FastQCFastQC Report
Thu 8 Jun 2017
000000000-AV71Y_l01n01_79t2_reprep.351000000902b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AV71Y_l01n01_79t2_reprep.351000000902b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4904029
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG595451.2142057071848475No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA332150.6773002361935462No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT242940.49538858762866206No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC212680.4336842216879223No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC169700.34604199934380486No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG149170.3041784622399256No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT145550.2967967766911656No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT144450.2945537230713766No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT139130.2837055001102155No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC138160.28172753464549255No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG134120.273489410441904No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC133880.27300001692485915No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT133590.2724086664250966No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC124490.25385249557047884No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT123370.25156865915760285No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT122900.25061026352005666No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA122720.25024321838227304No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA122510.2498149990548588No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC115960.23645863431884273No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG113020.23046356373504318No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG111560.22748641983968693No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC110280.2248763210821143No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA108390.22102234713538604No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT103070.2101741241742249No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT101540.20705424050306392No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT100710.20536175458995043No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA100570.20507627503834092No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT98640.20114073550543848No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC96590.19696049921401362No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA94930.19357552738778666No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT94800.19331043923272068No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC94440.1925763489571534No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG90230.18399157101232477No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG89700.18291082699551736No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT89250.18199321415105824No Hit
GTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAGGATGTTGGCTT88300.18005603147942234No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG87830.17909763584187613No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT84660.17263356313757525No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT84580.17247043196522696No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA83890.17106342560372298No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG83250.16975837622493667No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT82200.1676172795878654No Hit
ATAAGGCTGAGCTGTGATGGCGAGCGAAATATAGTAGCGAAGTTCCTGAT80070.16327391212409226No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA79490.16209121112456717No Hit
CTGCAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCA77760.15856349952253546No Hit
GTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG77470.1579721490227729No Hit
CGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCT74940.15281312569725833No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC74590.15209942681823457No Hit
GGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGG74290.1514876849219285No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT73480.14983598180190208No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC73430.14973402481918438No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG73400.14967285062955377No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA72680.1482046700784192No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT71150.1450847864072582No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG70800.14437108752823444No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT70150.1430456467529046No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG69590.1419037285464666No Hit
GTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTTGCTTACAGCTCC69570.14186294575337952No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG68820.14033359101261433No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT68590.13986458889211298No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG67110.13684666220366967No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG63510.12950575944799675No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG63190.12885323475860358No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC63130.12873088637934238No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC63070.12860853800008115No Hit
GGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTC62940.1283434498450152No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA62880.128221101465754No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA62680.12781327353488325No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG61800.1260188306390521No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA61450.12530513176002833No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG60770.12391851679506788No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC60160.1226746416059122No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA59310.12094137289971164No Hit
GGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATT58670.11963632352092535No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA58580.11945280095203353No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGAC58470.11922849559005463No Hit
CTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAT58440.11916732140042402No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT58230.11873910207300976No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT58190.11865753648683562No Hit
GTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCT58180.11863714509029208No Hit
AGAAAAGCCTCTAGCGAGGTGAGAGGTGCCCGTACCGCAAACCGACACAG57500.11725053012533165No Hit
GTCCTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTC57490.1172301387287881No Hit
CAATGACTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG56410.11502786790208622No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG56160.11451808298849782No Hit
CAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGA55940.11406947226454003No Hit
GTGGAGTTGCTTAGACAACCAGGATGTTGGCTTAGAAGCAGCCACCATTT55460.11309068523045031No Hit
GTGCCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTG55070.11229542076525241No Hit
GTTAAGCAGAAGATGCGGAGCCGCAGCGAAAGCGAGTCTGAATAGGGCGC54920.11198954981709937No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT54700.11154093909314158No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA54600.11133702512770621No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC54490.11111271976572733No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG54260.110643717645226No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG53080.10823753285308876No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG52730.107523833974065No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC52660.10738109419826025No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT52660.10738109419826025No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA52490.10703444045702014No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC52350.10674896090541064No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT51650.10532156314736311No Hit
CCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAG50920.10383299119968499No Hit
GTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTACTATAATT50590.1031600751137483No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG50300.10256872461398575No Hit
CTCCTACCTATCCTGTACAAGCTGTACCAACATTCAATATCAGGCTGCAG50150.10226285366583272No Hit
CTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTACCGGGTTACCGAA49720.10138602361446068No Hit
GTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTAC49390.100713107528524No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG49300.10052958495963217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTCG2450.049.999891
GCTAACC1150.048.6955455
CTGGCAT1200.046.6665651
GGGGCCT1700.045.2940181
CCTCGGT1050.043.3332371
GGCCGGT1700.041.176381
GTCGCAT1550.040.6450731
GTCCTTT3900.039.487091
TCCTCGA3050.039.0163082
GTGGCAT1200.037.9165841
CTACGTT654.874528E-737.6922261
GCCGGGT2150.037.4417761
CTCTTAT1500.037.333258
GGCCTTA4700.037.233961
TCCTGGC3150.036.6665847
GCGCACG4000.035.8749242
GGCGCAC4100.035.853581
CGGGTTC4500.035.77772
TAGTCGT708.7077933E-734.9999220
GGGTTCC4450.034.6066673
Produced by FastQC (version 0.11.2)