FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n01_79t2b_prelim.35100000086e3d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n01_79t2b_prelim.35100000086e3d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190351
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA403941.844179311900239No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG315941.442417219888502No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC245331.120048795832266No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG225811.0309306590587537No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT223121.0186495223824856No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT163670.7472318363586475No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG142700.651493756023578No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA125290.5720087785017105No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA96600.4410252055492476No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG93910.4287440688729797No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT88520.4041361407372608No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA81760.3732735073054501No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT80120.3657861228634132No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT78780.35966838191687084No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT78120.35665516622678284No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG76200.34788944785561765No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT75090.3428217669222878No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG75060.34268480257273837No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG71490.3263860449763531No Hit
GGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAG69050.315246277879664No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT68190.311319966525913No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA63610.29041007582802936No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT61740.28187263137278No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA60680.27703322435536587No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT60390.2757092356430545No Hit
CGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAA60240.2750244138953072No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA54610.24932077096319263No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC54010.24658148397220356No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCA52820.24114856477340849No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT52280.23868320648151825No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA52240.23850058734878565No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA51350.23443731164548512No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA51350.23443731164548512No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG51070.2331589777163569No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG50450.23032838115900145No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA48110.21964516189414393No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC47940.21886903058003032No Hit
CTGTGTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCG47200.21549057662447707No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT47190.21544492184129393No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA47050.21480575487672982No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT46870.21398396877943307No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT46810.21371004008033417No Hit
GACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATC46530.2124317061512059No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT46170.21078813395661242No Hit
CGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAG45140.20608569128874776No Hit
ATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATG44460.20298116603229346No Hit
GACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAG43180.19713735378484998No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG42700.19494592419205872No Hit
CTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAAGTG42490.19398717374521254No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC42440.19375889982929675No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT41150.18786943279867016No Hit
TGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATAT40830.1864084797368093No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC40740.18599758668816094No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG40580.1852671101572305No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT40550.18513014580768106No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT40500.1849018718917653No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG39420.17997115530798488No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC39180.17887544051158924No Hit
CTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAG38570.17609049873741697No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT37480.1711141273704534No Hit
GAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCTC37250.17006406735724092No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT36830.16814656646354853No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG36050.16458549337526268No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTGATCCCGTTTCGCT35990.16431156467616378No Hit
CTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTAGTCGGGACCTAA35140.16043090810559585No Hit
CGGGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTAC34300.1565959063182111No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG34020.15531757238908286No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT33790.15426751237587036No Hit
CGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGA33710.15390227411040514No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC33680.15376530976085567No Hit
TTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTC32670.14915417665935735No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT32460.14819542621251114No Hit
GTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCG31560.14408649572602747No Hit
CTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCGCCTACCTG31420.14344732876146335No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT31350.1431277452791813No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC31050.14175810178368672No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG30940.14125589916867207No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT30930.1412102443854889No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC30900.14107328003593944No Hit
CTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTG30260.13815137391221774No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT30140.1376035165140199No Hit
GCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGT30070.13728393303173783No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT29640.1353207773548623No Hit
CTCATACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTG29560.1349555390893971No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT29080.13276410949660578No Hit
CTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT29030.13253583558069004No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA29020.13249018079750688No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA28830.131622739917027No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG28760.13130315643474494No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT28690.13098357295246288No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG28620.13066398947018082No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA28520.1302074416383493No Hit
CTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATA28280.12911172684195366No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG27940.12755946421372646No Hit
CCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTG27300.12463755809000475No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG27130.12386142677589117No Hit
ATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCT26960.12308529546177759No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC26880.12272005719631236No Hit
CTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAGAGT26680.12180696153264933No Hit
GGGAAACCCACCACTCGTAATGGAGTGGTATCCATATCTGAATTCATAGG26460.12080255630261998No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC26420.12061993716988738No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT26390.12048297282033793No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA26340.12025469890442216No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA26320.12016338933805587No Hit
CTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGT26300.12007207977168956No Hit
CTTGGTTTGGGCTGATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT26210.1196611867230412No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA26040.11888505540892762No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA25820.11788065017889826No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA25730.1174697571302499No Hit
CAAGAGTCCACATCGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCG25720.11742410234706674No Hit
CGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGGGCTG25430.11610011363475535No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC25420.1160544588515722No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG25320.11559791101974067No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC25210.11509570840472601No Hit
AGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGC25190.11500439883835968No Hit
GGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTAC24990.11409130317469665No Hit
GGAAGAGAAAGCAAATGCGATTCCCTGAGTAGCGGCGAGCGAAACGGGAT24900.1136804101260483No Hit
GTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCT24740.11294993359511785No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC24540.11203683793145482No Hit
ATTGAATGTTGGTACAGCTTGTACAGGATAGGTAGGAGCCTTGGAAACCG23920.10920624137409939No Hit
CGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCG23830.10879534832545104No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT23690.10815618136088691No Hit
CCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTTG23400.10683219264857549No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG23260.10619302568401139No Hit
ATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAA23150.1056908230689967No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA22970.10486903697169997No Hit
TTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCT22860.10436683435668528No Hit
CTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCTTCT22800.10409290565758639No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA22680.10354504825938857No Hit
GTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCGGGCT22530.10286022651164127No Hit
ATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGGGTTTCCCCAT22520.10281457172845813No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA22420.10235802389662661No Hit
CTTTACTGCAGCCTGATATTGAATGTTGGTACAGCTTGTACAGGATAGGT22110.10094272561794891No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT207.925862E-570.007771
CTATAAG150.002225202569.9997711
GACTAAT150.002225202569.9997712
AGATATA1000.066.4997869
TTTGGGG750.065.3331212
CCCTATG1450.065.17219514
CTAGGCG550.063.6361588
GGTCCAC501.4551915E-1162.99979410
CACGACC501.4551915E-1162.99979414
CCGTGTA453.8562575E-1062.2220157
GATCCAG1250.061.59979670
TGATCCA1350.059.62943669
GACACTA308.3827745E-658.33314512
GTTTGAT3600.056.395141
CGGGAGT252.3902074E-456.0062071
ATGAGAC252.3915558E-455.99981769
CCTCTGG1000.055.99981710
GATATAG1200.055.4164970
CCTATAA700.054.9998258
CTAGCTG452.7028364E-854.450481
Produced by FastQC (version 0.11.2)