FastQCFastQC Report
Mon 3 Apr 2017
000000000-AY9TV_l01n01_sample1.35100000084e0d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY9TV_l01n01_sample1.35100000084e0d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835014
Sequences flagged as poor quality0
Sequence length301
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGCCTTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCC31929917.400357708442552No Hit
ATAGCCTTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCC26821314.61640074680629No Hit
ATAGCCGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGTTCTAATGGATC21828611.895604066236007No Hit
ATAGCCGTCTCCCTGGGGGCAATCAGTTTCTGGATGTGCTCTAATGGGTC1340277.303867981388698No Hit
ATAGCCTGCTTCTGGGTTTCTACTGAACGCATCTCGTATGCCGTCTTCTG1246836.794662057074224Illumina PCR Primer Index 3 (95% over 24bp)
ATAGCCTTAATGATCCCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCT1158166.3114504848464374No Hit
ATAGCCTGCTTCTGGGTTTCTACTTCCACTATCTCGTATGCCGTCTTCTG570773.1104394843854055TruSeq Adapter, Index 6 (96% over 25bp)
ATAGCCTTAATGATCCCTGGGTTTTGCTCTGTAGATCTCGTATGCCGTCT519702.8321309810170385No Hit
ATAGCCTTAATGATCCCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCT430362.3452682104877676No Hit
ATAGCCTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAAAAAAC425682.319764317874414No Hit
ATAGCCTGCTTCTGGGTTCTACTGAACGCATCTCGTATGCCGTCTTCTGC303371.6532298936138907Illumina PCR Primer Index 3 (96% over 25bp)
ATAGCCTTAATGATCCCTGGGTTTTACTGAACGCATCTCGTATGCCGTCT235671.2842953786728604No Hit
ATAGCCTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAGAACAC222731.2137782055068789No Hit
ATAGCCTGCTTCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCCT222621.213178755039471No Hit
ATAGCCTGCTTCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCTTCTGC205491.1198279686149533RNA PCR Primer, Index 9 (95% over 24bp)
ATAGCCTGCTTCTGGGTTTCTACTCTGTAGATCTCGTATGCCGTCTTCTG168090.9160148096962748RNA PCR Primer, Index 23 (95% over 24bp)
ATAGCCTGCTTCTGGGTTTTACTGAACGCATCTCGTATGCCGTCTTCTGC128450.6999946594412904Illumina PCR Primer Index 3 (96% over 25bp)
ATAGCCTGCTTCTGGGTTGGTCTTGGCCAGACGGTGCTGAGTTGCCATTT122930.6699131450768223No Hit
ATAGCCTGCTTCTGGGTTTTGCTGAACGCATCTCGTATGCCGTCTTCTGC80990.44135903050330955Illumina PCR Primer Index 3 (96% over 25bp)
ATAGCCTTAATGATCCCTGGGTTTCTACTTCCACTATCTCGTATGCCGTC73820.40228575912772324No Hit
ATAGCCAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG60500.3296977570743329No Hit
ATAGCCAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG58440.3184716846846945No Hit
ATAGCCTGCTTCTGGGTTCTACTTCCACTATCTCGTATGCCGTCTTCTGC57450.3130766304780236RNA PCR Primer, Index 6 (96% over 26bp)
ATAGCCTGCTTCTGGGTTTTACTCTGTAGATCTCGTATGCCGTCTTCTGC56380.3072456122950561RNA PCR Primer, Index 9 (95% over 24bp)
ATAGCCTTAATGATCCCTGGGTTTTGCTGAACGCATATCGTATGCCGTCT54260.2956925669231952No Hit
ATAGCCGTCTCCCTGGGGGCAATCTGTAGATCTCGTATGCCGTCTTCTGC35390.19285956401422552RNA PCR Primer, Index 23 (96% over 25bp)
ATAGCCGGATTTCCTTTGCCATATCATGTTTTTTGCTTTGTGTTGCTTTG31090.16942650028828118No Hit
ATAGCCTTAATGATCCCTGGGTTTTGCTTCCACTATCTCGTATGCCGTCT30240.1647943830401294TruSeq Adapter, Index 6 (95% over 21bp)
ATAGCCTTAATGATCCCTGGGTTTTGCTCTGTAGATATCGTATGCCGTCT29470.16059822976827426No Hit
ATAGCCTTAATGATCCCTGGGTTTTACTCTGTAGATATCGTATGCCGTCT24970.13607525610158833No Hit
ATAGCCAGCGAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAAT24290.1323695623030669No Hit
ATAGCCTTAATGATCCCTGGGTTTCTACTGAACGCATCTCGTATGCCGTC24170.13171561633862194No Hit
ATAGCCGTCTCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCTTCC23860.13002625593047246No Hit
ATAGCCTTAATGATCCCTGGGTTTTACTTCCACTATCTCGTATGCCGTCT22120.12054403944602057TruSeq Adapter, Index 6 (95% over 21bp)
ATAGCCTTAATGATCCCTGGGTTCTACTGAACGCATCTCGTATGCCGTCT22050.12016257096676103No Hit
ATAGCCGGTGCTTTTATGTGGAGTTGCCATTTAGCATTGACAAGTAATTT21860.11912715652305651No Hit
ATAGCCTTAATGATCCCTGGGTTCTACTCTGTAGATCTCGTATGCCGTCT20810.11340512933416312No Hit
ATAGCCAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTG20030.10915448056527088No Hit
ATAGCCTGCTTCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCT18910.10305098489711796No Hit
ATAGCCAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTG18830.10261502092082132No Hit
ATAGCTTAATGATCCCTGGGTTTTGCTTAATGCATCTTGGTTCAACTCCT18750.10217905694452467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCGG255.311449E-10294.99864
GCCTGCT432650.0294.419044
ATAGCCG399850.0294.40841
ATAGCCT1386850.0294.286041
AGCCTGC433050.0294.283333
GCCTTAA941550.0294.1374
TAGCCTG434300.0294.11562
AGCCTTA945150.0293.93743
TAGCCGT389100.0293.93722
TAGCCTT948650.0293.723632
GTCTCCC386400.0292.097477
CTTCTGG437550.0291.256779
CCTGCTT437150.0290.949685
CCGTCTC389650.0290.94825
CGTCTCC390950.0290.20716
GCTTCTG439250.0290.196728
AGCCGTC391600.0290.139743
TAGCCGG10800.0289.535682
TTAATGA953700.0289.384437
CTGCTTC440900.0288.776156
Produced by FastQC (version 0.11.2)