FastQCFastQC Report
Mon 24 Apr 2017
000000000-AYAYN_l01n01_2001_day_1_ll-e.3510000008bbd0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AYAYN_l01n01_2001_day_1_ll-e.3510000008bbd0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325932
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA20300.622829301817557No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC17580.5393763116232834No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA9360.2871764662567652No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA7920.2429954714480321No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA7580.2325638476737479No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA6360.19513272707190457No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC5390.16537191806879964No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA5390.16537191806879964No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA4820.1478836076236761No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT4790.1469631702318275No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT4740.1454291079120798No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA4680.1435882331283826No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT4650.14266779573653401No Hit
CCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGA4230.1297816722506535No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4110.12609992268325906No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA4060.1245658603635114No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA4050.12425904789956188No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC3880.11904323601241978No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG3730.11444104905317673No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA3720.11413423658922721No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA3670.11260017426947952No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT3540.10861161223813555No Hit
GAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTA3440.1055434875986402No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG3410.1046230502067916No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA3320.10186173803124578No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGCGT404.80577E-9108.749992
GAGCGAA3300.0103.2575761
AGAACCG301.5978338E-596.6666645
AGCAAAA19800.080.9217152
ACCGAGT451.2310156E-680.555558
GCGTGAG554.3850378E-879.090915
GCAAAAG20300.078.9285663
CAAAAGC21950.073.656044
GAGCAAA22250.073.3146061
CCGAGTC502.3027478E-672.59
AAAAGCA24250.066.6701055
AAAGCAG29650.065.5312046
AGCGTGA702.3296161E-762.1428574
ATCCACG350.00356434762.1428571
ATACCGA350.00356434762.1428576
AGCGAAA5300.061.5566022
GCGAAAG5400.060.4166683
CGAAAGC5700.057.2368433
GAAAGCG805.861366E-754.3749961
TCCACGA400.006047242354.3749962
Produced by FastQC (version 0.11.2)