FastQCFastQC Report
Mon 3 Apr 2017
000000000-AY9TV_l01n01_sample4.35100000084e34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY9TV_l01n01_sample4.35100000084e34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939808
Sequences flagged as poor quality0
Sequence length301
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTACTTAATGATCCCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCT20089110.356231132153285No Hit
CAGTACTTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCC1759929.072650489120573No Hit
CAGTACTTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCC1472357.590184183176891No Hit
CAGTACTGCTTCTGGGTTTCTACTGAACGCATCTCGTATGCCGTCTTCTG1389987.165554529108036Illumina PCR Primer Index 3 (95% over 24bp)
CAGTACTGCTTCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG1361527.0188389778782225TruSeq Adapter, Index 6 (96% over 25bp)
CAGTACTGCTTCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTG1028025.299596661112852TruSeq Adapter, Index 9 (95% over 23bp)
CAGTACGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATGGATC994755.1280848413863644No Hit
CAGTACGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTC800244.1253567363367925No Hit
CAGTACTGCTTCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGC726833.746917220673386RNA PCR Primer, Index 9 (95% over 24bp)
CAGTACTTAATGATCCCTGGGTTTTGCTCAATGCATCTTGGTTCAACTCC678833.499470050644187No Hit
CAGTACGGTGCTTTTATGTGGAGTTGCCATTCAGCATTGACAAGTAGTCT541172.789812187597948No Hit
CAGTACGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTG522652.694338821161682No Hit
CAGTACTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAAAAAAC466462.404670977746251No Hit
CAGTACTTAATGATCCCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCT404192.0836598261271218No Hit
CAGTACTTAATGATCCCTGGGTTTTACTGAACGCATCTCGTATGCCGTCT388232.001383642092413No Hit
CAGTACGGTGCTTTTATGTGGAGTTGATAAGGGGAAGAAAAGAGGAAACT362281.8676075157953775No Hit
CAGTACTTAATGATCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTC247581.2763118824131048No Hit
CAGTACTTAATGATCCCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCT221071.1396488724657285No Hit
CAGTACTGCTTCTGGGTTCTACTGAACGCATCTCGTATGCCGTCTTCTGC175290.9036461340503803Illumina PCR Primer Index 3 (96% over 25bp)
CAGTACTGCTTCTGGGTTTTGCTCAATGCATCTTGGTTCAACTCCTTCCT157030.8095131064517724No Hit
CAGTACTGCTTCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCT121700.6273816790115311No Hit
CAGTACTGCTTCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTGC117770.6071219419653904RNA PCR Primer, Index 6 (96% over 26bp)
CAGTACTGCTTCTGGGTTTTACTGAACGCATCTCGTATGCCGTCTTCTGC116840.6023276530460747Illumina PCR Primer Index 3 (96% over 25bp)
CAGTACTGCTTCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCTTCTGC112550.5802120622247151RNA PCR Primer, Index 9 (95% over 24bp)
CAGTACTGCTTCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCTTCTGC81150.4183403718306142Illumina PCR Primer Index 3 (96% over 25bp)
CAGTACGGTGCTTTTATGTGGAGTTGCCATTTACCATTGACAAGTAATTT76620.3949875451591085No Hit
CAGTACTTAATGATCCCTGGGTTTTGCTGAACGCATATCGTATGCCGTCT65040.33529091538956435No Hit
CAGTACTGCTTCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCT60410.31142257378049787No Hit
CAGTACTTAATGATCCCTGGGTTTTGCTTCCACTATCTCGTATGCCGTCT59090.3046177766046949TruSeq Adapter, Index 6 (95% over 21bp)
CAGTACTTAATGATCCCTGGGTTTCTACTGAACGCATCTCGTATGCCGTC52980.27311981391972817No Hit
CAGTACTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAGAACAC48800.25157128952968544No Hit
CAGTACTTAATGATCCCTGGGTTTTACTTCCACTATCTCGTATGCCGTCT45640.23528101750276315TruSeq Adapter, Index 6 (95% over 21bp)
CAGTACAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG38670.19934962635477327No Hit
CAGTACGTCTCCCTGGGGGCAATCTGTAGATCTCGTATGCCGTCTTCTGC35810.1846058991405335RNA PCR Primer, Index 23 (96% over 25bp)
CAGTACTGCTTCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCTTCTGC32690.16852183308863558RNA PCR Primer, Index 9 (95% over 24bp)
CAGTACTTAATGATCCCTGGGTTCTACTGAACGCATCTCGTATGCCGTCT25770.1328481994094261No Hit
CAGTACAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG25580.1318687210280605No Hit
CAGTACGTCTCCCTGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTA21100.1087736518253353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTGC676750.0293.972233
AGTACGG179850.0293.192352
AGTACTG680000.0292.979342
CTTCTGG679950.0292.61049
CAGTACG392150.0292.401341
CAGTACT1528900.0292.276031
TACGGTG116000.0292.072084
GTACGTC210600.0291.985023
GTACGGT116850.0291.967133
GCTTCTG681900.0291.90348
GTACTTA844200.0291.69443
AGTACTT847300.0291.201662
ACTGCTT685000.0290.410165
GTGCTTT116600.0289.93668
CTGCTTC686600.0289.690466
AGTACGT214000.0289.482642
TACTGCT687850.0289.142584
CGGATTT60500.0288.90166
ACGTCTC212000.0288.59575
GTACGGA61650.0288.536833
Produced by FastQC (version 0.11.2)