FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n01_79t0a_prelim.35100000086deb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n01_79t0a_prelim.35100000086deb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2475101
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG246530.9960401615934056No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA133500.5393719286606891No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG120100.48523272383632027No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC94820.3830954777198991No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT85300.3446324008596013No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT74920.30269471831654543No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT72370.2923921084432514No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC71270.287947845360654No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC70370.2843116301112561No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG69630.2813218531284178No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT66680.2694031475887247No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA63120.25501989615777293No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT63080.2548582865911331No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT60970.24633338195087798No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT60110.24285877626811997No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG55330.2235464330546511No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT53410.2157891738559356No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC52300.21130450838167816No Hit
GTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG51500.20807231704888002No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT51440.20782990269892018No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG51300.2072642692156805No Hit
CGGGATCGTAACTCGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGT50090.2023755798248233No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA49390.19954741240862492No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG49320.19926459566700513No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT47290.19106291016002983No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC47220.19078009341840999No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG46900.18948721688529074No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG45590.1841945035778338No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC45260.18286122465305457No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG44370.17926541179531663No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT43980.17768971852057752No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT43400.1753463798042989No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT42210.17053849519676167No Hit
GACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATC42200.1704980928051017No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA42190.1704576904134417No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT41620.16815475408882305No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA41520.16775073017222328No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT40740.1645993436227451No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT40570.1639125029645255No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG40230.16253882164808628No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC40090.16197318816484663No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA39450.15938743509860812No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT39230.15849858248208862No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG39220.15845818009042864No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA38980.1574885226905892No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT38910.15720570594896935No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA38880.15708449877398942No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG38870.15704409638232944No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC38620.15603403659083004No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA37590.15187259024985245No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA36620.1479535582588347No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA35840.14480217170935653No Hit
ATCGCATTTGCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCG35310.14266084495137774No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT34750.14039831101841904No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG34010.13740853403558076No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC33950.1371661196856209No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG33880.13688330294400108No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC33820.13664088859404122No Hit
GACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAG32800.13251984464472358No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA32730.13223702790310374No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC32430.13102495615330445No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG31350.12666149785402697No Hit
CAGGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTA31050.12544942610422766No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG30970.12512620697094787No Hit
CCGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTC30930.12496459740430794No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT30850.12464137827102814No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG30790.12439896392106826No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT30710.12407574478778846No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG30390.12278286825466919No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA29250.11817699560543186No Hit
GGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTC29220.11805578843045192No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT29180.11789417886381202No Hit
GAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTG29050.11736894777223232No Hit
GGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATT28890.1167225095056727No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG28710.11599526645579311No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG28680.1158740592808132No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC28360.11458118274769392No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT28220.11401554926445427No Hit
GGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCA27960.11296508708129487No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGAC27900.112722672731335No Hit
GTGAACCAGTACCGTGAGGGAAAGGTGAAAAGCACCCCGGAAGGGGAGTG27760.11215703924809532No Hit
GGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAAC27740.11207623446477537No Hit
GCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCT27340.11046013879837631No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC26920.10876323834865728No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC26640.10763197138217794No Hit
CCCAAGGCTAAATACTCCCTAGTGACCGATAGTGAACCAGTACCGTGAGG26350.10646030202403861No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA26290.10621788767407876No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG26260.10609668049909882No Hit
GGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTAC26030.10516742549091937No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT26000.10504621831593944No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT25870.10452098722435973No Hit
CGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATC25840.1043997800493798No Hit
GGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGG25800.1042381704827399No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA25570.10330891547456045No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT25220.10189483176646125No Hit
GCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACCGAATGCCT25000.10100597914994176No Hit
CAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGA24970.10088477197496182No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA24880.10052115045002205No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA24760.10003632175010232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACCC900.058.3332510
GACACTA252.3915948E-455.9999212
ACACTAG252.3915948E-455.9999213
CCCTATG950.055.2630814
TACTAAT506.205482E-848.9999363
GGCGCAC2800.048.7528881
CTCCCTC1900.047.89467214
TCTCCCT1900.047.89467213
TCCTGGC1250.047.599937
CCACGGT451.6859012E-646.669431
ACTAATC551.3134559E-744.54539564
CTAGGCG551.3134559E-744.5453958
TCTTATT1350.044.0740139
CTGGCAT404.594745E-543.752591
GGCCCAC651.00335455E-843.076868
CTCTCCC2250.041.99994312
GCCGGGT1100.041.3660851
ACTAGGC602.6014823E-740.833277
GCGCACG3400.040.1486242
GTCTAAC350.001255683740.002371
Produced by FastQC (version 0.11.2)