FastQCFastQC Report
Mon 24 Jul 2017
000000000-AY8Y0_l01n02.332000000973fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY8Y0_l01n02.332000000973fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12195301
Sequences flagged as poor quality0
Sequence length12
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTTCCCTCG6505915.33476787493806No Hit
GAGGCTCATCAT6268685.140242130965033No Hit
TAGGATTGCTCG5931534.863783189935205No Hit
ATGTGCACGACT4959554.066771291663896No Hit
TCGAGGACTGCA4586883.761186378261595No Hit
GTACATACCGGT3894653.1935661120623426No Hit
CTGCTAACGCAA3481242.8545748891314777No Hit
GTCTAATTCCGA3453902.832156418279467No Hit
TTCCTAGGTGAG3334712.7344220532154146No Hit
GGATGGTGTTGC2972922.4377586088281054No Hit
GAACCAAAGGAT2750602.255458885352645No Hit
TCCGACACAATT2700022.213983894288464No Hit
ACACCTGGTGAT2376261.9485045920555795No Hit
ACGCGCAGATAC2323441.9051928279589No Hit
CAGGCGTATTGG2300291.886210106663214No Hit
GTATGCGCTGTA2293231.8804209916590005No Hit
GCGATATATCGC2210371.8124767892157805No Hit
TGAGTCACTGGT2129571.7462217619720908No Hit
TCACGGGAGTTG2056771.6865266384158948No Hit
TAGCTCGTAACT1962561.6092755726160428No Hit
TACACGATCTAC1815711.48886034055248No Hit
TTAGGGCTCGTA1799971.4759537300473353No Hit
TCTAGCGTAGTG1778111.458028793221258No Hit
ACGTGTACCCAA1715841.406968142893726No Hit
CGATCCGTATTA1677191.3752756082035202No Hit
CGGAGCTATGGT1381401.132731369238037No Hit
AGCTGTTGTTTG1331881.0921255654124487No Hit
TCCAAAGTGTTC1287681.055882097539044No Hit
GCACACACGTTA1156960.9486932712853909No Hit
AGGCTACACGAC1008800.827203854993001No Hit
TACAGATGGCTC985520.8081145352623933No Hit
AATCCGTACAGC948810.7780127772164049No Hit
CCAGTGTATGCA942550.7728796525809408No Hit
CAGTGCATATGC896780.7353488036088653No Hit
GTTGGTCAATCT813900.667388201406427No Hit
TTACTGTGCGAT747580.6130065998371013No Hit
AATCAGTCTCGT710520.582617846004785No Hit
CCTCGTTCGACT663990.5444638061823976No Hit
GACTTGGTATTC492840.40412286666807157No Hit
GCTGATGAGCTG490580.40226969387635453No Hit
TTTTTTTTTTTT433090.3551285860021003No Hit
CTAACCTCCGCT392670.3219846726210366No Hit
ATCCCGAATTTG380530.3120300187752644No Hit
GGAGACAAGGGA217860.178642577169682No Hit
TTCTTTTTTTTT172340.1413167251878408No Hit
AACACAAGGAGT151500.12422817608191876No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGCA806.464336E-56.004741
TTCGGGC806.464336E-56.004741
TCGAGGT2000.06.004741
ACGCGCA241750.06.004741
TCGGCTC751.7324265E-46.00473981
GCACCTG1156.717164E-86.00473981
CAGGCTT852.4152994E-56.00473981
CAGGCGC751.7324265E-46.00473981
TCGAGTC550.0090859146.00473981
TCGAGGC1850.06.00473981
TCGAGCG600.0033665856.00473981
TCGCGGG600.0033665856.00473981
TGCGTCA1451.9099389E-106.00473981
TCACGGG212550.06.00332741
CGATCCG173350.06.0030081
GAGGCTC633350.06.00236941
TCGAGGA470850.06.0021891
CAGGCGT236250.06.00092741
CTGCTAA360900.06.00058031
TCCGACA273100.06.00034241
Produced by FastQC (version 0.11.2)