FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n01_0at2a_prelim.35100000086dde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n01_0at2a_prelim.35100000086dde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3019074
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG406741.3472342844196599No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA367741.2180556024794358No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG202530.6708348321372712No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC153950.5099245662742947No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT147020.48697050817568566No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT132700.4395387459863521No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG131320.4349678080100057No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT111270.36855671639714693No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA104930.3475568998971208No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC103400.3424891208363889No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC103050.3413298249728228No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT102130.3382825329885919No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT101520.33626204591209097No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT99070.3281469748671281No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA91670.3036361480374446No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG87060.28836656537733096No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT81660.27048028633945376No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG81410.26965221786547794No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG79790.2642863341541148No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT76840.2545151261612004No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT76140.2521965344340682No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT74820.24782433289147598No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT74100.2454394956864257No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC72960.24166350344509607No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC72370.2397092618465132No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA70720.23424400991827296No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT69620.23060050863277945No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG68630.2273213574758353No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG68290.2261951843512282No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT66890.22155800089696376No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG66530.22036558229443864No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA65300.21629148540247772No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG65260.21615899444664158No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT64590.21393977093638644No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA64000.21198552933780357No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG63780.21125682908070487No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG63160.20920321926524488No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC60370.199961975095675No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA60010.1987695564931499No Hit
CGAATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGAC59870.19830583814772346No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT58860.1949604415128612No Hit
CTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAAGTG58830.19486107329598415No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT58460.19363553195449995No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT57140.18926333041190774No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC56710.1878390526366694No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT56060.1856860746043323No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT56040.18561982912641425No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA52990.17551739374390957No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT51880.17184076971945703No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC49870.16518309918869162No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG49800.1649512400159784No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA48860.16183770255382943No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC48380.16024781108379588No Hit
CTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACCATC47920.1587241650916804No Hit
ATCCCCTCCACCGAATGCCTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCA47490.15729988731644207No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGGGATCG47280.1566043097983024No Hit
CTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAG46940.1554781366736953No Hit
CTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTAGTCGGGACCTAA46690.15465006819971952No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA46380.15362326329198953No Hit
GTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAGGATGTTGGCTT46230.15312642220760406No Hit
GTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACG46210.153060176729686No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC46180.1529608085128089No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA45640.15117218060902116No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC45400.15037723487400442No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA44810.14842299327542152No Hit
CTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAA44520.14746243384560961No Hit
CTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTC44380.14699871550018317No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG43920.1454750695080677No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG43570.1443157736445016No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC43350.1435870733874029No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG43240.14322272325885355No Hit
GTCATTTTGCCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATT42700.14143409535506582No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT42410.1404735359252539No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT42390.14040729044733583No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA42030.13921487184481068No Hit
CGCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGA41750.1382874351539578No Hit
GTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCG41570.13769122585269522No Hit
CCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACT41470.1373599984631049No Hit
CGCAGCTTCGGTGATACGTTTAGCCCCGGTACATTTTCGGCGCAGAGTCA41440.13726063024622784No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG40910.1355051250813991No Hit
GTCCATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTG40690.13477642482430044No Hit
CTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGAAATTC39660.13136478271152013No Hit
GTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAA39610.131199169016725No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA38990.12914555920126503No Hit
ATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCC38870.12874808633375665No Hit
CTCGCCATCACAGCTCAGCCTTATGGGAAACGGATTTGCCTATTTCCCAG38090.12616451269495216No Hit
CCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAGACAACCAG38040.12599889900015698No Hit
CTCCGAAATAGCTTTAGGGCTAGCCTCAAGGTAAGAGTCTTGGAGGTAGA37920.12560142613264863No Hit
CTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCTACCGGGTTACCGAA37890.12550205791577151No Hit
GTCCAATCAGTGCTCTACCTCCAAGACTCTTACCTTGAGGCTAGCCCTAA36950.12238852045362253No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG36920.12228915223674544No Hit
CCGAGTTCCTTAACGAGAGTTCTCTCGATCACCTTAGGATTCTCTCCTCG36550.12106361089526126No Hit
CTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAGATCACCTG36270.12013617420440836No Hit
CAATGACTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCG36250.1200699287264903No Hit
CTGACTACTTGTAGGCACACGGTTTCAGGATCTCTTTCACTCCCCTTCCG36240.12003680598753126No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT36190.11987119229273611No Hit
ATCCTGAGTACGGCGGAACACGTGAAATTCCGTCGGAATCCGGGAGGACC36010.11927498299147353No Hit
CTCCTCTTCTGCACTCAAGTTCCCCAGTTTCCAATGACCCTCCCCGGTTG35930.11901000107980128No Hit
CTAGGGGCCCCTACCGGGTTACCGAATTCAGTCAAACTCCGAATGCCAAT35820.11864565095125194No Hit
CAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGTGA35770.1184800372564568No Hit
GTGGGTTTCCCCATTCGGAAATCTCCGGATCAAAGCTTGCTTACAGCTCC35740.1183806690395797No Hit
ATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAA35550.11775133699935808No Hit
CTCAAGGTAAGAGTCTTGGAGGTAGAGCACTGATTGGACTAGGGGCCCCT35100.11626081374620165No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT35070.11616144552932456No Hit
CTAAGGTCCCAAAGTATACGTTAAGTGGAAAAGGATGTGGAGTTGCTTAG34930.1156977271838981No Hit
GTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTCCCTAGTGACC34830.1153664997943078No Hit
CGTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCT34830.1153664997943078No Hit
CGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCA34820.11533337705534877No Hit
GTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTAC34600.11460467679825007No Hit
GTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGGGTAG34490.11424032666970071No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC34430.11404159023594652No Hit
CTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCACGAGATCG34300.1136109946294791No Hit
CTGAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGA33830.1120542258984046No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA33160.10983500238814947No Hit
CTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGT33120.10970251143231335No Hit
CTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCCTCAAGGTAAGAGTCTTG32640.10811261996227983No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC32570.10788076078956661No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG32530.10774826983373047No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC32480.10758265613893532No Hit
CTTATCCTTGGGAGTCAGACTGCGAGTGATAAGATCCGTAGTCGAAAGGG32360.10718518327142693No Hit
ATTCAGTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGC32230.10675458766495952No Hit
GTCAGAGCCCGTTAACGGGTGATGGCGTGCCTTTTGTAGAATGAACCGGC32210.10668834218704144No Hit
GGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCT31730.10509845071700794No Hit
GGTAGATCACCTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATT31640.10480034606637666No Hit
GGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCGTAC31380.10393915485344182No Hit
CAGGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTA31310.10370729568072859No Hit
GTTCACTATCGGTCACTAGGGAGTATTTAGCCTTGGGAGATGGTCCTCCC31280.10360792746385149No Hit
CTTAGACAACCAGGATGTTGGCTTAGAAGCAGCCACCATTTAAAGAGTGC31250.10350855924697441No Hit
ATCCTTTTCCACTTAACGTATACTTTGGGACCTTAGCTGGCGGTCTGGGC31250.10350855924697441No Hit
GTGAGAATGCCGGTATGAGTAGCGAAAGAGGGGTGAGAATCCCCTCCACC30830.10211740421069507No Hit
ATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCTTGCAC30790.10198491325485895No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA30780.10195179051589992No Hit
GCCGGTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGAC30700.10168680860422766No Hit
CTCGGCTTAGGTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAA30480.10095810834712896No Hit
GCCCTATTCAGACTCGCTTTCGCTGCGGCTCCGCATCTTCTGCTTAACCT30440.10082561739129282No Hit
CCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCC30420.10075937191337475No Hit
GCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGT30420.10075937191337475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGG550.069.9998414
GTAGAGT150.002225276569.999838
TAGGCGC650.064.6152349
CATACCC850.061.76455710
CTAGGCG750.060.6665238
TGTGTGT352.9776675E-759.99985514
GTCCTCG308.379384E-658.3380281
GGCCTAA900.058.3380281
CGCGCCT252.390712E-456.004511
TGGGTAC509.840733E-1055.9998714
GGCCGGG252.3916904E-455.9998711
TGTACCT509.840733E-1055.9998710
CCCTATG950.055.26302714
CTATGTA407.4885793E-752.4998789
TGAGTCC407.4885793E-752.49987870
TAGCCAC200.006953192452.49987814
ACTAGGC800.052.4998787
GAGCCAC200.006953192452.49987870
ACTCCCG200.006953192452.49987810
CAACTCT200.006953192452.4998788
Produced by FastQC (version 0.11.2)