FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n02_0at2b_prelim.35200000086e10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n02_0at2b_prelim.35200000086e10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764110
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA339151.922499163884338No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG223861.2689684883595695No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG216391.2266241901015242No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG200261.1351899824840854No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT139980.7934879344258579No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC112220.6361281325994411No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA99180.5622098395224787No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG81260.4606288723492299No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT80840.4582480684311069No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT78110.44277284296330727No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT76870.435743802824087No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT66510.3770173061770524No Hit
CTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAAGTG65940.37378621514531407No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT65490.371235353804468No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG63630.36069179359563747No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT60710.34413953778392503No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC59400.3367136969916842No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA57050.32339253221171016No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT55160.31267891458015656No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT52400.29703363168963387No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG51840.29385922646546986No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC49220.27900754488098817No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT49200.27889417326583943No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT48060.27243199120236267No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC47580.2697110724387935No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT46110.2613782587253629No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC45630.25865733996179374No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT45450.2576369954254553No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT45160.25599310700579897No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC44610.25287538758920924No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG44350.25140155659227603No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG44340.25134487078470164No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG42790.2425585706106762No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA41340.23433912851239433No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA41280.2339990136669482No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG41130.23314872655333285No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT40930.2320150104018457No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA39230.2223784231142049No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA39120.22175487923088696No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA37420.21211829194324616No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG36820.20871714348878473No Hit
GTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAA35630.20197153238743615No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG34750.19698318132089268No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG34620.19624626582242605No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA34500.19556603613153375No Hit
GCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGT34500.19556603613153375No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCA33760.1913712863710313No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA33550.19018088441196979No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT33390.18927391149078004No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC32910.18655299272721088No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT32780.18581607722874424No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC32740.1855893339984468No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT32470.18405881719393916No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG31560.1789004087046726No Hit
CGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAA31540.1787870370895239No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT30980.17561263186535989No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT30090.17056759499124205No Hit
GACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATC29520.16733650395950367No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA29460.1669963891140575No Hit
CTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTAGTCGGGACCTAA28660.1624615245081089No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG28480.16144117997177046No Hit
ATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATG28380.16087432189602688No Hit
GGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTAC28240.1600807205899859No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT27860.1579266599021603No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA27510.15594265663705778No Hit
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA27310.15480894048557065No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG27280.15463888306284757No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA26920.1525981939901707No Hit
GTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCG26730.1515211636462579No Hit
ATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGGGTTTCCCCAT26450.1499339610341759No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG26170.14834675842209386No Hit
GTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACT26040.14760984292362722No Hit
CGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGA25980.14726972807818106No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT25810.14630606934941698No Hit
CGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAG25670.14551246804337598No Hit
CTCCTCTTCTGCACTCAAGTTCCCCAGTTTCCAATGACCCTCCCCGGTTG25550.14483223835248368No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT25410.14403863704644268No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA25350.14369852220099652No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT25320.14352846477827347No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA25140.14250812024193504No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG24940.14137440409044788No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG24500.13888022855717613No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC24380.13819999886628384No Hit
CCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTG23800.1349122220269711No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC23790.13485553621939675No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG23740.13457210718152496No Hit
ATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAA23700.13434536395122754No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT23420.13275816133914553No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT23320.13219130326340195No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC23210.131567759380084No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT22970.13020729999829941No Hit
ATCCCCTCCACCGAATGCCTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCA22950.13009392838315073No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA22880.1296971277301302No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC22520.12765643865745332No Hit
GTGTGTACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGAT22330.12657940831354053No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA22310.12646603669839182No Hit
CTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTCGGTGG22100.12527563473933032No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA22020.12482214827873545No Hit
CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGA21890.1240852327802688No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC21810.12363174631967394No Hit
CAGGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTA21770.12340500308937652No Hit
CTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATA21580.12232797274546373No Hit
GATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCAC21470.12170442886214579No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA21380.12119425659397656No Hit
GGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAG21380.12119425659397656No Hit
GGGGCTTCATGCTTAGATGCTTTCAGCACTTATCCCGTCCGCACATAGCT21190.12011722625006377No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA21140.11983379721219199No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC21020.11915356752129969No Hit
ATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCT20830.11807653717738689No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT20790.11784979394708947No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA20780.11779310813951512No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG20710.11739630748649461No Hit
GACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAG20380.11552567583654082No Hit
CTTGGTTTGGGCTGATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT20350.11535561841381774No Hit
GTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTAC20280.11495881776079724No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA20240.11473207453049979No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA20070.11376841580173572No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG19990.11331492934114086No Hit
CTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCGCCTACCTG19550.11082075380786911No Hit
GGGAAACCCACCACTCGTAATGGAGTGGTATCCATATCTGAATTCATAGG19390.10991378088667941No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC19370.10980040927153069No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT19310.10946029442608454No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG19250.10912017958063838No Hit
CCCTAGTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGC19240.10906349377306404No Hit
ATCGCATTTGCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCG19060.10804314923672562No Hit
GAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCTC18940.10736291954583331No Hit
GTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTT18900.10713617631553589No Hit
TGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATAT18870.10696611889281281No Hit
CTCATACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTG18780.10645594662464358No Hit
ATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAG18540.10509548724285901No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC18340.10396177109137186No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGAGATCG18290.10367834205350006No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC18100.10260131170958728No Hit
AGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGC18070.10243125428686421No Hit
GGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGA18040.10226119686414113No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG18010.10209113944141805No Hit
GTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTG17960.10180771040354626No Hit
TTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTC17910.10152428136567448No Hit
ATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCAC17710.10039056521418734No Hit
GCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTC17700.10033387940661295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTT150.002222776670.01845647
TTACGCG150.00222527769.9986143
GGGACTC150.002225527369.9966340
TGGTATA150.002225777469.99464441
CTAGTGG352.971483E-760.0158248
GTTTGAT5550.057.3829961
TGGTCCC509.822543E-1056.00047770
CACGAGG252.3922932E-455.99571641
TCTAGGG200.00694540552.51384444
TACTAGT407.4730633E-752.51384446
GACGCTA200.00695007952.50491367
CTAGCTT200.006951637652.50193462
AGTGAGT604.9312803E-946.6789747
ACGCGCT305.872626E-446.6789745
CGGTTGC305.8783736E-446.6697135
TTAGCCA305.880838E-446.6657443
CCTCTTA11750.046.166683
TGATCCT7000.044.995284
CTCTTAA12600.044.1620374
TCTTAAC12700.043.81435
Produced by FastQC (version 0.11.2)