FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n01_79t2a_prelim.35100000086df8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n01_79t2a_prelim.35100000086df8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735705
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG429731.570819953174776No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA285581.0438991046183708No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT246280.9002432645332739No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG190310.6956524917708597No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT164430.6010516484781802No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG148750.5437355270396479No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT116650.42639831414571383No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC111010.40578205617930296No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT101590.3713485189375316No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT96150.3514633339486531No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT88720.3243039728333281No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT87010.318053298875427No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC85770.3135206464147267No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA78420.2866537144904147No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC78320.2862881780016486No Hit
CTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTAGTCGGGACCTAA77850.28457015650444767No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA77480.2832176714960129No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT77230.28230383027409756No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG76520.2797085212038579No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC75920.277515302271261No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG75860.2772959803780013No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG74810.27345784724595673No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA74680.2729826498105607No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA74500.27232468413078165No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC68730.2512332287289748No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC68240.24944209993402067No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT66630.24355696246488565No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT64170.23456476484123834No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC63050.23047075616705748No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT62190.2273271423636686No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT60850.22242895341420219No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG60560.22136889759678036No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG59220.21647070864731394No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA57870.21153596604897093No Hit
ATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAA57730.2110242149646983No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA57240.20923308616974415No Hit
CTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGAAATTC55700.20360382424274548No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC55020.20111817611913563No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG54950.20086230057699936No Hit
CTAAGGTCCCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAG54650.1997656911107009No Hit
CTCAAGGTAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCT53780.1965855236584354No Hit
CTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTACCGGGTTACCGAA53310.1948675021612345No Hit
CTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCCTCAAGGTAAGAGTCTTG51900.18971343766963178No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA51050.1866063775151195No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA50590.1849249096667952No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC49920.18247581519206202No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT49680.18159852761902326No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT49610.18134265207688693No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG48900.17874734300664727No Hit
ATCCCCTCCACCGAATGCCTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCA48660.17787005543360854No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG48540.17743141164708914No Hit
CTCCTACCTATCCTGTACAAGCTGTACCAACATTCAATATCAGGCTGCAG48490.17724864340270605No Hit
ATCCTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACC47510.1736663858127978No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT47320.17297186648414212No Hit
CTCCGAAATAGCTTTAGGGCTAGCCTCAAGGTAAGAGTCTTGGAGGTAGA47070.17205802526222674No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTGATCCCGTTTCGCT46970.1716924887734606No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA46890.17140005958244767No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC46040.1682929994279354No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT45980.1680736775346757No Hit
ATCCTTTTCCACTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGC45960.16800057023692247No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT44940.16427209805150775No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT44550.16284650574531975No Hit
CTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTG44150.16138435979025517No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT43280.15820419233798966No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA43090.15750967300933397No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA42490.15531645407673705No Hit
GGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCT42320.15469504204583462No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA41490.1516610891890756No Hit
GTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAGGATGTTGGCTT41310.1510031235092965No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA40360.14753052686601809No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT40170.1468360075373624No Hit
CTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCG40130.14668979294185594No Hit
GTCAGAGCCCGTTAACGGGTGATGGCGTGCCTTTTGTAGAATGAACCGGC39970.1461049345598301No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG39930.14595871996432364No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT39570.1446427886047655No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG39540.14453312765813567No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG39490.14435035941375257No Hit
