FastQCFastQC Report
Mon 3 Apr 2017
000000000-AY9TV_l01n01_sample6.35100000084e5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY9TV_l01n01_sample6.35100000084e5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1266345
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGCTTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCC38524030.42140964744994No Hit
GTCAGCTGCTTCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG16637913.13852070328386TruSeq Adapter, Index 6 (96% over 25bp)
GTCAGCGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATGGATC1091028.615503673959308No Hit
GTCAGCTTAATGATCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTC1030188.135065878571796No Hit
GTCAGCGTCTCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCT420333.3192376485081083RNA PCR Primer, Index 6 (95% over 24bp)
GTCAGCGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTG275372.174525899340227No Hit
GTCAGCTGCTTCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCT260372.0560747663551404No Hit
GTCAGCTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAAAAAAC250861.980976748042595No Hit
GTCAGCGGTGCTTTTATGTGGAGTTGCCATTCAGCATTGACAAGTAGTCT235661.8609462666177068No Hit
GTCAGCTGCTTCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTGC201521.591351487943649RNA PCR Primer, Index 6 (96% over 26bp)
GTCAGCTGCTTCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTG188801.490904927172295TruSeq Adapter, Index 9 (95% over 23bp)
GTCAGCTTAATGATCCCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCT145141.146133162763702No Hit
GTCAGCTGCTTCTGGGTTTCTACTGAACGCATCTCGTATGCCGTCTTCTG134651.063296337096131Illumina PCR Primer Index 3 (95% over 24bp)
GTCAGCTTAATGATCCCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCT125910.9942788102768203No Hit
GTCAGCTGCTTCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGC123010.9713782578997034RNA PCR Primer, Index 9 (95% over 24bp)
GTCAGCTTAATGATCCCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCT120740.9534526531079603No Hit
GTCAGCTTAATGATCCCTGGGTTTCTACTGAACGCATCTCGTATGCCGTC112730.8901997480939238No Hit
GTCAGCTTAATGATCCCTGGGTTTTACTTCCACTATCTCGTATGCCGTCT79870.6307127994345932No Hit
GTCAGCTTAATGATCCCTGGGTTTTACTGAACGCATCTCGTATGCCGTCT74150.5855434340562801No Hit
GTCAGCTTAATGATCCCTGGGTTCTACTTCCACTATCTCGTATGCCGTCT68660.5421903193837383No Hit
GTCAGCTGCTTCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCTTCTGC68460.5406109709439371RNA PCR Primer, Index 9 (95% over 24bp)
GTCAGCGGTGCTTTTATGTGGAGTTGATAAGGGGAAGAAAAGAGGAAACT45170.35669584512909197No Hit
GTCAGCGGATTTCCTTTGCCATTCAGCATTGACAAGTAGTCTGTTCAAAA42380.3346639343938658No Hit
GTCAGCTTAATGATCCCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTC41060.32424023469117813No Hit
GTCAGCGTCTCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCC40460.31950218937177466No Hit
GTCAGCGTCTCCCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTG37900.29928652934231986TruSeq Adapter, Index 6 (96% over 25bp)
GTCAGCGTCTCCCTGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTA30600.2416403112895775No Hit
GTCAGCTGCTTCTGGGTTTTACTTCCACTATCTCGTATGCCGTCTTCTGC28260.22316193454390393RNA PCR Primer, Index 6 (96% over 26bp)
GTCAGCAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTG27390.21629176883076884No Hit
GTCAGCTGCTTCTGGGTTCTACTGAACGCATCTCGTATGCCGTCTTCTGC25840.20405181842230988Illumina PCR Primer Index 3 (96% over 25bp)
GTCAGCTTAATGATCCCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCT24490.19339121645365207No Hit
GTCAGCTTAATGATCCCTGGGTTTTGCTTCCACTATCTCGTATGCCGTCT19990.15785587655812594No Hit
GTCAGCTGCTTCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATGGATCT19370.15295989639474233No Hit
GTCAGCAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAAT19290.15232815701882188No Hit
GTCAGCTTAATGATCCCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATG18180.14356277317792546No Hit
GTCAGCAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTG17730.14000923918837282No Hit
GTCAGCTGCTTCTGGGTTTTACTGAACGCATCTCGTATGCCGTCTTCTGC16390.12942760464170505Illumina PCR Primer Index 3 (96% over 25bp)
GTCAGCTTAATGATCCCTGGGTTTCTACTTCCACTATATCGTATGCCGTC16340.12903276753175477No Hit
GTCAGCTGCTTCTGGGTTTCTACTTCCACTACCTCGTATGCCGTCTTCTG15350.12121499275473903Illumina PCR Primer Index 6 (95% over 21bp)
GTCAGCAGCGAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAAT15000.118451132985087No Hit
GTCAGCTGCTTCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCTTCTGC14990.11837216556309695RNA PCR Primer, Index 9 (95% over 24bp)
GTCAGCTGCTTCTGGGCTTCTACTTCCACTATCTCGTATGCCGTCTTCTG14380.11355515282170339Illumina PCR Primer Index 6 (96% over 25bp)
GTCAGCTTAATGATCCCTGGGTTCTACTGAACGCATCTCGTATGCCGTCT13500.10660601968657829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGGG108.4654463E-4294.99634
GCGGGTT108.4654463E-4294.99635
GCGGGGC108.4654463E-4294.99635
GTCAGCT981250.0294.72571
CAGCTGC342750.0294.221683
GTCAGCG255300.0294.129671
TCAGCTG344200.0294.096372
AGCGTCT183950.0293.873724
AGCTGCT342850.0293.834724
GCGTCTC183850.0293.712655
AGCGGTG33200.0293.663484
GCTGCTT342900.0293.318735
CAGCGTC184750.0293.160033
CAGCTTA639800.0292.967563
GCGGATT35850.0292.939125
CTTCTGG344200.0292.4689
TCAGCTT641000.0292.373082
GCGGTGC33150.0292.326635
GCTTCTG344400.0292.21258
CAGCGGA36050.0292.132263
Produced by FastQC (version 0.11.2)