FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_negative_rt-pcr_control_l_cb7-9.35200000088ad8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_negative_rt-pcr_control_l_cb7-9.35200000088ad8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGAAACTGATTGCCCCCAGGGAGACTACCAGTACCAATGAACTGGCG21.550387596899225No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA21.550387596899225No Hit
GTAGTAGAGATACTATCGCAAGTATTTATTATTGAGGTTTAATTGGCTAT10.7751937984496124No Hit
CCATTAGACGATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGG10.7751937984496124No Hit
ATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTTAT10.7751937984496124No Hit
CATTAAACGCAATGCCGATAAGGAGAGTTATCGGCATTGGACGCGAGCCA10.7751937984496124No Hit
GGATCAAGTCCCGCACCGGACTCCCCGCTCAGGGGGGACCCAGCCTGTCT10.7751937984496124No Hit
CCACGATGGAAGCTGCCGCCTCCACGCCCGGCGCGATCATCGCGCGGCTC10.7751937984496124No Hit
AGCCTGAGAGGATTATGAAAACATGGCAACGGAAGTGAGGCCAGGGGACA10.7751937984496124No Hit
GATTAGGAGTACACTTTCTGTGAGAAGCTCTGAGTAACTGCTGAGTCACT10.7751937984496124No Hit
CTGGAATGATGACCTGAGCTGAAGGCAGAGGATTAACCAATGAGCCAACC10.7751937984496124No Hit
CCAGTGCAGCCCCAAGACGAGCCTGCAGTATTTTCCTTACATGATCTGGT10.7751937984496124No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA10.7751937984496124No Hit
ATTTTGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAG10.7751937984496124No Hit
CTGTATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGAC10.7751937984496124No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTCATTACCAA10.7751937984496124No Hit
ATGTATCATAGTCGTTTTTTTATCTTTACCCTTAGTGGAATTCATTGAGC10.7751937984496124No Hit
GCTGTCCGTTCGTACTGAAGCCCGCGAGCCGCACGCCGATCGGCGCGTTG10.7751937984496124No Hit
GGATTGAGTCTCACATTGGGCTCCTTGTCCAGCAGGGAGCCTGCTTCTCT10.7751937984496124No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC10.7751937984496124No Hit
GCTTTCGCTCCCCCCGGCCTCCTTCCTCATCCGCAGTCTTCTCTTGAGTT10.7751937984496124No Hit
ACAAAATGAGCAATGTCTATTTTCTATTTAACATCTCTCCCTAACACAAC10.7751937984496124No Hit
TCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCT10.7751937984496124No Hit
GAGCAAAAGCAGGGGAGAAGGGTAGAGGTAGAGGGAGAAGCAGGCTCCCT10.7751937984496124No Hit
CGATATCCGCGAACGGCTCGTCCCGGTCGATCAGCGCATCACTGCCCAGG10.7751937984496124No Hit
GCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGGCG10.7751937984496124No Hit
CGCCGGGCCGCATCATGGATCACCTCGACCGCGGCAACCTGAAGCTCGAC10.7751937984496124No Hit
ATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCAT10.7751937984496124No Hit
CTACTTTTCCTAAAAATTCCAGCGCAAAATGCCATAAGCACCACATTCCC10.7751937984496124No Hit
GCAGAAAGTCTGCTTCTCCCTCTTTTTTTTTTTTTTAAGATTTTATTTAT10.7751937984496124No Hit
GTTCCCATCCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGT10.7751937984496124No Hit
CCCTAATACTGTAGATACACTATACAGAAATGAAACAGAAACTGGAAACA10.7751937984496124No Hit
GCCTGAAGCTCAACTATCCGGAGGCGGTCGCCTTCATCACCGCCGCGCTG10.7751937984496124No Hit
CTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTA10.7751937984496124No Hit
