FastQCFastQC Report
Thu 8 Jun 2017
000000000-AV71Y_l01n02_79t2_reprep.352000000902be.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AV71Y_l01n02_79t2_reprep.352000000902be.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4904029
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG719011.4661618028767773No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA346880.7073367633021745No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT240320.4900460417342557No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC211940.4321752583437007No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC164720.33588708386512395No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT157690.3215519320950182No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT149370.3045862901707963No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG142650.2908832716935401No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT138000.2814012723007959No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT133470.2721639696665742No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT133010.2712259654255715No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA125560.2560343750006372No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT124140.2531387966914551No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC123370.25156865915760285No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA120230.24516576064293258No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG118260.241148655523856No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC114120.2327066173548321No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG113650.23174822171728593No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC113330.2310956970278928No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC113200.23083060887282683No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC111280.22691546073646793No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT107620.21945220960153375No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT106430.21702563341285297No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT105550.21523119051702183No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA101360.20668719536528027No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT100640.20521901481414567No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG100300.20452570733166545No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA95150.19402413811174443No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC94080.1918422586815861No Hit
GGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGG87920.17928115841076797No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG87260.17793532623889458No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA87160.17773141227345923No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT85620.1745911372057547No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA85340.17402017810253567No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT85200.1737346985509262No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT85100.17353078458549082No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG84980.1732860878269684No Hit
GTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAGGATGTTGGCTT84280.17185869006892088No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA82350.16792315053601845No Hit
GTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG81690.16657731836414508No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG81380.16594518507129546No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG80300.16374291424459358No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT80170.1634778260895276No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT78910.1609085101250421No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC77620.15827801997092594No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC77570.15817606298820827No Hit
ATAAGGCTGAGCTGTGATGGCGAGCGAAATATAGTAGCGAAGTTCCTGAT77130.15727884154029267No Hit
CGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCT76660.1563204459027465No Hit
CTGCAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCA76510.15601457495459348No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG76460.15591261797187578No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC76290.15556596423063565No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT73070.1489999345436171No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC72480.14779684214754849No Hit
GTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTTGCTTACAGCTCC70750.14426913054551677No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG69600.14192411994301013No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT69370.1414551178225088No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA69070.14084337592620272No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT68840.1403743738057014No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT68070.13880423627184912No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG67770.13819249437554304No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG67580.13780505784121586No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA65330.13321699361892028No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC64020.13054572067171707No Hit
GGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATT63400.12928145408601785No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC62710.12787444772451387No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGAC62450.12734427141438193No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG61580.1255702199150943No Hit
GGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTC61390.12518278338076713No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG61140.12467299846717873No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA60820.12402047377778556No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG60280.12291933836443462No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA58920.12014610843451375No Hit
GTCCTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTC58840.11998297726216545No Hit
CTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAT57900.11806618598707308No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT57830.11792344621126834No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG57170.11657761403939496No Hit
GTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCT56510.11523178186752159No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC55930.1140490808679965No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA55870.11392673248873529No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA55750.11368203573021286No Hit
AGAAAAGCCTCTAGCGAGGTGAGAGGTGCCCGTACCGCAAACCGACACAG55330.11282559707538435No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG55310.11278481428229727No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA55040.1122342465756218No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT54650.1114389821104239No Hit
GTGCCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTG54360.11084763161066134No Hit
GTGGAGTTGCTTAGACAACCAGGATGTTGGCTTAGAAGCAGCCACCATTT53900.1099096273696587No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT53620.1093386682664397No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC53290.10866575218050302No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG53180.1084414468185241No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG53020.10811518447382755No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGAGATCG52510.10707522325010721No Hit
GCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCT52310.10666739531923648No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG51980.1059944792332998No Hit
CTCCTACCTATCCTGTACAAGCTGTACCAACATTCAATATCAGGCTGCAG51870.10577017387132091No Hit
CTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTACCGGGTTACCGAA51860.10574978247477737No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG51780.1055866513024291No Hit
CAATGACTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG51600.10521960616464544No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC51300.10460786426833937No Hit
CGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGT50370.10271146438979052No Hit
CTGGACATGGGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATG50180.10232402785546332No Hit
CCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAG50070.10209972249348445No Hit
CCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGC49370.10067232473543691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGGT1550.047.4193531
TGGTCCC1350.046.66666870
GTCCTCG3500.045.01
GCCGGGT2600.043.0769231
TCCTCGA3800.041.4473652
GGGGACC602.602137E-740.8333361
TACCATA801.4279067E-939.37499616
CTGGCAT1250.039.21
ACTCTAG1800.038.8888855
TACTCTA1800.038.8888854
GTTACTA1450.038.620693
GGGTACA1400.037.54
TAGATCT951.891749E-1036.84210244
GGCCTTA4400.036.5909081
TCGGTCA148850.035.7641938
ATCGGTC148700.035.72965637
CGGTCAC151300.035.30072439
TGTACTC2100.035.02
TTACTAC1600.034.9999964
GGTCACT152350.034.85067440
Produced by FastQC (version 0.11.2)