FastQCFastQC Report
Mon 3 Apr 2017
000000000-AY9TV_l01n01_sample8.35100000084e77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY9TV_l01n01_sample8.35100000084e77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1799016
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGTTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCC50562828.105808953338933No Hit
AGGCTGTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAAAAAAC32698918.175991764386755No Hit
AGGCTGGGATTTCCTTTGCCATTCAGCATTGACAAGTAGTCTGTTCAAAA1013865.635636370104546No Hit
AGGCTGTGCTTCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG892224.9594889650786875TruSeq Adapter, Index 6 (96% over 25bp)
AGGCTGTTAATGATCCCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCT581503.232322558554232No Hit
AGGCTGTTAATGATCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTC576363.203751384089969No Hit
AGGCTGTTAATGATCCCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCT526532.926766632425726No Hit
AGGCTGTGCTTCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTG392092.179469220951898TruSeq Adapter, Index 9 (95% over 23bp)
AGGCTGTGCTTCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGC371842.066907687313509RNA PCR Primer, Index 23 (96% over 25bp)
AGGCTGTTAATGATCCCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCT361672.0103767837528963No Hit
AGGCTGGTCTCCCTGGGGGCAATCATCCACTGTAGATCTCGTATGCCGTC255741.4215548944534122No Hit
AGGCTGGTCTCCCTGGGGGCAATCATCCAGAACGCATCTCGTATGCCGTC255221.4186644254414635No Hit
AGGCTGTGCTTCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCT202491.1255597504413524No Hit
AGGCTGGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTG181351.0080510679171282No Hit
AGGCTGTTAATGATCCCTGGGTTTCTACTGAACGCATCTCGTATGCCGTC178970.9948216135932088No Hit
AGGCTGTTAATGATCCCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTC150840.8384583572352886No Hit
AGGCTGGGATTTCCTTTGCCATGAGTAATGAAGGATCTTATTTCTTCGGA144710.8043841744598159No Hit
AGGCTGGTCTCCCTGGGGGCAATCATCCATCCACTATCTCGTATGCCGTC141140.7845399929739368No Hit
AGGCTGTGCTTCTGGGTTTCTACTGAACGCATCTCGTATGCCGTCTTCTG132280.7352908478857331Illumina PCR Primer Index 3 (95% over 24bp)
AGGCTGTTAATGATCCCTGGGTTTTACTGAACGCATCTCGTATGCCGTCT127620.7093877986632693No Hit
AGGCTGGGTGCTTTTATGTGGAGTTGCCATTCAGCATTGACAAGTAGTCT112230.6238410330980936No Hit
AGGCTGTGCTTCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCTTCTGC98460.5472991902239891RNA PCR Primer, Index 23 (96% over 25bp)
AGGCTGTTAATGATCCCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCT96690.5374604783948559No Hit
AGGCTGTGCTTCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCTTCTGC49170.27331607945676967RNA PCR Primer, Index 23 (96% over 25bp)
AGGCTGTGCTTCTGGGTTGCATTAATCAGGGGACGACCTAAAGAAAAAAC48630.270314438559746No Hit
AGGCTGTGCTTCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTGC47840.2659231490992854TruSeq Adapter, Index 6 (96% over 26bp)
AGGCTGGTCTCCCTGGGGGCCATCATCCAGAACGCATCTCGTATGCCGTC37020.2057791592737363No Hit
AGGCTGTTAATGATCCCTGGGTTTTGCTCTGTAGATATCGTATGCCGTCT32920.18298892283337112No Hit
AGGCTTTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT31400.17453985956767476No Hit
AGGCTGTTAATGATCCCTGGGTTTTACTCTGTAGATATCGTATGCCGTCT30320.16853657777362738No Hit
AGGCTGGGATTTCCTTTGCCATCTCTTCCGATCTAGGCTGTTAATGATCC29900.16620196818705335No Hit
AGGCTGTTAATGATCCCTGGGTTTTACTTCCACTATCTCGTATGCCGTCT28800.160087514507931RNA PCR Primer, Index 6 (95% over 21bp)
AGGCTGGGTGCTTTTATGTGGAGTTGGTAATGAAACGGAAACGGGACTCT27370.15213872472507195No Hit
AGGCTGTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT26850.1492482557131232No Hit
AGGCTGTTAATGATCCCTGGGTTTTGCTTCCACTATCTCGTATGCCGTCT24820.13796430937801554RNA PCR Primer, Index 6 (95% over 21bp)
AGGCTGTTAATGATCCCTTGTTTCTACTGAACGCATCTCGTATGCCGTCT24580.1366302467571161No Hit
AGGCTGGTCTCCCTGGGGGCAATCAGTTTCTGGGCCATCATCCAGAACGC24100.13396212151531728No Hit
AGGCTGTTAATGATCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT23320.12962641799739413No Hit
AGGCTGGTCTCCCTGGGGGCCATCATCCATCCACTATCTCGTATGCCGTC22560.12540188636454597No Hit
AGGCTGTGCTTCTGGGTTGAATTCATCAGGGGACGACCTAAAGAAAAAAC21570.11989887805333582No Hit
AGGCTGTGCTTCTGGGTTCAACTCCTTCCTTACACATGCATTGAGTTAGT21010.11678606527123717No Hit
AGGCTGTTATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT20410.11345090871898861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCT700.0294.998966
TGGGCGC750.0294.998965
GGCTGTG610650.0294.56422
GCTGTGC608550.0294.344543
GGCTGGT95550.0294.072752
TGTGCTT609200.0293.8615
GTGCTTC609150.0293.764076
CTGTGCT610100.0293.572574
GCTGGTC95000.0293.13583
GCTGGGA161650.0292.90033
GGCTGGG181800.0292.88952
AGGCTGG278600.0292.82831
AGGCTGT1510400.0292.645481
GGGATTT160150.0291.959666
CTTCTGG613050.0291.943369
GGTCTCC94850.0291.88886
TGGTCTC94950.0291.736725
CTGGGAT162050.0291.722234
GGGTGCT17400.0291.60826
TGGGATT162450.0291.45795
Produced by FastQC (version 0.11.2)