FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n02_79t2b_prelim.35200000086e3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n02_79t2b_prelim.35200000086e3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190351
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA448122.045882144003404No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG280071.2786535126105358No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC242531.1072654565409836No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG240901.0998237268821298No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT229691.0486447149338167No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG173440.7918365595285869No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT158090.7217564673424487No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT115190.5258974474867271No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA113740.51927750392517No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA105190.4802426643035751No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG96800.44193830121291067No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT84930.3877460735745093No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA81770.37331916208863325No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT76020.34706766175832093No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT73900.3373888477234927No Hit
GGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAG71650.32711652150728354No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG68490.31268961002140755No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT68310.3118678239241108No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG67750.3093111560658543No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT66830.30511091601300433No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG65440.29876490115054616No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA64520.29456466109769625No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT63880.29164275497397446No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA61950.2828313818196262No Hit
CGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAA58610.2675826842364534No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT57230.26128232415717845No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA56040.2558494049583834No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT55800.25475369016198773No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCA54260.24772285355178233No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT51760.23630915775599437No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG51090.23325028728272318No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA49540.22617379588933464No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC48180.21996474537642596No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG47920.218777721013664No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA47790.21818420883228307No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT47640.21749938708453576No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT47510.2169058749031548No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA47060.21485140965991295No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA46600.212751289633488No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC46560.21256867050075534No Hit
GACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATC46290.21133599135481027No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA46100.21046855047433038No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT44540.2033464042977587No Hit
CTGTGTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCG44240.2019767608022641No Hit
ATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATG43850.2001962242581212No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG42630.19462634070977664No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG42350.1933480067806484No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT41690.19033479109056037No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC41570.18978693369236255No Hit
GACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAG41140.18782377801548703No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC40510.18494752667494843No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC40330.1841257405776517No Hit
CGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAG40170.18339526404672127No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT39660.18106687010438052No Hit
CTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAG38390.17526871264012023No Hit
CTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAAGTG37680.17202722303411644No Hit
CTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTG37600.17166198476865124No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTGATCCCGTTTCGCT37140.16956186474222623No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG36340.16590948208757408No Hit
CGGGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTAC36280.16563555338847516No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC36100.16481376729117844No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT36050.16458549337526268No Hit
TGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATAT35240.16088745593742737No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT34700.15842209764553716No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT34330.15673287066776057No Hit
GAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCTC34280.15650459675184478No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC33980.15513495325635024No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT33980.15513495325635024No Hit
CTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTAGTCGGGACCTAA33350.15225870191581167No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT33150.15134560625214863No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG32830.14988465319028776No Hit
CTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGT32640.14901721230980788No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT32470.1482410809956943No Hit
GCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGT32380.14783018794704594No Hit
TTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTC32360.14773887838067962No Hit
CGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGA32080.14646054445155138No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT32050.14632358010200192No Hit
CTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCGCCTACCTG31800.1451822105224231No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA31310.14294512614644866No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG31060.1418037565668699No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT30810.14066238698729108No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG30570.13956667219089544No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG30540.13942970784134598No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA30340.13851661217768294No Hit
CTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATA30150.13764917129720305No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT30000.13696434954945577No Hit
CTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT29820.13614256345215903No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT29680.1355033964875949No Hit
CTCATACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTG29680.1355033964875949No Hit
GTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCG28830.131622739917027No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG28810.1315314303506607No Hit
CCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTG28640.13075529903654712No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA28500.130116132071983No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA28250.1289747624924042No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT27890.1273311902978107No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA27640.12618982071823193No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA27580.125915892019133No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC27500.12555065375366778No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT27380.12500279635546996No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA27220.12427231982453954No Hit
CTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAGAGT27180.12408970069180693No Hit
CTTGGTTTGGGCTGATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT26970.12313095024496074No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA26840.12253743806357976No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC26840.12253743806357976No Hit
GGGAAACCCACCACTCGTAATGGAGTGGTATCCATATCTGAATTCATAGG26610.12148737805036727No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG26190.11956987715667489No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC25660.11715017364796784No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC25430.11610011363475535No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT25190.11500439883835968No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA25140.11477612492244395No Hit
CTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCTTCT24720.11285862402875156No Hit
CAAGAGTCCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCG24420.111488980533257No Hit
GGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTAC24380.11130636140052438No Hit
TTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCT24280.11084981356869288No Hit
AGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGC24180.11039326573686135No Hit
CCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTG24130.11016499182094558No Hit
ATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAA24110.1100736822545793No Hit
ATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGGGTTTCCCCAT24060.10984540833866353No Hit
CTACGACCACGTACTCATGCGCCCTATTCAGACTCGCTTTCGCTGCGGCT23980.10948017007319831No Hit
GGAAGAGAAAGCAAATGCGATTCCCTGAGTAGCGGCGAGCGAAACGGGAT23870.10897796745818365No Hit
CAGGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTA23760.10847576484316897No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG23630.10788225266178801No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA23620.10783659787860485No Hit
ATTGAATGTTGGTACAGCTTGTACAGGATAGGTAGGAGCCTTGGAAACCG23570.10760832396268909No Hit
GTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCT23560.10756266917950592No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC23180.10582778741854618No Hit
CGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGGGCTG22460.10254064302935922No Hit
ATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCT22360.10208409519752772No Hit
CTTTACTGCAGCCTGATATTGAATGTTGGTACAGCTTGTACAGGATAGGT22270.10167320214887934No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG22160.10117099953386466No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG22100.10089707083476575No Hit
GTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCGGGCT22010.10048617778611738No Hit
CTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTC22000.10044052300293423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTC352.9770308E-760.0005872
GTTTGAT4300.053.721461
TGGTCCC750.051.3338370
TGATCCT4450.050.3341264
TCCTGGC4450.050.3341267
TCGGTCG352.0877002E-550.00163338
CTACGTT651.8553692E-1048.4620131
ACCTTGT551.313947E-744.542846
CGAGTAT404.592196E-543.75642449
GGCCTTA1050.043.333761
GTCCTTT2300.042.609111
GGCGTCC503.4821041E-642.000412
CTCTAGT503.4821041E-642.000411
TCCGGAT41050.041.608270
CTCCGGA41550.041.1917469
TCTCCGG41750.041.07824768
TTTGGAG602.6015368E-740.83279862
TTGGTTA701.9310392E-839.996748
CGGTGCA1250.039.2003862
GGCGCAC3850.039.0912931
Produced by FastQC (version 0.11.2)