FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_pr8_e3_l_cb7-9.352000000889f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_pr8_e3_l_cb7-9.352000000889f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176152
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA5720.3247195603796721No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG4780.2713565557018938No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC4440.2520550433716336No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA4300.24410736182387938No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC4040.22934738180662154No Hit
CTCCAACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCT3690.209478177937236No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT3580.2032335710068577No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA3490.19812434715473No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG3470.1969889640764794No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG3400.1930151233026023No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG3250.1844997502157228No Hit
ATATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT3230.1833643671374722No Hit
ACTCTATGCTGACAAAATGACCGTCGTCAGCATCCACAGCATTCTGCTGT3140.17825514328534448No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCTCCATTCCC3120.17711976020709386No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA2910.16519823788546256No Hit
CCTGTAACCGTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGA2610.14816749171170351No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC2520.14305826785957582No Hit
GTCGTACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA2520.14305826785957582No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG2470.14021981016394933No Hit
CAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG2460.13965211862482402No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA2400.13624596939007222No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT2390.1356782778509469No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA2350.1334075116944457No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC2350.1334075116944457No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA2330.1322721286161951No Hit
GTACTGGAGCTAGGATGAGTCCCAACGGCTCTCATTGCCTGCACCATTCG2330.1322721286161951No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT2290.1300013624596939No Hit
GATATTGAGAGGGTCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT2260.128298287842318No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC2230.1265952132249421No Hit
CCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGT2180.12375675552931559No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTTGCTTT2160.122621372451065No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATCGGGACAA2150.12205368091193969No Hit
TTTTTAAACTATTCAACATTAATTGATGGCCATCCGAATTCTTTTGGTCG2150.12205368091193969No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2110.11978291475543848No Hit
CTTTTAAGGCCGTGTTTGAAGGGTCGATAGACGCATTTGAAAAAAAGACG2100.11921522321631317No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG2080.11807984013806258No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT2060.11694445705981198No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA2060.11694445705981198No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT2030.11524138244243608No Hit
ATTCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA1970.11183523320768428No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA1900.10786139243380717No Hit
ATACAATAGAATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT1810.10275216858167946No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG1780.10104909396430356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGT203.8607142E-4108.749995
GTAACCG203.8607142E-4108.749994
GCAGGGG406.096434E-790.624999
CGAAAGC950.083.947373
TAGACAG353.4294048E-582.857145
AGCGAAA1000.079.751
CAGTACG406.6479726E-572.499999
GCAGGGT1800.068.472229
CAGAACC451.19113975E-464.444449
ACAGTAC451.19113975E-464.444448
TTAGACA451.19113975E-464.444444
TAGAAAC1350.064.444442
CAAAAGC5500.063.2727284
ACTATTC350.003561628762.1428578
TAAACTA350.003561628762.1428575
AGGGTAT350.003561628762.1428576
TACATCA1400.062.1428574
AGGCATC350.003561628762.1428576
TCATGGT1500.058.08
AGCAGGG3050.057.0491838
Produced by FastQC (version 0.11.2)