FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n02_0at2a_prelim.35200000086ddb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n02_0at2a_prelim.35200000086ddb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3019074
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG494121.6366607774436797No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA380411.260022112740529No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG197540.6543065853967144No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC152290.5044261916070953No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT144210.4776630185281977No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT134520.4455670844768959No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG130190.43122493850763516No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT116090.38452187657540027No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT115140.3813752163742923No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT110220.3650788288064486No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA103440.342621611792225No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC103140.34162792962345406No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA91750.30390112994911683No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT87540.2899564568473645No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT87200.2888302837227574No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC82360.272798878066586No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG79990.26494878893329543No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT78860.2612059194309248No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG78020.25842360935836617No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT77430.2564693677597833No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG76310.2527596209963717No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT75780.25100411583154303No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT73410.24315402669825248No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC72080.23874870241670126No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG71290.23613200603893775No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT70020.2319254181911407No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG69830.23129608615091915No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT68450.2267251481745727No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC67580.22384346988513695No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA66910.22162424637488182No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC64910.21499969858307547No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG63840.21145556551445907No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA62520.20708336397186689No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG62090.20565908619662848No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA62000.20536098154599722No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC61880.20496350867848884No Hit
CTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAAGTG58780.19469545960118897No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC58450.19360240921554092No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT58350.1932711818259506No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA58270.19300619991427836No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT57600.19078697640402323No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC56970.18870024384960424No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT56270.18638165212247199No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT56210.18618291568871778No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT55840.1849573743472336No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT55100.18250629166426527No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG54440.18032019089296916No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG54090.17916089502940305No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG53290.1765110759126805No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT51840.1717082787636209No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT49780.16488499453806035No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA48810.16167208885903428No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC47200.15633932788663013No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA46790.15498129558930984No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG46770.15491505011139178No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC46440.15382199972574373No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA46130.15279519481801374No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA45800.15170214443236568No Hit
GTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAGGATGTTGGCTT45760.15156965347652956No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA45750.15153653073757054No Hit
ATCCCCTCCACCGAATGCCTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCA45530.15080783048047183No Hit
CTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTAGTCGGGACCTAA45280.14997976200649604No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA44890.14868797518709378No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC44180.14633626072100253No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC44000.14574005141973997No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT43630.14451451007825578No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG43530.14418328268866548No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG43440.1438851780380342No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG43360.14362019612636193No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA43210.14312335504197646No Hit
CTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCG43120.14282525039134517No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC42900.14209655013424646No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA42480.14070539509796712No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT42370.1403410449694178No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT41910.1388173989773023No Hit
CTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTACCGGGTTACCGAA41030.13590259794890752No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGAGATCG40850.13530638864764494No Hit
GTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTG40840.1352732659086859No Hit
CTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTG40160.13302091965947174No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA39800.1318285010569466No Hit
ATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCC39440.13063608245442146No Hit
GTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAA39360.1303711005427492No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG39220.12990738219732276No Hit
CTCCTCTTCTGCACTCAAGTTCCCCAGTTTCCAATGACCCTCCCCGGTTG39120.12957615480773244No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC38870.12874808633375665No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG38850.1286818408558386No Hit
GTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG38720.12825124524937118No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG38250.12669447651829668No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG38080.12613138995599313No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA38010.12589953078327992No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG37950.12570079434952572No Hit
CTCCGAAATAGCTTTAGGGCTAGCCTCAAGGTAAGAGTCTTGGAGGTAGA37870.12543581243785346No Hit
GTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAA37690.1248396031365909No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG37020.12262037962633575No Hit
CCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAG37010.12258725688737673No Hit
CGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCT36950.12238852045362253No Hit
CTCAAGGTAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCT36790.12185855663027802No Hit
GGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATT36780.121825433891319No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT36640.12136171554589253No Hit
ATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAA36380.12050052433295773No Hit
GTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCG36340.1203680333771216No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT35860.11877814190708806No Hit
GTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTAC35440.11738698687080873No Hit
CTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAT35280.11685702304746423No Hit
CTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGAAATTC34780.11520088609951262No Hit
ATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCAC34540.11440594036449586No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC34430.11404159023594652No Hit
GCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCT34310.11364411736843813No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA34150.11311415354509362No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGAC33990.1125841897217491No Hit
GTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTTGCTTACAGCTCC33940.11241857602695396No Hit
CTAAGGTCCCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAG33920.11235233054903589No Hit
CAGGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTA33100.1096362659543953No Hit
CAATGACTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG33070.10953689773751818No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC32780.10857633830770627No Hit
CTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCCTCAAGGTAAGAGTCTTG32700.10831135639603401No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA32630.10807949722332079No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTGATCCCGTTTCGCT32410.1073507969662221No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC32140.10645648301432822No Hit
ATCCTTTTCCACTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGC32030.10609213288577889No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT31550.10450224141574536No Hit
GTGAGAATGCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACC31440.104137891287196No Hit
CTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGT31370.10390603211448278No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA31150.10317733185738409No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC31080.10294547268467086No Hit
GGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCT30940.10248175433924442No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG30840.1021505269496541No Hit
CTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTG30810.102051158732777No Hit
ATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGC30790.10198491325485895No Hit
GGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTAC30770.10191866777694088No Hit
CCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGC30600.10135558121463734No Hit
GTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGACC30260.10022940809003024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAT509.822543E-1056.0093849
CACTAGT509.840733E-1055.9991843
GTTTGAT4350.054.7100261
TACTGAG200.006954896352.4966260
TGATCCT4250.051.879874
TCCTATT1500.048.9976582
GTCGCAT651.8553692E-1048.459221
GTCTCAT305.876078E-446.67448449
GCGAGTA604.9385562E-946.67448448
TGGTCCC1600.045.9391170
TCCTGGC4850.045.4617427
GTCCTTT2500.044.7978551
AGTGAGT1150.042.61583347
CTCTTAT1500.041.9966058
GTCCTCG855.638867E-1141.1745031
GTCTCTT2150.040.695731
CACTAGC701.9303116E-839.99941343
GCTAACC1050.039.998095
TCCTCGA801.4279067E-939.373122
TCTCTCT1900.038.682365
Produced by FastQC (version 0.11.2)