FastQCFastQC Report
Thu 8 Jun 2017
000000000-AV71Y_l01n01_oat2_reprep.351000000902db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AV71Y_l01n01_oat2_reprep.351000000902db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6355061
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG811491.2769192931428983No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA437700.6887424054623551No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT328340.5166590847829785No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC287870.45297755599828227No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC226730.3567707689981261No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT198060.31165711863347967No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT188690.29691296432874525No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG187510.29505617648674026No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT182310.2868737215897692No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT181190.28511134668888305No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA169570.26682670709219003No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT166190.26150811140915875No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC165720.2607685433703941No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT163050.25656716749060315No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG159570.2510912169057071No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC159470.25093386200384227No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC157200.24736190573151068No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC154820.24361685906712777No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA153490.24152403887232554No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG150060.23612676573836192No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC145770.22937624044836075No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT137880.21696093869122576No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA135450.21313721457591042No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT135380.2130270661446051No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG134420.21151645908670272No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT132140.20792876732418458No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA131660.2071734637952334No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC125400.19732304693849517No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT122690.1930587290979583No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA122590.1929013741960935No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT121140.19061972811905345No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT119510.18805484321865676No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG117650.18512804204397093No Hit
GTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAGGATGTTGGCTT115080.18108402106604485No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG112900.17765368420539157No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT111150.17489997342275707No Hit
ATAAGGCTGAGCTGTGATGGCGAGCGAAATATAGTAGCGAAGTTCCTGAT109220.17186302381676588No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA108720.17107624930744175No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG108130.17014785538643926No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT107870.1697387326415907No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA106590.16772458989772088No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC106170.1670636993098886No Hit
CTGCAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCA104050.16372777539035424No Hit
GTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG103750.16325571068475975No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT103680.16314556225345436No Hit
CGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCT102030.1605492063726847No Hit
GGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGG99500.15656812735550454No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG99370.15636356598308024No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC98450.15491590088592383No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT98240.15458545559200768No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG98000.1542078038275321No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC95940.15096629284911664No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT95810.15076173147669236No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA95730.15063584755520049No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT95110.14966024716363857No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG94000.14791360775293896No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT91630.14418429657874252No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG87600.1378428940335899No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG86820.13661552579904426No Hit
GTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTTGCTTACAGCTCC84030.13222532403701553No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC83420.13126545913564008No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA82970.13055736207724836No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC82370.12961323266605937No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA81150.12769350286330847No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA80910.1273158510988329No Hit
GGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTC80830.12718996717734102No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG80260.1262930442367115No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC80050.12596259894279535No Hit
GGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATT79780.12553774070776033No Hit
AGAAAAGCCTCTAGCGAGGTGAGAGGTGCCCGTACCGCAAACCGACACAG79740.1254747987470144No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG79260.12471949521806322No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT79210.12464081776713079No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGAC78650.12375963031668775No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA78490.12350786247370402No Hit
CTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAT77970.12268961698400692No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA77920.1226109395330745No Hit
GTCCTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTC77880.12254799757232858No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT77710.12228049423915836No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG76910.12102165502423973No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG75830.1193222220840996No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG75540.11886589286869158No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA75120.1182050022808593No Hit
GTGCCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTG74780.11766999561451888No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG72810.11457010404778176No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG72630.11428686522442506No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC71580.11263463875484438No Hit
GTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCT71570.11261890326465789No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC71350.11227272248055527No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC71330.1122412515001823No Hit
GTGGAGTTGCTTAGACAACCAGGATGTTGGCTTAGAAGCAGCCACCATTT71000.11172198032402836No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT70600.11109256071656903No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG70070.11025857973668546No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG69610.10953474718810724No Hit
CAATGACTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG68190.10730030758162667No Hit
CTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTACCGGGTTACCGAA66880.10523895836719742No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG66850.10519175189663797No Hit
CTCCTACCTATCCTGTACAAGCTGTACCAACATTCAATATCAGGCTGCAG66630.10484557111253535No Hit
CCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAG66250.104247622485449No Hit
CAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGA65240.10265833797661424No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT65140.10250098307474939No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG65090.10242230562381699No Hit
GAGAATCCTAAGGTGATCGAGAGAACTCTCGTTAAGGAACTCGGCAAAAT64920.10215480229064677No Hit
GTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTACTATAATT64700.10180862150654414No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT64620.1016827375850523No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA64410.10135229229113615No Hit
GTGATGGCGAGCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAA63820.10042389837013367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTCG4650.059.4633671
GTCGCAT2400.053.9592481
GGCCTCT850.053.5303151
TCCTCGA5700.049.1236342
CTGGCTA1450.043.449011
GCCGGGT3650.042.1924931
TCCTATT1500.042.000712
GGCCGGT1600.041.56321
GGCCTTA6600.040.8340231
GGGGCCT1800.040.8340231
CTGGCAT1950.039.4878431
AGCCGGT2750.039.455211
TCCTGGC3500.038.9997377
GGGCATT4150.038.7958371
GCTAACC1650.038.181565
GTGGCAT1600.037.188131
TGGCTAC1600.037.188132
GGGGACC951.891749E-1036.8427281
GTGGGTC400.002420054535.000591
CCTCGGT1102.3646862E-1135.000591
Produced by FastQC (version 0.11.2)