.15221289665402746No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG3920.15182558648442432No Hit
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG3890.15066365597561493No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA3860.1495017254668056No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG3840.14872710512759932No Hit
GTTCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTG3790.1467905542795837No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3760.14562862377077435No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT3740.1448540034315681No Hit
CTCTATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCC3690.14291745258355248No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT3640.14098090173553685No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG3630.14059359156593376No Hit
CATCATCCTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATCTTCATGAT3580.13865704071791815No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC3530.1367204898699025No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG3470.1343966288522838No Hit
CATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACC3430.1328473881738713No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA3400.1316854576650619No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3370.13052352715625254No Hit
GTATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG3340.12936159664744318No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC3330.12897428647784007No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC3280.12703773562982443No Hit
CTATAACACGTGCATGGAAAGTGTCAAAAATGGGACTTATGACTACCCAA3250.12587580512101507No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA3240.12548849495141193No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT3180.12316463393379319No Hit
GCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGTAG3110.12045346274657134No Hit
ATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTC3110.12045346274657134No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTTTC3080.11929153223776197No Hit
GGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAG3070.11890422206815884No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC3070.11890422206815884No Hit
GTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAATGTTAAATA3060.1185169118985557No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC3020.11696767122014323No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAAATGTCACG3000.11619305088093698No Hit
GGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTC2980.11541843054173073No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTAG2970.11503112037212762No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG2960.11464381020252448No Hit
GGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGA2930.11348187969371512No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG2920.11309456952411201No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC2890.11193263901530263No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2820.10922146782808076No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA2820.10922146782808076No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGT2810.10883415765847763No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATT2760.10689760681046201No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT2730.10573567630165265No Hit
GTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGTTCTTTTCCATGA2720.10534836613204954No Hit
GGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGA2710.1049610559624464No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC2700.10457374579284327No Hit
GTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTT2700.10457374579284327No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT2700.10457374579284327No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC2700.10457374579284327No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTATAACA2700.10457374579284327No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT2660.10302450511443079No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT2650.10263719494482766No Hit
GGATCTTCAGTCAATGCACCTGCATCCTTTCCAAGTCATTCTGAGTGCGG2610.10108795426641518No Hit
ATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGCCC2610.10108795426641518No Hit
ATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATT2610.10108795426641518No Hit
TACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTG2610.10108795426641518No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG2600.10070064409681205No Hit
CCCCCAGGGAGACTACCAGTACCAATGAACTGGCGACAGTTGAATAGATC2590.10031333392720891No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTACC100.007087837145.02
TACGCTA100.007087837145.02
GGGGTAC100.007087837145.01
CGCTAGA100.007087837145.04
ACGCTAG100.007087837145.03
GTACGCT100.007087837145.01
CTTACGA100.007087837145.01
GAGCGAA3100.0137.983871
GAGCAAA14150.0134.240281
TATGGTG203.862773E-4108.755
CTAATAA301.5972013E-596.6666649
AGCAGGC4200.093.214298
ACTAATA406.1045466E-790.6258
AAGCAGG25850.090.0290157
TTCTATG259.378804E-487.02
AGCAGGT12500.086.428
GCAGGTC7400.085.236489
GCAGGTA5200.085.048089
GCAGGGT6650.082.857159
GCAGGCA4700.081.755329
Produced by FastQC (version 0.11.2)