FastQCFastQC Report
Thu 6 Apr 2017
000000000-AUWYC_l01n01_0at0b_prelim.35100000086e06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AUWYC_l01n01_0at0b_prelim.35100000086e06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2296087
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACTAGGG261321.1381101848492674No Hit
CTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGA230661.0045786592581203No Hit
CTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGG188060.819045619787055No Hit
GTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATA120590.5251978692445016No Hit
CTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTT120260.5237606414739512No Hit
CACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCC115990.5051637851701613No Hit
GTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTT99500.43334594899931933No Hit
CCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCAC96940.422196545688382No Hit
GGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGAT92470.40272864225092514No Hit
CGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGAT84200.3667108432737958No Hit
GCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCT83590.36405414951611154No Hit
CTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGGTGG78600.34232152353112055No Hit
CCCCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCA73850.32163415410652996No Hit
CCGGTACATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACT70020.3049536014968074No Hit
GGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGAGCACCCCT67530.2941090646826536No Hit
GGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGA61690.26867448837957797No Hit
GAGCGTTCTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTT61060.265930689908527No Hit
CACGGTTTCAGGATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTT59830.2605737500364751No Hit
CCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCATTC59740.260181778826325No Hit
GTCCCGACTAACCCTGAGCGGACGAGCCTTCCTCAGGAAACCTTAGGCAT56210.2448077969171029No Hit
GGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATG56200.24476424456041954No Hit
GTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATC56140.24450293042031943No Hit
GTCGGTTTGCGGTACGGGCACCTCTCACCTCGCTAGAGGCTTTTCTTGGC55630.24228176022946865No Hit
CTGGTTTCGGGTCTACGACCACGTACTCATGCGCCCTATTCAGACTCGCT54730.23836204812796727No Hit
CCTCGATCGATTAGTATCTGTCAGCTCCATGTGTCGCCACACTTCCACCT50990.2220734667283949No Hit
GTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAAGCC50770.2211153148813612No Hit
GTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACC47480.20678658953253948No Hit
GTTCATTCTACAAAAGGCACGCCATCACCCGTTAACGGGCTCTGACTACT45850.19968755539315364No Hit
GACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATC45610.1986422988327533No Hit
CTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGTGG45030.19611626214511907No Hit
CAGGGATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCT44780.19502745322803536No Hit
GGCTGTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCA44750.1948967961579853No Hit
CTCGTTCCTTTGTAACTCCGTACAGAGTGTCCTACAACCCCAAGAGGCAA44430.19350312074411816No Hit
GATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATATGC44200.19250141654040112No Hit
CTCCTCTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCA44110.19210944533025098No Hit
CAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATG43920.19128195055326738No Hit
CGGAGATTTCCGAATGGGGAAACCCACCACTCGTAATGGAGTGGTATCCA43850.19097708405648392No Hit
CCGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCA43770.19062866520301713No Hit
CTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCA43760.19058511284633378No Hit
GAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAA42580.18544593475769863No Hit
CTAGGAGCCGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTG40370.17582086393067858No Hit
GGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGG39500.17203180889922726No Hit
CTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGTTCCGCCG39020.1699412957784265No Hit
CTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGGTGATGGCG38780.16889603921802615No Hit
ATCTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATG38210.16641355488707527No Hit
CAGCCCTGTAGTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGG38120.16602158367692513No Hit
GTGAAATGCCAATCGAACCTGGAGATAGCTGGTTCTCTCCGAAATAGCTT37900.16506343182989144No Hit
CGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGA37090.1615356909385402No Hit
GGACACTCTGTACGGAGTTACAAAGGAACGAGGTAGATGAAGAGGTCTGG36850.16049043437813987No Hit
GAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTAACAG36710.15988070138457297No Hit
GCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACTCGTAAT35930.15648361756327178No Hit
GAAAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACG34830.15169285832810342No Hit
GAAACATCTAAGTACCCGGAGGAAGAGAAAGCAAATGCGATTCCCTGAGT34490.15021207820086957No Hit
AAGAGATCCTGAAACCGTGTGCCTACAAGTAGTCAGAGCCCGTTAACGGG34460.15008142113081951No Hit
GTCAAAACTTCGTTCTCTCCTGAGTGGATCCTGAGTACGGCGGAACACGT34040.14825222215011888No Hit
CTTACTTCCTTGCGGAATGGGAAATCTCATCTTGAGGGGGGCTTCATGCT33240.144768033615451No Hit
CTAACTGCTTGGACGCGGATATCCAATACCGCGCTTACCCTATCCTCCTG33060.1439840911951507No Hit
GTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCGTGTGCC32760.14267752049465024No Hit
ATCGCATTTGCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCG32630.1421113398577667No Hit
CCCGACTTTCGTCCCTGCTCGACTTGTAGGTCTCGCAGTCAAGCTCCCTT32540.14171936864761658No Hit
GTCGCGGGTAACCTGCATCTTCACAGGTACTATAATTTCACCGAGTCTCT32530.14167581629093323No Hit
CTGCAGTAAAGCTCCACGGGGTCTTTCCGTCCTGTCGCGGGTAACCTGCA32530.14167581629093323No Hit
GTTTCCCTTTCGACTACGGATCTTATCACTCGCAGTCTGACTCCCAAGGA32020.13945464610008246No Hit
GTGAAATTCCGTCGGAATCCGGGAGGACCATCTCCCAAGGCTAAATACTC31810.13854004660973213No Hit
ATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCACGTGT31750.13827873246963204No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAA31380.13666729527234814No Hit
CGGCATTCTCACTTCTAAGCGCTCCACCAGTCCTTCCGGTCTGGCTTCAC31090.13540427692853102No Hit
CGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACA30950.13479454393496415No Hit
GTGGGATTGGCTTAACCTCGCGGTTTCGCTGCCCTTTGTTCTGTCCATTG30940.1347509915782808No Hit
AAAGGAACGAGGTAGATGAAGAGGTCTGGAAAGGCCCGCCATAGGAGGTA30860.13440257272481398No Hit
CTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACGGATAG30730.13383639208793047No Hit
CTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCTGCCTTCTCA30500.13283468788421346No Hit
CTCAAATTTCCTGCGCCCGCGACGGATAGGGACCGAACTGTCTCACGACG30170.13139746011366296No Hit
CCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGA29600.12891497578271205No Hit
CCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCC29500.1284794522158786No Hit
GTGGGTTCGGGCCTCCATTCAGTGTTACCTGAACTTCACCCTGGACATGG29200.12717288151537812No Hit
CTGGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTT29140.12691156737527803No Hit
ATTCGGTGGAGGGGATTCTCACCCCTCTTTCGCTACTCATACCGGCATTC29080.12665025323517792No Hit
GGAAAGGTGAAAAGCACCCCGGAAGGGGAGTGAAAGAGATCCTGAAACCG28980.12621472966834443No Hit
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCA28790.1253872348913608No Hit
CTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAAACCCACCACT27870.12138041807649275No Hit
