FastQCFastQC Report
Mon 24 Jul 2017
000000000-AY8Y0_l01n03.333000000973fa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY8Y0_l01n03.333000000973fa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12195301
Sequences flagged as poor quality0
Sequence length251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAACATCCTTGGCGAATGCTTTCGCATTAGTTAGTCTTCCGTAAATC258260.211770090791527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAGG415650.0236.688169
TCCTTGG788050.0234.646489
TCTTAGG501800.0233.57449
TCCTAGG306100.0233.313549
TTTTAGG566400.0230.400479
ATCTTAG372450.0224.409948
ATTCTAG359400.0221.276438
ATTCTTG855300.0216.340068
ATCCTTG710500.0215.930368
ATCTTTG420650.0214.974958
TATTAGG147450.0208.444589
TCTTTGG615650.0205.761159
TGTTAGG113150.0205.591489
ATGTTAG97400.0199.219098
ATCCTAG266600.0198.728748
TACTAGG123450.0195.284939
TTCTTGG1143350.0193.025159
TATTGGG94350.0192.545769
AAACATC983550.0191.381124
TTATAGG230450.0191.257819
Produced by FastQC (version 0.11.2)