FastQCFastQC Report
Mon 24 Jul 2017
000000000-AY8Y0_l01n01.331000000973f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AY8Y0_l01n01.331000000973f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12195301
Sequences flagged as poor quality0
Sequence length251
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTCG745963561.1681089298247No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG8198936.7230238925632095No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG2781012.280394719244732No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCTCGTAGTCG2301541.8872350916143847No Hit
AGCTCTCAAAGTGTATATCGTCATTGCTGCGGTTAAAAAGCTCGTAGTTG1072180.8791746919571728No Hit
AGCTCTAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG859330.7046402544717839No Hit
AGCTCCAAGAGCGTATATAAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTG760450.6235598448943572No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGTTCGTAGTTG474890.38940408276925675No Hit
CTCTCCCCTCTCTTCTCTTCCCTTTTTTTCCTTTCCCCCTCTCTTCTTCT458610.3760546787652064No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTCG429480.35216842946311866No Hit
AGCTCCAAAAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTTG350170.28713518428122436No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAACGCTCGTAGTCG310730.25479485910187866No Hit
AGCTCCAATAGCGTATATTAAAGTTGCTGCGGTTAAAAAGCTCGTAGTTG298420.24470080730274718No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTAGTCG295680.24245404028978046No Hit
AGCTCCAATAGCGTATATTTAAGTTGCTGCGTTTGAAACGCTCGTAGTCG254360.20857213774387365No Hit
AGCTCCAATAGCGTATATTAACGTTGTTGCGGTTAAAAAGCTCGTAGTCG239510.1963953165239628No Hit
AGCTCCAATAGCGTATATTAAAGATGTTGCGGTTAAAAAGCTCGTAGTCG227590.18662106002959664No Hit
AGCTCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTTG227490.18653906123350297No Hit
AGCTCCAATAGCGTATATTACAGTTGTTGCGGTTAAAAAGCTCGTAGTCG226160.18544847724545707No Hit
AGCTCCACTAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTCG210770.1728288625266404No Hit
GAGTGCATGGAATAATGGAATAGGACCTCGGTTCTATTTTGTTGGTTTTC141260.11583149936192635No Hit
AGCTCCAATAGCGTATATTAAAGGTGTTGCGGTTAAAAAGCTCGTAGTCG130860.10730362456818408No Hit
AGATCCAATAGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTCG127840.10482726092615507No Hit
AGCTCCAATCGCGTATATTAAAGTTGTTGCGGTTAAAAAGCTCGTAGTCG122720.10062892256615888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCCA9771500.0244.973391
TCCAATA9639200.0244.962864
CCAATAG9642750.0244.95785
CTCCAAT9643400.0244.924773
AATAGCG9755900.0244.823917
TAGCGTA9755300.0244.808859
ATAGCGT9741900.0244.710518
GCTCCAA9758500.0244.437332
CAATAGC9798550.0240.989126
AACAAAT5611450.0240.22542245
AGCTCTC135650.0239.220141
CAAAGTG123550.0238.753277
AGCTCTA104650.0238.21041
CACAAAT274000.0237.84645245
CCACTAG38600.0234.526935
AGATCCA21300.0230.046681
AACACAT141150.0229.81198245
CCCATAG16650.0228.813555
GCTCCAC45000.0228.121962
CTCTCAA135550.0227.01563
Produced by FastQC (version 0.11.2)