FastQCFastQC Report
Tue 18 Apr 2017
000000000-AVA3A_l01n01.3310000008a5cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AVA3A_l01n01.3310000008a5cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22990894
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGTGTCGGTCTCGTAGGTATAGACACTGACGGACAAATCGATGAATT368690.16036348999738767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTG217250.045.320652
GTGTCGG11443750.045.3136566
TACATGT800850.045.261331
TGTCGGT11456150.045.260037
CCGTCCA1580850.045.197984
ACATGTG443500.045.156382
GCCGGGT818000.045.0953251
CGGGTCC606700.045.0795253
GGTCCAC3258250.045.0610925
AGGTCCA2083950.045.0426454
GTCCACG10337350.045.0403256
TCCACGA10343450.045.0124367
GTTGTGT600950.044.9723133
GGTGTCG4069100.044.9634325
CACGTGT897600.044.9608233
GGGTGTC622500.044.931214
ACCAGGT1313900.044.928961
CGTGTCG4121750.044.92495
AGGTGTC2924250.044.918094
ACTGTCC476200.044.8602143
Produced by FastQC (version 0.11.2)