FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_2015_day_8_ll-e.3510000008bbfa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_2015_day_8_ll-e.3510000008bbfa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336408
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA17030.5062305295950156No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC16250.4830443984685263No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA11280.33530712705999854No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA8430.2505885710209032No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA7560.2247271170721267No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA6210.18459727473781837No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT6120.18192195191553115No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT5460.16230291788542484No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA4760.14149485148985755No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG4610.13703598011937884No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT4560.1355496896625526No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA4400.13079356020070868No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA4380.1301990440179782No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC4350.12930726974388243No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT4350.12930726974388243No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4240.12603743073886473No Hit
GAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTA3870.11503888135835057No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG3830.11384984899288958No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC3830.11384984899288958No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA3770.1120663004446981No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA3630.10790468716558464No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA3610.10731017098285416No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC3560.10582388052602792No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC3450.1025540415210102No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG3450.1025540415210102No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3410.10136500915554922No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA3370.10017597679008823No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTG100.007088785145.02
AGGTACG203.8638024E-4108.756
GAGCGAA4400.0105.4545441
TACGGTA259.381295E-487.09
GCGAAGC353.4339555E-582.857143
AGCAAAA20400.079.963242
GCAAAAG21250.076.764713
GAGCAAA21700.075.506911
AAAAGCA23000.070.923915
CAAAAGC24150.068.1469964
AAAGCAG30450.067.857146
AGCGAAA6600.067.0075761
AAGGTCG554.0557516E-665.909099
GCGAAAG7050.062.73052
GGTACGG350.003564447462.1428577
CGAAAGC7400.059.7635153
CGAAGCA553.2159273E-452.7272724
AAGCAGG40900.051.0513467
GCAGGGG11700.047.0940179
GGTATTC2400.045.3125145
Produced by FastQC (version 0.11.2)