FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n01_79t0b_prelim.35100000086e20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n01_79t0b_prelim.35100000086e20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1819156
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA196191.0784671572971203No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC180870.994252279628575No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG145310.7987770152752155No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG144540.7945442831730759No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT128120.7042826453586168No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT108270.5951661099982629No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA72700.39963587509812243No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT71600.3935891149522086No Hit
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG69620.38270494668956373No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT65220.35851790610590845No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT61030.33548524700465493No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA58710.3227320801514548No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA58590.3220724335900824No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA55640.3058561222896772No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT53890.2962362766029961No Hit
GACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAG52410.28810063567940297No Hit
GGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAG51140.2811193762382116No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG50650.27842581944594086No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT48990.2693007086802891No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA48460.26638726970089427No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC47970.26369371290862353No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG47400.2605603917421046No Hit
GACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATC47290.2599557157275132No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT42910.23587861623741999No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG41440.22779794586060786No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCA40990.22532427125546134No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC40290.22147633298078892No Hit
CTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATA40260.22131142134044576No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT39220.21559448447521817No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG38910.2138903975250061No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT38300.210537194171363No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG37380.20547990386750778No Hit
GGGAAACCCACCACTCGTAATGGAGTGGTATCCATATCTGAATTCATAGG37200.2044904340254492No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA36700.20174190668639744No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA36390.20003781973618537No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA35790.19673958692932328No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG35250.1937711774031474No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT35180.19338638357568017No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT33990.186844888508737No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT33900.1863501535877077No Hit
CGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAA33310.18310689132762667No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA32740.17997357016110768No Hit
TTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTC32720.1798636290675456No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT32690.1796987174272025No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT32600.1792039825061732No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC32050.1761806024332163No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT31090.17090342994223695No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG29840.1640321115946076No Hit
GAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCAC29360.16139352534911794No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT29360.16139352534911794No Hit
GGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTAGG28760.15809529254225585No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT28700.15776546926156965No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA28500.15666605832594896No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG27880.15325788442552482No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC27670.15210350294312308No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA27660.15204853239634203No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA27320.15017953380578686No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG26780.147211124279611No Hit
GGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTAC26720.14688130099892477No Hit
GCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGT26630.14638656607789546No Hit
CAGGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTA26230.1441877442066541No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG25890.1423187456160989No Hit
GGAAGAGAAAGCAAATGCGATTCCCTGAGTAGCGGCGAGCGAAACGGGAT25890.1423187456160989No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT25490.1401199237448575No Hit
GTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGACC25130.13814098406074027No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA24940.1370965436719006No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA24890.13682169093799543No Hit
GAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCTC24600.1352275450813454No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG24590.13517257453456438No Hit
ATCGCATTTGCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCG24520.13478778070709713No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG24280.13346848758435229No Hit
CGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAG24100.13247901774229368No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC23880.1312696657131109No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG23640.12995037259036607No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT23550.12945563766933676No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT23420.12874102056118333No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG23190.12747669798521952No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC23080.12687202197062813No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC22990.12637728704959883No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG22950.1261574048624747No Hit
TTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCT22760.125112964473635No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC22590.12417846517835743No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT22260.12236443713458328No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT22190.12197964330711605No Hit
CTGGTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCT22000.12093520291827639No Hit
GGACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGT21830.12000070362299879No Hit
CTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGATAAGTC21640.11895626323415913No Hit
CTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAAT21610.11879135159381603No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT21550.11846152831312984No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT20850.11461359003845739No Hit
GTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCG20840.11455861949167635No Hit
GGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAAC20790.11428376675777119No Hit
GGGAAATCTCATCTTGAGGGGGGCTTCATGCTTAGATGCTTTCAGCACTT20700.11378903183674187No Hit
CGGGCCTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTAC20570.11307441472858841No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC20470.11252470926077808No Hit
GTGGGATACTACCCTGGCTGTATTGACCTTCTAACCCGCCGCCCTTATCG20430.11230482707365393No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA20420.11224985652687289No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT20270.11142529832515738No Hit
CGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAATGGTGGCT20130.11065571067022288No Hit
ATAAGATCCGTAGTCGAAAGGGAAACAGCCCAGACCGCCAGCTAAGGTCC19990.10988612301528841No Hit
CTTTCCAGACCTCTTCATCTACCTCGTTCCTTTGTAACTCCGTACAGAGT19760.10862180043932461No Hit
CCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTG19760.10862180043932461No Hit
GTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAA19720.10840191825220047No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG19660.10807209497151425No Hit
CTTAGATGCTTTCAGCACTTATCCCGTCCGCACATAGCTACCCAGCGATG19580.10763233059726598No Hit
CTTGGTTTGGGCTGATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT19340.10631303747452114No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT19340.10631303747452114No Hit
ATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAG19290.10603818474061598No Hit
GGAAATTTGAGAGGAGCTGTCCTTAGTACGAGAGGACCGGGATGGACGCA18980.1043340977904039No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC18970.10427912724362287No Hit
GGCACCTCGATGTCGGCTCATCGCATCCTGGGGCTGTAGTCGGTCCCAAG18930.10405924505649873No Hit
GGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGA18790.10328965740156425No Hit
CGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGT18640.10246509919984872No Hit
AGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGC18550.1019703642788194No Hit
ATTGAATGTTGGTACAGCTTGTACAGGATAGGTAGGAGCCTTGGAAACCG18510.10175048209169527No Hit
GCGAAATATAGTAGCGAAGTTCCTGATTCCACACTGCCAAGAAAAGCCTC18490.1016405409981332No Hit
CGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACC18430.10131071771744699No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA18400.10114580607710388No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA18320.10070604170285562No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG207.930228E-569.9993413
CGCTATA308.382514E-658.33278311
CTAGGCG308.382514E-658.3327838
CATACCC1000.055.99947710
TATGGAG200.00695294552.49950412
CCCGTGA200.00695294552.49950469
GGCTCTA200.00695294552.49950411
CTGGCAT750.051.349791
GCCGGGT1150.048.711261
TCCTATT751.4551915E-1146.6816252
GCGGGAT751.4551915E-1146.6816251
GCTATAG305.8805844E-446.66622512
ACTAGGC305.8805844E-446.6662257
GGCGCAC1600.045.952221
CTCCGGC703.8562575E-1044.99957769
CCAATTG703.8562575E-1044.9995779
TCCTATA703.8562575E-1044.9995777
GGTCCAC404.5961246E-543.74958810
CCCTATG1300.043.07651514
CCTATAA651.0031727E-843.0765158
Produced by FastQC (version 0.11.2)