FastQCFastQC Report
Thu 8 Jun 2017
000000000-AV71Y_l01n02_oat0_reprep.352000000902cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AV71Y_l01n02_oat0_reprep.352000000902cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5609359
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG815991.4546938429150282No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA402890.7182460598439144No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT268120.47798687871466244No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC239370.42673325062631934No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC198410.35371242953071824No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT181180.322995907375513No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT169420.30203094506876815No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG159220.28384704919046905No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT158710.28293785439655406No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT156240.27853449921818163No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT154760.2758960515809382No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT144540.2576765010048385No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC142490.2540218944802784No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA142340.253754484246774No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA140390.2502781512112168No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG137740.24555390375263914No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC133920.2387438564727271No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC133540.23806641721451594No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG133110.23729984121180336No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC127840.22790482834134881No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC126200.2249811431217007No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT125640.2239828115832843No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT122810.2189376718445013No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT122040.2175649659791787No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG120180.2142490790837242No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA116840.20829474455102623No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA111350.19850753000476523No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC110320.19667131306803504No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT109310.19487075082910543No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT107770.19212533909846027No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG107220.19114483490894416No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA103300.1841565141400292No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA103280.1841208594422286No Hit
GTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAGGATGTTGGCTT102460.18265901683240457No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT101310.18060887170887083No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT100790.1796818495660556No Hit
GGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGG99980.17823783430513182No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA99180.17681164639310837No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG98390.1754032858299852No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG96390.17183781604992657No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT95420.17010856320659812No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG92380.16468904914090896No Hit
GTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG92070.16413640132499988No Hit
CTGCAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCA90910.16206842885256587No Hit
ATAAGGCTGAGCTGTGATGGCGAGCGAAATATAGTAGCGAAGTTCCTGAT90590.1614979536877565No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC88780.1582712035368034No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC87790.15650629599567437No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT87760.1564528139489735No Hit
CGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCT87750.1564349866000732No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG86990.1550801080836509No Hit
GTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTTGCTTACAGCTCC86750.15465225171004388No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT84880.15131853746568905No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC84440.15053413411407612No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC83590.1490188094575512No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT82080.14632687977360692No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA81720.14568509521319636No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG80690.14384887827646617No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG79170.1411391212436216No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT79110.14103215715021983No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT79010.1408538836612169No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC78610.14014078970520516No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA78560.1400516529607037No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG78250.13949900514479463No Hit
GGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTC74120.1321363100489735No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC73080.130282265763343No Hit
GGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATT72920.12999702818093833No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGAC72910.12997920083203804No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG72670.129551344458431No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG71390.12726944379919347No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC69260.12347221848343098No Hit
GTCCTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTC69170.12331177234332835No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG68590.12227778610711135No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA68220.12161817419780051No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA68000.12122597252199406No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG66460.11848056079134887No Hit
GTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCT66190.11799922237104098No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT65640.11701871818152484No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT65230.11628779687661282No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA65070.11600255929420814No Hit
AGAAAAGCCTCTAGCGAGGTGAGAGGTGCCCGTACCGCAAACCGACACAG64970.11582428580520521No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG64770.11546773882719934No Hit
CTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAT64510.1150042277557917No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT64060.1142019970552785No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA63780.11370283128607031No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC63260.11277580914325505No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA63230.11272232709655418No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG62710.11179530495373892No Hit
GTGGAGTTGCTTAGACAACCAGGATGTTGGCTTAGAAGCAGCCACCATTT62400.11124265713782983No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC62350.11115352039332835No Hit
CAATGACTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG61290.1092638214098973No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG60980.10871117359398819No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG60920.10860420950058643No Hit
GTGCCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTG60750.10830114456928146No Hit
CTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTACCGGGTTACCGAA60350.10758805061326972No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG59540.10614403535234597No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG58870.1049496029760263No Hit
GTTAAGCAGAAGATGCGGAGCCGCAGCGAAAGCGAGTCTGAATAGGGCGC58690.10462871069582104No Hit
CGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGT58430.10416519962441341No Hit
GTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTACTATAATT58290.10391561673980931No Hit
GCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCT58070.10352341506400285No Hit
CTCCTACCTATCCTGTACAAGCTGTACCAACATTCAATATCAGGCTGCAG58060.10350558771510257No Hit
CTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATG57600.10268552966568908No Hit
GTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGAC57500.10250725617668614No Hit
CCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAG57390.10231115533878292No Hit
CAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGA57020.10165154342947205No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGAGATCG56100.1000114273306451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTCG4300.056.9767461
CGGCCTA707.2759576E-1250.01
CTGGCAT1550.049.6774181
TCCTCGA5000.047.6000022
GGCCGGT1300.045.769231
CCTCGGT1150.045.6521721
GCCGGGT3500.043.0000041
TGGTCCC2050.042.68292670
GTCGCTA1250.042.0000041
CGGTCAA4000.040.253
GGGGCCT2850.039.298251
GTCGCAT2150.039.0697671
TGCAACC1450.038.620692
GGCGCAC3800.037.7631571
GTCTCTT3650.036.4383541
GGCCTTA6250.036.41
TCCTATT1450.036.2068982
GCTAACC1450.036.2068985
ATCGGTC175800.035.8162737
TCGGTCA176500.035.6742238
Produced by FastQC (version 0.11.2)