FastQCFastQC Report
Mon 3 Apr 2017
000000000-AY9TV_l01n01_sample5.35100000084e41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY9TV_l01n01_sample5.35100000084e41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences894859
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCC31985835.743955192941016No Hit
TACGGAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATGGATC12302013.747417190864706No Hit
TACGGATGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAAAAAAC691977.732726608326004No Hit
TACGGAGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTG625186.986352039818564No Hit
TACGGATGCTTCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG563706.299316428621715TruSeq Adapter, Index 6 (96% over 25bp)
TACGGATGCTTCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCT318693.5613431836747464No Hit
TACGGATGCTTCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTG177171.9798649843159648RNA PCR Primer, Index 23 (95% over 24bp)
TACGGAGGTGCTTTTATGTGGAGTTGATAAGGGGAAGAAAAGAGGAAACT175951.9662315515628719No Hit
TACGGAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTC156011.7434031506639593No Hit
TACGGATGCTTCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGC147031.6430521456452918RNA PCR Primer, Index 9 (95% over 24bp)
TACGGATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCC119621.3367469064958837No Hit
TACGGATTAATGATCCCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCT79340.8866201267462247No Hit
TACGGATTAATGATCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTC67200.7509562959080704No Hit
TACGGATGCTTCTGGGTTCAACTCCTTCCTTACACATGCATTGAGTTAGT60200.6727316817509797No Hit
TACGGAGGTGCTTTTATGTGGAGTTGCCATTCAGCATTGACAAGTAGTCT45830.5121477238313522No Hit
TACGGATTAATGATCCCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCT41960.46890068714736066No Hit
TACGGAGGTGCTTTTATGTGGAGTTGGTAATGAAACGGAAACGGGACTCT39090.43682859534295343No Hit
TACGGATGCTTCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTGC38470.42990012951761114RNA PCR Primer, Index 6 (96% over 26bp)
TACGGATTAATGATCCCTGGGTTTTGCTCAATGCATCTTGGTTCAACTCC37970.42431265707781896No Hit
TACGGATGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAGAACAC28250.3156921928482588No Hit
TACGGATTAATGATCCCTGGGTTTTGCTTCCACTATCTCGTATGCCGTCT20030.22383414593807519TruSeq Adapter, Index 6 (95% over 21bp)
TACGGATTAATGATCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT18860.21075946042896143No Hit
TACGGTTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT18600.20785397476026948No Hit
TACGGAGTCTCCCTGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTA18430.20595423413074013No Hit
TACGGATAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT18260.20405449350121083No Hit
TACGGATGCTTCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCTTCTGC17800.19891401885660198RNA PCR Primer, Index 9 (95% over 24bp)
TACGGATTATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT17280.1931030475192181No Hit
TACGGATTAATGATCCCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCT17070.1907563090945054No Hit
TACGGAGGATTTCCTTTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAA13840.15466123713344784Illumina PCR Primer Index 6 (96% over 28bp)
TACGGATTAATGATCCCTGGTTTTGCTTAATGCTTCTTGGTTCAACTCCT13510.15097350532318501No Hit
TACGGATTAATGATCCCTGGGTTTTACTGAACGCATCTCGTATGCCGTCT12280.13722832312129618No Hit
TACGGATTAATGATCCCTGGGTTTTACTTCCACTATCTCGTATGCCGTCT11850.13242309682307493TruSeq Adapter, Index 6 (95% over 21bp)
TACGGATGCTTCTGGGTTGCATTAATCAGGGGACGACCTAAAGAAAAAAC10890.12169514973867392No Hit
TACGGATGCTTCTGGGTTGAACTCCTTCCTTACACATGCATTGAGTTAGT10710.11968365966034873No Hit
TACGGATGCTTCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCTTCTGC9020.10079800281385112RNA PCR Primer, Index 9 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGTC157.239827E-6295.04
TACGGGT550.0295.01
TACGGGA255.311449E-10295.01
ACGGGAG157.239827E-6295.02
CGGGAGT157.239827E-6295.03
CGGAGGT29200.0295.03
GGAGGTG28850.0295.04
CGGGTGC108.464715E-4294.999973
GGTGTCT108.464715E-4294.999974
CTCCCGG108.464715E-4294.999979
ACGGGAT108.464715E-4294.999972
CCGAGGC206.1842E-8294.999973
CGGTGTC108.464715E-4294.999973
GAGGGGC108.464715E-4294.999975
CGGAGGG206.1842E-8294.999973
TCGCGAC108.464715E-4294.99997295
TACGGAG254750.0294.76841
CGGATGC231950.0294.745643
ACGGAGG102600.0294.712462
CGGAGTC150200.0294.705383
Produced by FastQC (version 0.11.2)