FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_pr8_positive_control_nw_cb7-9.35200000088748.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_pr8_positive_control_nw_cb7-9.35200000088748.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102194
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4800.46969489402508957No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4570.44718868035305404No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT3160.309215805233184No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT3080.3013875569994324No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2410.2358259780417637No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA1710.16732880599643815No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG1560.1526508405581541No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1530.14971524747049728No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1490.14580112335362155No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1480.1448225923244026No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG1450.1418869992367458No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1430.13992993717830793No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1410.13797287511987005No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1410.13797287511987005No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT1330.13014462688611855No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG1310.12818756482768068No Hit
GCATCCACAGCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTT1280.12525197174002387No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1240.12133784762314813No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1170.11448813041861558No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1170.11448813041861558No Hit
CTCCTATTGTGACTGGGTGTATATTCTGGTAAGGGAGACTGCTGTTTATA1100.10763841321408303No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG1030.10078869600955045No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGTA100.007081611145.01
GAGCGAA700.0145.01
TTAGTAG100.007081611145.02
CATAACT100.007081611145.03
AAAGGTG100.007081611145.0145
GCAAAAG2850.0104.298253
AGCGAAA1050.096.666672
AGGGTGT301.5925601E-596.6666646
AGCAAAA3250.093.692312
GCAGGGT1600.090.6259
AAGCAGG4700.083.297877
AAGGGTG353.4224933E-582.857145
GCAGGGG1000.079.759
AGCAGGG2950.078.644078
CAAAAGC3950.073.4177254
GTATTAG702.7794158E-972.51
GAGCAAA4700.067.872341
GGCGTCT651.3855242E-766.923077
AGGCGTC651.3855242E-766.923076
TAGGCGT651.3855242E-766.923075
Produced by FastQC (version 0.11.2)