FastQCFastQC Report
Mon 3 Apr 2017
000000000-AY9TV_l01n01_sample2.35100000084e1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY9TV_l01n01_sample2.35100000084e1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1212876
Sequences flagged as poor quality0
Sequence length301
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGTATGCTTCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG17102214.100534597106382Illumina PCR Primer Index 1 (95% over 24bp)
CATGTAGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATGGATC14878412.267041313374163No Hit
CATGTATGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAAAAAAC666655.496439866894884No Hit
CATGTAGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTG629035.186268010909607No Hit
CATGTATGCTTCTGGGTTTCTACTGAACGCATCTCGTATGCCGTCTTCTG579594.7786418397264026Illumina PCR Primer Index 3 (95% over 24bp)
CATGTATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCC562864.640705232851503No Hit
CATGTATTAATGATCCCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCT530164.371098117202418No Hit
CATGTATGCTTCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGC507774.186495569209053RNA PCR Primer, Index 23 (96% over 25bp)
CATGTATGCTTCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTG392823.23874823147626RNA PCR Primer, Index 23 (95% over 24bp)
CATGTATGCTTCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTGC337782.7849508111299093Illumina PCR Primer Index 1 (96% over 25bp)
CATGTAGGTGCTTTTATGTGGAGTTGATAAGGGGAAGAAAAGAGGAAACT287142.367430800840317No Hit
CATGTATTAATGATCCCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCT267752.207562850612923No Hit
CATGTATTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCC237541.9584854511095942No Hit
CATGTAGTCTCCCTGGGGGCAATCTGTAGATCTCGTATGCCGTCTTCTGC235051.9379557349638379Illumina PCR Primer Index 9 (95% over 24bp)
CATGTATTAATGATCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTC199521.6450156487555199No Hit
CATGTATGCTTCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCT194551.6040386651232277No Hit
CATGTATTAATGATCCCTGGGTTTTGCTCAATGCATCTTGGTTCAACTCC156201.2878480570148967No Hit
CATGTATGCTTCTGGGTTTCTACTAGTGTAATCTCGTATGCCGTCTTCTG140001.1542812290786528Illumina PCR Primer Index 12 (100% over 24bp)
CATGTATTAATGATCCCTGGGTTTTGCTTCCACTATCTCGTATGCCGTCT136251.1233629818711888TruSeq Adapter, Index 6 (95% over 21bp)
CATGTATGCTTCTGGGTTCTACTGAACGCATCTCGTATGCCGTCTTCTGC135921.120642176116932Illumina PCR Primer Index 3 (96% over 25bp)
CATGTATGCTTCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCTTCTGC125221.032422110751635RNA PCR Primer, Index 23 (96% over 25bp)
CATGTATTAATGATCCCTGGGTTTTACTTCCACTATCTCGTATGCCGTCT114040.9402445097437826TruSeq Adapter, Index 6 (95% over 21bp)
CATGTAGTCTCCCTGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTA103660.8546628014735224No Hit
CATGTATGCTTCTGGGTTTTGCTCAATGCATCTTGGTTCAACTCCTTCCT93650.7721316935943988No Hit
CATGTATTAATGATCCCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCT81080.6684937289549797No Hit
CATGTATTAATGATCCCTGGGTTTTACTGAACGCATCTCGTATGCCGTCT78220.6449134124180873No Hit
CATGTATGCTTCTGGGTTTTACTGAACGCATCTCGTATGCCGTCTTCTGC62820.5179424772194354Illumina PCR Primer Index 3 (96% over 25bp)
CATGTATGCTTCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCTTCTGC57150.47119408744175006RNA PCR Primer, Index 23 (96% over 25bp)
CATGTATGCTTCTGGGTTTTACTTCCACTATCTCGTATGCCGTCTTCTGC46400.38256177878035347Illumina PCR Primer Index 1 (96% over 25bp)
CATGTATGCTTCTGGGTTGACTCTGTAGATCTCGTATGCCGTCTTCTGCT42940.35403454269026674RNA PCR Primer, Index 23 (96% over 26bp)
CATGTATGCTTCTGGGTTCAACTCCTTCCTTACACATGCATTGAGTTAGT36360.29978332492357007No Hit
CATGTATGCTTCTGGGTTGCTTTGTTGGGGTTCATCATGTGGGCCTGCCA36320.2994535302866905No Hit
CATGTATGCTTCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCTTCTGC35710.29442416207427635Illumina PCR Primer Index 3 (96% over 25bp)
CATGTAGGTGCTTTTATGTGGAGTTGGTAATGAAACGGAAACGGGACTCT31790.2621042876600741No Hit
CATGTAGTCTCCCTGGGGGCAAGGAGACGTGGTGTTGGTAATGAAACGAA29400.24239905810651707No Hit
CATGTATGCTTCTGGGTTCTACTAGTGTAATCTCGTATGCCGTCTTCTGC27590.22747585078771448Illumina PCR Primer Index 12 (100% over 25bp)
CATGTATGCTTCTGGGTTGTTTCTACTCTGTAGATCTCGTATGCCGTCTT25880.21337713006111095No Hit
CATGTATGCTTCTGGGTTGAACTCCTTCCTTACACATGCATTGAGTTAGT23840.19655760358025057No Hit
CATGTATGCTTCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCT17080.14082230994759562No Hit
CATGTATTAATGATCCCTGGGTTTTGCTGAACGCATATCGTATGCCGTCT16790.13843129883021843No Hit
CATGTAGTCTCCCTGGGGGCAATCTCTGTAGATCTCGTATGCCGTCTTCT14540.11988035050574007RNA PCR Primer, Index 23 (95% over 23bp)
CATGTATGCTTCTGGGTTTCTACTTCCACTACCTCGTATGCCGTCTTCTG14420.11889096659510123RNA PCR Primer, Index 27 (95% over 22bp)
CATGTATTAATGATCCCTGGGTTCTACTTCCACTATCTCGTATGCCGTCT14250.11748933938836287TruSeq Adapter, Index 6 (95% over 21bp)
CATGTATGCTTCTGGGCTTCTACTTCCACTATCTCGTATGCCGTCTTCTG14080.1160877121816245Illumina PCR Primer Index 6 (96% over 25bp)
CATGTAGGTGCTTTTATGTGGAGTTGGAGTAAAAAACTACCTTGTTTCTA13680.11278976581282835No Hit
CATGTATTAATGATCCCTGGGTTTTCTACTTCCACTATCTCGTATGCCGT12240.10091715888516221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTGG598900.0293.473029
GCTTCTG599650.0293.08148
GTATGCT598250.0293.076874
TGTATGC599100.0292.685673
ATGTATG600850.0292.2262
CATGTAG323950.0291.99491
TGTAGTC218000.0291.684633
GTCTCCC216700.0290.98417
AGTCTCC218600.0290.884036
CATGTAT884350.0290.79691
ATGTAGG105900.0290.542972
CTCCCTG216850.0289.490429
TATGCTT606350.0288.96725
TAGTCTC220250.0288.303075
GTAGGAT65500.0287.34354
ATGTAGT221950.0287.22462
GTAGGTG39950.0286.138924
TGTATTA284950.0285.21673
TGTAGGA66250.0285.203773
AGGTGCT39900.0285.018836
Produced by FastQC (version 0.11.2)