GGAGTATTTAGCCTTGGGAGATGGTCCTCCCGGATTCCGACGGAATTTCA27790.12103199922302596No Hit
CTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCACCGGATCACTA27710.12068358036955917No Hit
GACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAG27490.11972542852252549No Hit
CTTAGCTGGCGGTCTGGGCTGTTTCCCTTTCGACTACGGATCTTATCACT27430.11946411438242541No Hit
ATTGAATGTTGGTACAGCTTGTACAGGATAGGTAGGAGCCTTGGAAACCG27320.11898503845890858No Hit
ATCTCTTTCACTCCCCTTCCGGGGTGCTTTTCACCTTTCCCTCACGGTAC27320.11898503845890858No Hit
GTCAAACTCCGAATGCCAATGACTTATCCTTGGGAGTCAGACTGCGAGTG27290.11885438138885852No Hit
GTTCCGACCCGCACGAAAGGCGCAACGATCTGGGCACTGTCTCAACGAGA27150.11824464839529164No Hit
CTCCTCTTAACCTTCCAGCACCGGGCAGGCGTCAGCCCCTATACTTCGCC26740.11645900177127434No Hit
GGAAAGGCCCGCCATAGGAGGTAACAGCCCTGTAGTCAAAACTTCGTTCT26580.11576216406434077No Hit
GGCGCACGGTGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAA26400.11497822164404049No Hit
CTACAACCCCAAGAGGCAAGCCTCTTGGTTTGGGCTAATCCCGTTTCGCT26040.11341033680343994No Hit
CTTGGTTTGGGCTAATCCCGTTTCGCTCGCCGCTACTCAGGGAATCGCAT25810.11240863259972293No Hit
CGATATGCTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAA25780.11227797552967288No Hit
CGACGGGGAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGGGCTG25700.11192955667620609No Hit
TGGATGCCTTGGCACTAGGAGCCGATGAAGGACGGGACGAACACCGATAT25690.11188600431952272No Hit
CTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCAGGGTTAGTCGGGACCTAA25450.11084074775912238No Hit
CGATGAAGGACGGGACGAACACCGATATGCTTCGGGGAGCTGTAAGCAAG25300.11018746240887213No Hit
GTCGGTCCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTG25060.10914220584847176No Hit
GTCCTCTCGTACTAAGGACAGCTCCTCTCAAATTTCCTGCGCCCGCGACG25060.10914220584847176No Hit
GTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCGTCA24740.10774853043460461No Hit
CTTCGGGGAGCTGTAAGCAAGCTTTGATCCGGAGATTTCCGAATGGGGAA24720.10766142572123791No Hit
GTTTCAGTTCCCCGGGTCTGCCTTCTCATATCCTATGAATTCAGATATGG24650.10735655922445447No Hit
GTCGCCTGGGCCTATTCACTGCGGCTCTCTCGGGCTTGCACCCTAACAGA24510.1067468262308876No Hit
ATTTTCGGCGCAGAGTCACTCGACCAGTGAGCTATTACGCACTCTTTAAA24440.10644195973410414No Hit
GTCCCCCCATTGCTCAAATGGTGAGGAGGTGGTACAGGAATATCAACCTG24110.10500473196355364No Hit
ATTTGCTTTCTCTTCCTCCGGGTACTTAGATGTTTCAGTTCCCCGGGTCT24030.10465631311008686No Hit
GTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTGCCAGCATTCAG24030.10465631311008686No Hit
CTAAGGGCTGTGAAGCCAGACCGGAAGGACTGGTGGAGCGCTTAGAAGTG23930.10422078954325338No Hit
GTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCGGGCT23780.10356750419300315No Hit
ATCCCCTCCACCGAATGCCTAAGGTTTCCTGAGGAAGGCTCGTCCGCTCA23760.10348039947963644No Hit
CAGGAATATCAACCTGTTGTCCATCGCCTACGCCTTTCGGCCTCGGCTTA23250.10125922928878567No Hit
CCCGGGGTAGCTTTTATCCGTTGAGCGATGGCCCTTCCATGCGGAACCAC23160.10086725807863552No Hit
GTGCCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTG23120.10069304865190214No Hit
GCCTTCTCATATCCTATGAATTCAGATATGGATACCACTCCATTACGAGT23100.10060594393853543No Hit
CTGTGTTTTTGCTAAACAGTCGCCTGGGCCTATTCACTGCGGCTCTCTCG22990.1001268680150186No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCACGT150.002222945770.017791
GCTAGCG150.00222525169.999510
CCCATGG150.00222525169.999510
CCTCGGT550.063.6525421
GACACTA800.061.2495612
TTGACAC800.061.2495610
ACACTAG800.061.2495613
GGCGCAC4200.059.181711
GCGCACG4250.057.6604582
TCACTAC509.840733E-1055.99968
GGACCTC252.3916263E-455.999611
AGATATA407.4880336E-752.49962269
CGATTCG200.006953113252.4996228
TACTCCG200.006953113252.49962214
ATAGTGC200.006953113252.4996225
TGCGTTG200.006953113252.49962223
TGATCCT4400.051.7041744
TTGGGGA552.2882887E-950.90872613
CCCTATG1000.048.9996514
ACTAGGC506.205482E-848.999657
Produced by FastQC (version 0.11.2)