FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n02_79t0b_prelim.35200000086e2d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n02_79t0b_prelim.35200000086e2d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819156
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA210061.1547113056824154No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC177230.9742430006002784No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG147380.8101559184588898No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG137960.758373663391155No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT127180.6991154139611996No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT105500.5799392685399163No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG76890.42266853419937594No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA73060.40161481478223965No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT71580.39347917385864656No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT64690.3556044671265136No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT62090.3413121249634446No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA58450.3213028459351479No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA57390.3154759679763583No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA52440.2882655473197461No Hit
GGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAG50860.2795802009283426No Hit
GACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAG50440.2772714379635391No Hit
GACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATC50350.27677670304250984No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG50100.2754024393729839No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT50080.27529249827942187No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT49380.2714445600047495No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC47970.26369371290862353No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA47580.2615498615841632No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG45920.25242475081851146No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT43250.23774761482797516No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCA43030.2365382627987924No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT40690.2236751548520303No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG40340.22175118571469407No Hit
CTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATA40290.22147633298078892No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT40290.22147633298078892No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG39150.21520969064775095No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG36910.20289628816879918No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA36710.20179687723317846No Hit
GGGAAACCCACCACTCGTAATGGAGTGGTATCCATATCTGAATTCATAGG36580.20108226012502503No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC36500.20064249575077672No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA36470.20047758411043362No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA35630.1958600581808267No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG35120.19305656029499396No Hit
CGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAA34650.19047294459628533No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT34320.18865891655251116No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT34060.18722968233620427No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT33400.18360162624865595No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT33330.18321683242118872No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT33100.18195250984522493No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT32650.17947883524007838No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA31620.17381686892163178No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC31370.17244260525210592No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG31070.17079348884867487No Hit
TTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTC30560.16798999096284212No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT30270.1663958451061921No Hit
GGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTAGG29230.16067890824096448No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT28030.15408244262724033No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG27840.15303800223840067No Hit
GAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCAC27370.15045438653969204No Hit
GCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGT27360.15039941599291098No Hit
CAGGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTA27330.15023450435256788No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA27110.14902515232338515No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA26680.14666141881180064No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC26410.1451772140487127No Hit
GGAAGAGAAAGCAAATGCGATTCCCTGAGTAGCGGCGAGCGAAACGGGAT26400.14512224350193167No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG26260.14435265584699716No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG26100.14347312709850063No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA26070.14330821545815753No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA25330.13924039499636096No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA25020.13753630804614886No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT25000.1374263669525868No Hit
GGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTAC24800.13632695601696612No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT24730.13594216218949887No Hit
CGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAG24690.13572228000237474No Hit
GTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGACC24370.13396322250538162No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG24140.1326988999294178No Hit
ATCGCATTTGCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCG23820.1309398424324247No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT23710.13033516641783333No Hit
GAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCTC23530.1293456965757747No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC23400.12863107946762126No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG23200.12753166853200054No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC23000.12643225759637985No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG22720.12489308228651089No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT22590.12417846517835743No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC22270.12241940768136432No Hit
GGACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGT22000.12093520291827639No Hit
TTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCT21920.12049543854402811No Hit
CTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTC21860.12016561526334191No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG21810.11989076252943671No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT21640.11895626323415913No Hit
CGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGT21580.11862643995347294No Hit
CGGGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTAC21430.11780188175175742No Hit
CTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT21430.11780188175175742No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT21200.1165375591757936No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT20950.11516329550626775No Hit
ATAAGATCCGTAGTCGAAAGGGAAACAGCCCAGACCGCCAGCTAAGGTCC20850.11461359003845739No Hit
GTGGGATACTACCCTGGCTGTATTGACCTTCTAACCCGCCGCCCTTATCG20840.11455861949167635No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA20540.11290950308824532No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT20490.11263465035434014No Hit
GGGAAATCTCATCTTGAGGGGGGCTTCATGCTTAGATGCTTTCAGCACTT20360.1119200332461867No Hit
GTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAA20290.11153523941871944No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG20240.11126038668481428No Hit
CTTGGTTTGGGCTGATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT20070.11032588738953669No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC19790.10878671207966771No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA19750.10856682989254357No Hit
GTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCG19650.10801712442473323No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT19640.10796215387795219No Hit
CCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTG19570.10757736005048496No Hit
CTTAGATGCTTTCAGCACTTATCCCGTCCGCACATAGCTACCCAGCGATG19520.10730250731657977No Hit
CTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAGAGT19500.1071925662230177No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG19260.10587327310027288No Hit
ATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAG19190.10548847927280562No Hit
ATTGAATGTTGGTACAGCTTGTACAGGATAGGTAGGAGCCTTGGAAACCG19020.10455397997752804No Hit
AGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGC18920.1040042745097177No Hit
CGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCT18900.10389433341615562No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC18890.10383936286937458No Hit
GGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAAC18820.10345456904190735No Hit
GTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG18820.10345456904190735No Hit
GGAAATTTGAGAGGAGCTGTCCTTAGTACGAGAGGACCGGGATGGACGCA18700.10279492248053493No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT18620.10235515810628666No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA18500.10169551154491424No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC18480.10158557045135216No Hit
CGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACC18290.10054113006251252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAG150.002224974670.001164
GGCGCAC2100.046.6661151
GTGCATT1200.040.832851
CGGGAGT602.601173E-740.832851
TAGTCGT602.601173E-740.8328520
ACGCCTA350.001255864540.0006363
CATTACT350.00125620439.9984343
CTCCGGA25400.039.9601669
TCCGGAT25850.039.26452670
TCTCCGG26150.038.94791468
TGGTCCC459.2046546E-538.8884370
TGCAACC459.2046546E-538.888432
TCCTATT556.7018973E-638.181372
CTCGGGT556.7018973E-638.181371
CGGCGTC1051.4551915E-1136.6662331
TCCTGGC2200.036.5894747
TCGATCG6700.036.5667343
GCCGGGT1102.3646862E-1134.999591
ATTAGTC708.704428E-734.99958818
GGCGTCC1003.274181E-1034.9995842
Produced by FastQC (version 0.11.2)