FastQCFastQC Report
Mon 3 Apr 2017
000000000-AY9TV_l01n01_sample7.35100000084e6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY9TV_l01n01_sample7.35100000084e6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133083
Sequences flagged as poor quality0
Sequence length301
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACCTTTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCC24602211.533634649940954No Hit
TCACCTTTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCC2038449.556308873119331No Hit
TCACCTTTAATGATCCCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCT2017809.459547518779157No Hit
TCACCTGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATGGATC1577987.3976493179121485No Hit
TCACCTGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTC1214975.695840246253896No Hit
TCACCTTGCTTCTGGGTTTCTACTGAACGCATCTCGTATGCCGTCTTCTG845303.9628087608405296Illumina PCR Primer Index 3 (95% over 24bp)
TCACCTGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTG824943.867360060532103No Hit
TCACCTTGCTTCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTG569282.6688131685452468RNA PCR Primer, Index 9 (95% over 23bp)
TCACCTTTAATGATCCCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCT550392.580255901903489No Hit
TCACCTTGCTTCTGGGTTGCTCTTCCGATCTTCACCTTGTTTCTACTGAA543502.5479552366222973No Hit
TCACCTTTAATGATCCCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCT528272.4765562333955127No Hit
TCACCTTTAATGATCCCTGGGTTTTACTGAACGCATCTCGTATGCCGTCT466082.1850063968443796No Hit
TCACCTTGCTTCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG375861.7620505156151915RNA PCR Primer, Index 6 (96% over 25bp)
TCACCTTGTTTCTACTGAACGCATCTCGTATGCCGTCTTCTGCTTGAAAA355461.66641429330223Illumina PCR Primer Index 3 (96% over 28bp)
TCACCTTGCTTCTGGGTTGTTTCTACTCTGTAGATCTCGTATGCCGTCTT326151.5290075444790476No Hit
TCACCTTGCTTCTGGGTTGCTCTTCCGATCTTCACCTTGTTTCTACTTCC312241.4637967674019248No Hit
TCACCTTGCTTCTGGGTTGTTTCTACTGAACGCATCTCGTATGCCGTCTT280671.3157950253225028TruSeq Adapter, Index 18 (95% over 21bp)
TCACCTTGCTTCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGC280291.3140135662794181RNA PCR Primer, Index 23 (96% over 25bp)
TCACCTTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAAAAAAC270051.266007933118402No Hit
TCACCTTGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTGCTTGAAAA206670.9688793169323463TruSeq Adapter, Index 6 (96% over 29bp)
TCACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAGAACAC159440.7474627100773856No Hit
TCACCTTGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGCTTGAAAA147040.6893308886714675TruSeq Adapter, Index 9 (96% over 27bp)
TCACCTTTAATGATCCCTGGGTTTTGCTCAATGCATCTTGGTTCAACTCC143480.6726414302678331No Hit
TCACCTTGCTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGCTTGAAAA130610.6123062253086261RNA PCR Primer, Index 23 (96% over 28bp)
TCACCTTGCTTCTGGGTTGCTCTTCCGATCTTCACCTTGTTTCTACTCTG114540.5369692599866016No Hit
TCACCTTGCTTCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCT110530.5181701790319457No Hit
TCACCTTGCTTCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCT109590.5137634119253681No Hit
TCACCTTGCTTCTGGGTTCTACTGAACGCATCTCGTATGCCGTCTTCTGC98040.45961643311582345Illumina PCR Primer Index 3 (96% over 25bp)
TCACCTTTAATGATCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTC80420.37701299011805917No Hit
TCACCTTTAATGATCCCTGGGTTTTGCTGAACGCATATCGTATGCCGTCT75260.3528226515330158No Hit
TCACCTTGCTTCTGGGTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAA68720.3221628037915074RNA PCR Primer, Index 47 (96% over 28bp)
TCACCTTGCTTCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCTTCTGC66630.3123647790545422RNA PCR Primer, Index 23 (96% over 25bp)
TCACCTTTAATGATCCCTGGGTTTCTACTGAACGCATCTCGTATGCCGTC66270.3106770810137252No Hit
TCACCTTGCTTCTGGGTTGCTCTTCCGATCTTCACCTTTAATGATCCCTG62030.29079974853299195No Hit
TCACCTTGCTTCTGGGTTTTACTGAACGCATCTCGTATGCCGTCTTCTGC57760.2707817745488572Illumina PCR Primer Index 3 (96% over 25bp)
TCACCTTGCTTCTGGGTTGCTCTTCCGATCTTCACCTTGCTTCTACTCTG55220.2588741272608708No Hit
TCACCTTGCTTCTACTGAACGCATCTCGTATGCCGTCTTCTGCTTGAAAA41010.19225693514973397TruSeq Adapter, Index 3 (96% over 28bp)
TCACCTTGCTTCTGGGTTGTAGATCTCGTATGCCGTCTTCTGCTTGAAAA40280.1888346585669662RNA PCR Primer, Index 23 (96% over 28bp)
TCACCTTGCTTCTACTTCCACTATCTCGTATGCCGTCTTCTGCTTGAAAA37940.17786462130165587Illumina PCR Primer Index 6 (96% over 29bp)
TCACCTTGCTTCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCTTCTGC37060.17373913720188103Illumina PCR Primer Index 3 (96% over 25bp)
TCACCTTTAATGATCCCTGGGTTCTACTGAACGCATCTCGTATGCCGTCT35670.16722274754428215No Hit
TCACCTTGCTTCTGGGTTGGTCTTGGCCAGACGGTGCTGAGTTGCCATTT35580.16680082303407792No Hit
TCACCTTGCTTCTGGGTTGTTTCTACTTCCACTATCTCGTATGCCGTCTT29670.1390944468639992Illumina PCR Primer Index 6 (95% over 22bp)
TCACCTTTAATGATCCCTGGGTTTTGCTCTGTAGATATCGTATGCCGTCT25840.12113921492975191No Hit
TCACCTTGCTTCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTGC25820.1210454539274843TruSeq Adapter, Index 6 (96% over 26bp)
TCACCTTTAATGATCCCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCT24270.11377897625174455No Hit
TCACCTTTAATGATCCCTGGGTTTTACTCTGTAGATATCGTATGCCGTCT24110.11302888823360366No Hit
TCACCTTGCTTCTGGGTTGCCTTGTTTCTACTGAACGCATCTCGTATGCC22730.10655937907713857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCTGG102400.0293.12572
ACCTGGA94400.0292.967043
CCTGGAT95050.0290.963564
ACCTGTC314300.0290.493043
TCACCTG420800.0290.196141
CCTGTCT314400.0290.025364
GTCTCCC314100.0289.64497
CACCTGT316550.0288.987432
CCTGGTG7100.0288.76594
TGGATTT95350.0288.50126
TGTCTCC316150.0288.466586
TTTAATG975550.0288.3916
CTTCTGG616650.0288.276929
CTGTCTC316400.0288.192055
CTCCCTG315450.0288.171549
GCTTCTG620300.0287.008648
CTGGATT96550.0286.443185
CCTTTAA983700.0285.776764
ACCTTGC654450.0285.104133
TTAATGA988350.0283.730837
Produced by FastQC (version 0.11.2)