FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n02_79t2a_prelim.35200000086df5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n02_79t2a_prelim.35200000086df5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735705
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG443821.6223240444419262No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA301641.1026042647142142No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT231850.8474963492043184No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG186820.6828952683129211No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT163050.5960072449332073No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG151440.5535684585874574No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT121300.44339576087333976No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC106320.3886383948561706No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT95320.34842938109189403No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT93240.34082622212555813No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT91990.33625701601598124No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC89690.3278496767743598No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA80820.2954265902208023No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA78840.28818896774323255No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT78020.28519156853535016No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA77670.28391219082466856No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG76910.28113411351004586No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC76900.28109755986116924No Hit
CTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTAGTCGGGACCTAA75770.27696699753811177No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG75040.27429858117011885No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT74380.2718860403442623No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA73930.27024112614481455No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC72000.2631862719116279No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC70380.25726458079361625No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG70020.2559486494340581No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT69580.2543402888834871No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT67590.24706611275704068No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC67170.24553085950422285No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC64840.23701385931597158No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT64560.23599035714742633No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT61960.22648640843950643No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG60200.22005296623722223No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG59750.21840805203777452No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG58520.2139119532259509No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA58510.2138753995770743No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA57690.21087800036919185No Hit
CTAAGGTCCCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAG57290.20941585441412724No Hit
ATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAA56780.20755161832141988No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC56460.2063819015573682No Hit
CTCAAGGTAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCT55590.2032017341051027No Hit
CTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGAAATTC53840.1968048455516951No Hit
CTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTACCGGGTTACCGAA53420.19526959229887725No Hit
CTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCCTCAAGGTAAGAGTCTTG51750.18916513293648257No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC51360.18773954063029458No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT50730.18543666075106782No Hit
CTCCTACCTATCCTGTACAAGCTGTACCAACATTCAATATCAGGCTGCAG50040.18291445897858138No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA50020.18284135168082816No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTGATCCCGTTTCGCT49870.18229304694767895No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG49400.18057502545047804No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT48720.17808937732686822No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA48530.17739485799821253No Hit
ATCCCCTCCACCGAATGCCTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCA48420.17699276786056975No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT47720.17443401243920673No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT47510.1736663858127978No Hit
ATCCTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACC47490.17359327851504458No Hit
CTCCGAAATAGCTTTAGGGCTAGCCTCAAGGTAAGAGTCTTGGAGGTAGA47100.17216768620885659No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT45990.1681102311835523No Hit
ATCCTTTTCCACTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGC45930.16789090929029263No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG45840.1675619264504031No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA45080.16478384913578037No Hit
GTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAGGATGTTGGCTT44400.16229820101217055No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT43920.16054362586609303No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT43770.1599953211329438No Hit
CTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCG42050.15370809352616602No Hit
GTCAGAGCCCGTTAACGGGTGATGGCGTGCCTTTTGTAGAATGAACCGGC41900.1531597887930168No Hit
CTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTG41890.15312323514414017No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA41690.15239216216660786No Hit
GGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCT41650.1522459475711014No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG41630.1521728402733482No Hit
ATAAGATCCGTAGTCGAAAGGGAAACAGCCCAGACCGCCAGCTAAGGTCC41320.15103967715817312No Hit
CTAAAAGGTAACGGAGGCGCCCAAAGGTTCCCTCAGAATGGTTGGAAATC41230.1507106943182836No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA41070.15012583593625775No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA40790.14910233376771254No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG40740.14891956552332944No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG40740.14891956552332944No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT40620.14848092173681007No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG40600.14840781443905685No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG39470.14427725211599934No Hit
CCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAG39290.1436192864362203No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA39270.14354617913846707No Hit
CTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGT39230.14339996454296058No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG38710.1414991748013766No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG38670.14135296020587015No Hit
CTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCTTCT38590.14106053101485722No Hit
CAATGACTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG38280.13992736789968216No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT37970.1387942047845071No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA37410.13674720044741667No Hit
CTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTC37300.1363451103097739No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC36990.13521194719459884No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG36890.13484641070583267No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA36660.13400567678167055No Hit
CTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCGAAAGGG36630.13389601583504068No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC36560.13364014029290439No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT36220.13239731623109946No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG36200.13232420893334626No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC35810.13089861662715827No Hit
ATCGCATTTGCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCG35500.1297654535119832No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC35490.1297288998631066No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT35340.12918059512995736No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA35060.12815709296141214No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT34770.12709703714399032No Hit
CTCATACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTG34510.12614664227319833No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG34320.12545212294454264No Hit
ATTGGACTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAAT34010.12431895982936757No Hit
ATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGC33930.12402653063835466No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG33910.12395342334060142No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC33670.12307613576756267No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT33260.12157743616362145No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC33190.12132156062148515No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA33130.12110223872822544No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT32970.1205173803461996No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC32850.12007873655968022No Hit
CTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAAGTG32780.11982286101754393No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG32740.11967664642203747No Hit
CTTTTCCACTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGT32600.11916489533776485No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG32380.11836071506247932No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCA32260.11792207127595995No Hit
CTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTG32170.11759308843607041No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT32050.11715444464955103No Hit
ATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCAC31940.11675235451190827No Hit
GTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGAC31890.1165695862675252No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG31840.11638681802314212No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA31810.11627715707651226No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC30990.11327975786862984No Hit
GTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTACTATAATT30890.11291422137986369No Hit
CTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAT30420.11119619988266279No Hit
ATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCC30340.11090377069164986No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT30290.1107210024472668No Hit
CTTTACTGCAGCCTGATATTGAATGTTGGTACAGCTTGTACAGGATAGGT29990.10962439298096835No Hit
CTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAG29790.10889332000343604No Hit
CTTAGACAACCAGGATGTTGGCTTAGAAGCAGCCACCATTTAAAGAGTGC29540.10797947878152067No Hit
CTGAATTCATAGGATATGAGAAGGCAGACCCGGGGAACTGAAACATCTAA29470.10772360323938436No Hit
ATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCTTCTCC29380.10739462039949482No Hit
CTGTGTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCG29330.10721185215511175No Hit
CTGCAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCA29080.1062980109331964No Hit
GCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAA29050.10618834998656654No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC29040.10615179633768992No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT28920.10571315255117053No Hit
CGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCT28900.1056400452534173No Hit
ATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATG28400.10381236280958657No Hit
CTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGC28210.10311784348093088No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG28190.10304473618317764No Hit
GTGGATCCTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAG28020.10242332415227519No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA27840.10176535847249613No Hit
GTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGCGGAGTG27560.10074185630395091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGGC2800.057.4960987
GTGACTA252.3889708E-456.01257746
GTTTGGT252.3919933E-455.99824556
AACATTA200.00694889552.5079534
GTCCTTT3250.051.6887971
TGATCCT3350.050.145854
TAGTGGT451.6840477E-646.67714749
TCGATCG8950.046.142123
GGCTCAG3550.045.34903311
TGGTCCC851.8189894E-1245.2951870
ATTGTCC404.595841E-543.75102669
CTGGCAT1050.043.330391
TACCGCT11700.042.1804869
CACTAGC503.4834084E-641.99868443
GTTTGAT3950.041.6427421
CGATCGA9750.041.6382034
TCCGGAT26050.041.51729270
CGCACGG3550.041.405641
CTCCGGA26400.041.36460569
TCTCCGG26600.041.05359668
Produced by FastQC (version 0.11.2)