ATAAGATCCGTAGTCGAAAGGGAAACAGCCCAGACCGCCAGCTAAGGTCC39280.14358273278734368No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG39000.14255923061879844No Hit
CTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGT38910.1422302477789089No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG38900.1421936941300323No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG38190.13959838505979263No Hit
CTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCTTCT38010.13894041938001356No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA37880.13846522194461758No Hit
CAATGACTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG37780.1380996854558514No Hit
CTAAAAGGTAACGGAGGCGCCCAAAGGTTCCCTCAGAATGGTTGGAAATC37740.13795347086034496No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG37570.1373320588294425No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG37450.13689341504292313No Hit
ATCGCATTTGCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCG37280.13627200301202066No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA37270.13623544936314405No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG36900.13488296435470928No Hit
CCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAG36660.13400567678167055No Hit
CTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTC36660.13400567678167055No Hit
CTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCGAAAGGG36570.133676693941781No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT36530.13353047934627454No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC36480.13334771110189148No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC36420.13312838920863176No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT36100.1319586724445801No Hit
ATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCAC35900.13122759946704782No Hit
GGCAAATCCGTTTCCCATAAGGCTGAGCTGTGATGGCGAGCGAAATATAG35730.13060618743614535No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG35110.1283398612057952No Hit
CTCATACCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTG34900.1275722345793863No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC34660.12669494700634754No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT34580.12640251781533463No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC34450.12592732037993862No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC34440.125890766731062No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA34120.12472104996701033No Hit
ATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGC33950.12409963793610788No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT33730.12329545766082235No Hit
CTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTG32960.12048082669732299No Hit
CTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAAGTG32960.12048082669732299No Hit
ATTGGACTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAAT32800.11989596831529716No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC32750.11971320007091407No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT32710.11956698547540762No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA32520.11887246614675194No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT31930.11671580086303164No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG31910.11664269356527841No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCA31780.11616749612988243No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG31710.11591162058774611No Hit
GTCTTTCCGTCCTGTCGCGGGTAACCTGCATCTTCACAGGTACTATAATT31450.11496122571695412No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG31410.11481501112144768No Hit
CTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAT31350.11459568922818797No Hit
CTGTGTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCG31090.11364529435739598No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA30960.113170096922No Hit
CTGAATTCATAGGATATGAGAAGGCAGACCCGGGGAACTGAAACATCTAA30920.11302388232649353No Hit
ATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCC30810.11262179218885077No Hit
GTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGAC30770.11247557759334433No Hit
ATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATG30340.11090377069164986No Hit
GTGGATCCTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAG30130.11013614406524094No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT29870.10918574919444896No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC29620.1082719079725336No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC29520.10790637148376744No Hit
CTTTTCCACTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGCTGT29480.10776015688826097No Hit
CTTAGACAACCAGGATGTTGGCTTAGAAGCAGCCACCATTTAAAGAGTGC29340.10724840580398837No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT29260.10695597661297546No Hit
CTCCTACCACTGTTCGAAGAACAGTCCGCAGCTTCGGTGATACGTTTAGC29250.10691942296409884No Hit
CTTTACTGCAGCCTGATATTGAATGTTGGTACAGCTTGTACAGGATAGGT28760.1051282941691447No Hit
CTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAG28690.1048724186270084No Hit
CTCGTAATGGAGTGGTATCCATATCTGAATTCATAGGATATGAGAAGGCA28680.10483586497813178No Hit
GTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGCGGAGTG28470.10406823835172287No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA28430.10392202375621641No Hit
CTGCAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCA28330.10355648726745026No Hit
CTAGCCTCAAGGTAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGG27940.10213089496126228No Hit
CGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCT27650.10107083914384044No Hit
ATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCTTCTCC27500.1005225344106912No Hit
GCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAA27490.1004859807618146No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCC1000.062.99973310
CTAGGCG501.4551915E-1162.9997338
ACACTAG453.8562575E-1062.22196613
TCGACGC308.383378E-658.33308810
TGGGTAC850.057.6468214
ACTAGGC553.6379788E-1157.2724887
ACTATCA252.391672E-455.99976369
GTCTCGA252.391672E-455.99976311
TTGACAC509.840733E-1055.99976310
CCCTATG1150.054.7823814
TGCGACT452.7055648E-854.444229
TCTGGGT850.053.5291912
GCCCACG200.00695317152.49978311
GCGGTAG200.00695317152.49978313
GACTTAC200.00695317152.49978346
ACCTAGT200.00695317152.4997839
CTAAACT200.00695317152.49978313
CACACTA407.4884156E-752.49978369
TAGTCTA200.00695317152.4997839
CCTCTGG950.051.57873510
Produced by FastQC (version 0.11.2)