FastQCFastQC Report
Mon 3 Apr 2017
000000000-AY9TV_l01n01_sample3.35100000084e27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY9TV_l01n01_sample3.35100000084e27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1416447
Sequences flagged as poor quality0
Sequence length301
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCC16387611.569511601916627No Hit
GCTTACTTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCC16072311.346912380060814No Hit
GCTTACTTAATGATCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTC1360239.603112576750137No Hit
GCTTACTGCTTCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG1288659.09776362970164TruSeq Adapter, Index 6 (96% over 25bp)
GCTTACGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTC1154108.147851631582403No Hit
GCTTACGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATGGATC1082707.643773469815672No Hit
GCTTACTTAATGATCCCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCT664804.693433640651574No Hit
GCTTACTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAAAAAAC408912.8868711642581757No Hit
GCTTACGTCTCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCT389562.7502617464684525TruSeq Adapter, Index 6 (95% over 24bp)
GCTTACTTAATGATCCCTGGGTTTCTACTAGTGTAATCTCGTATGCCGTC291572.0584603589121233No Hit
GCTTACTGCTTCTGGGTTTCTACTAGTGTAATCTCGTATGCCGTCTTCTG242931.7150659361063278TruSeq Adapter, Index 12 (96% over 25bp)
GCTTACTTAATGATCCCTGGGTTTTGCTTCCACTATCTCGTATGCCGTCT223861.5804332954215725TruSeq Adapter, Index 6 (95% over 21bp)
GCTTACGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTG186681.317945535554807No Hit
GCTTACTTAATGATCCCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCT176891.2488289360632625No Hit
GCTTACTTAATGATCCCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCT158711.120479622605011No Hit
GCTTACTTAATGATCCCTGGGTTTTACTTCCACTATCTCGTATGCCGTCT137750.9725037364617243TruSeq Adapter, Index 6 (95% over 21bp)
GCTTACTGCTTCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTG119370.8427424393570674TruSeq Adapter, Index 9 (95% over 23bp)
GCTTACTTAATGATCCCTGGGTTTTGCTAGTGTAATCTCGTATGCCGTCT110530.7803327621859484TruSeq Adapter, Index 12 (95% over 21bp)
GCTTACTTAATGATCCCTGGGTTTTGCTCAATGCATCTTGGTTCAACTCC108820.7682603020091822No Hit
GCTTACTGCTTCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGC106990.7553406516445726RNA PCR Primer, Index 9 (95% over 24bp)
GCTTACTGCTTCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTGC87220.6157660681973981RNA PCR Primer, Index 6 (96% over 26bp)
GCTTACTTAATGATCCCTGGGTTTTACTAGTGTAATCTCGTATGCCGTCT85240.6017874300979846TruSeq Adapter, Index 12 (95% over 21bp)
GCTTACTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAGAACAC74670.5271640943854589No Hit
GCTTACTGCTTCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCT43520.30724764145781663No Hit
GCTTACGTCTCCCTGGGTTTCTACTAGTGTAATCTCGTATGCCGTCTTCT42950.3032234880655612TruSeq Adapter, Index 12 (95% over 24bp)
GCTTACTTAATGATCCCTGGGTTCTACTTCCACTATCTCGTATGCCGTCT40150.2834557170158855TruSeq Adapter, Index 6 (95% over 21bp)
GCTTACTTAATGATCCCTGGGTTTTCTACTTCCACTATCTCGTATGCCGT33030.23318909920385303No Hit
GCTTACGTCTCCCTGGGTTTTGCTTCCACTATCTCGTATGCCGTCTTCTG32620.23029453272872194TruSeq Adapter, Index 6 (96% over 25bp)
GCTTACTGCTTCTGGGTTCTACTAGTGTAATCTCGTATGCCGTCTTCTGC31290.22090484148012596RNA PCR Primer, Index 12 (96% over 26bp)
GCTTACTTAATGATCCCTGGGTTTTGCTCTGTAGATATCGTATGCCGTCT30630.21624529544698812No Hit
GCTTACTGCTTCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCTTCTGC25640.18101630346917322RNA PCR Primer, Index 9 (95% over 24bp)
GCTTACGTCTCCCTGGGGGCCTCTTCCGATCTACTTCCACTATCTCGTAT25580.1805927083752516No Hit
GCTTACGTCTCCCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTG24840.1753683688835516TruSeq Adapter, Index 6 (96% over 25bp)
GCTTACTTAATGATCCCTGGGTTTCTACTTCCACTATATCGTATGCCGTC19590.13830379816540964No Hit
GCTTACGTCTCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCC18660.1317380742096245No Hit
GCTTACGTCTCCCTGGGGGCACTCTTCCGATCTACTTCCACTATCTCGTA18340.12947890037537585No Hit
GCTTACGTCTCCCTGGGTTTTGCTCAATGCATCTTGGTTCAACTCCTTCC18150.12813751591129072No Hit
GCTTACTTAATGATCCCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCT17240.12171299032014611No Hit
GCTTACGTCTCCCTGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG16350.11542966309364205TruSeq Adapter, Index 6 (96% over 25bp)
GCTTACTTAATGATCCCTGGGTTTTACTGAACGCATCTCGTATGCCGTCT16220.11451187372347853No Hit
GCTTACTGCTTCTGGGTTGGTCTTGGCCAGACGGTGCTGAGTTGCCATTT15750.11119371215442583No Hit
GCTTACGTCTCCCTGGGGGCTTGTTTCTACTTCCACTATCTCGTATGCCG14870.10498098411024204No Hit
GCTTACTGCTTCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCTTCTGC14320.10109802908262716RNA PCR Primer, Index 9 (95% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTACG351200.0293.778781
GCTTACT1053350.0293.204351
ACGTCTC330150.0291.377935
GTCTCCC328850.0291.184237
CTTACGT331700.0290.950162
CTCCCTG328050.0290.815259
CTTCTGG283650.0290.73279
TTACTGC284200.0290.585273
TTACGTC331850.0290.329743
TACGTCT332500.0289.584724
CGTCTCC332550.0289.230746
TTAATGA775700.0289.159157
GCTTCTG285400.0289.053348
AATGATC776700.0288.426069
CTTACGG22900.0287.267552
ACTGCTT288500.0285.69185
TACTGCT290300.0284.32684
CTGCTTC290100.0284.116126
CGGATTT20400.0282.705176
CTTACTG293350.0282.02432
Produced by FastQC (version 0.11.2)