GGCTTCCCACTGAGCAGGGAGTCAAATGTGGGGCTCCATCCAAGGACCCT10.7751937984496124No Hit
GGTCAAATGTATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG10.7751937984496124No Hit
GCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCT10.7751937984496124No Hit
TTAAAAGCGTGTTTTTTATCTTTATATAATCTCAAACCAGACCCCTCTTA10.7751937984496124No Hit
AGATCGTCATGTGGGAGACACTGACTACCCACTCCTAGCAAAGGGCTCAG10.7751937984496124No Hit
TCCTCAACCCTTATCCTATCACATTCACCTTCTATATCTTTTGTTTTGCT10.7751937984496124No Hit
TTACTCCTGTCTTCCGGGCTCACGCCCGGCCCCCCACCCCCTCCGCCAGC10.7751937984496124No Hit
TCAATACTGGATCAGCGGGTCCACTCCTCCCCGCCTGACAGGGGATAGTG10.7751937984496124No Hit
AACAAGGACCCTGCGTCGTGGCTTGATACCAGGACTTTGGAATCATGACC10.7751937984496124No Hit
CATTCCCAACAGCAGAATTTTAAGTGTTCTAGTTTCTCTACCTCCGCATC10.7751937984496124No Hit
GTAGAAACAAGGCGAAGGATGTCATCGGTTTCCCTTGCCCTTTCACGTAA10.7751937984496124No Hit
ACTCCTTCTGCGCCCGGCGCCCAATATGTGACTTGATCATAGGACTCTGG10.7751937984496124No Hit
GAGCAAAAGCAGGAGGATAGCACTTGATCATACATGAGTCAAGGAGGAAT10.7751937984496124No Hit
TTTCAAAACATGGGGTCCAAAGGAGAAGGGAGAAAAAAATAACAGAAAAT10.7751937984496124No Hit
GGAGTGGGTAGTCAGTGTCTCCCACATGACGATCTTGCTTCATTCAGATA10.7751937984496124No Hit
TTGCCTGCACTGCATTTTACTTCCGCAGCCTAATCCCTGTCAGTCACCAC10.7751937984496124No Hit
ATTGCATGTCGACCAATGACACGAGATGTATTTCCACTAGTTTTAGCTAG10.7751937984496124No Hit
CTATTGGAAAATGAAAGAACTTTGCACTACCACGATTCAAATGTGAAGAA10.7751937984496124No Hit
GAACCTCCTCAAAAAAAAGACTCAGGAGGACCAAGCGGTGTAACCAGACT10.7751937984496124No Hit
CCATGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATATAGGAGACC10.7751937984496124No Hit
GAAGCAAGATCGTCATGTGGGAGACACTGACTACCCACTCCTAGCAAAGG10.7751937984496124No Hit
AACTAGATCCGGTCACATTTGTCCTTTTTGGAGTCACTCTGAGAACTTCT10.7751937984496124No Hit
GCGATCATCATCAAGATAACTAATCAAGAAATCAAAGCTAAAAAGAATGT10.7751937984496124No Hit
CGGCAAGTCGCCGATCTACTTAGCACAAATATATCCATTGCCACTACTAC10.7751937984496124No Hit
CTTCGAGTCTGGACCGTTCAAGCAAGAAGTGTTCTCTGAGATCATGAAGA10.7751937984496124No Hit
CCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACA10.7751937984496124No Hit
CTCCAGACTGGGTGACAGAGCGAGACTCTGTCTCCAAATAAAAAAAAAAA10.7751937984496124No Hit
GTGTATTTGTAGGATAGCCATTCTTTGGCACTGTTCCGCCTTTGGTCTTT10.7751937984496124No Hit
GAGTGGCTGTTTCGATATCGAGGCCAAGGGCGTTGCCTCTTCCTTTTAAG10.7751937984496124No Hit
GTTTGCTGCAATATGCACACATTTGGACGTTTGTTTCATGTATTCGGATT10.7751937984496124No Hit
GACCTACTCAGGCTAAATCCATCATCAACACAGTATTTTCCCATTAGAAC10.7751937984496124No Hit
GATAAGTCTAAATGAATAATGGCCAAGACAGTAGTTAAAAGCCTACAGGG10.7751937984496124No Hit
GGGAGAGGCAGAGAGAGAGGGAGAAGCAGGCTCACCGCTGAGCAGGGAGC10.7751937984496124No Hit
GCTCAGTTGGTTAAGTGTTTGCCTTTGGCTCAGGTCATGATCCCAGGTCC10.7751937984496124No Hit
CTTACACAGTTTGAGCATTCACCGGCCACTAGAAGCCCATCTGTCGCATT10.7751937984496124No Hit
AAGTGATACAAATGAACAGAAAATTGAAAAATTCAAATGCTTGACAATCT10.7751937984496124No Hit
TTCATTTTGATGGTGATAACCGTACCATCCATCTACGATCCCTGTCCACC10.7751937984496124No Hit
TACAAGGTGTCTTTCGTGCCTGTCGCCCTTTTTTTTTTTCAGTACTCTGG10.7751937984496124No Hit
AGATAAAAATTACACTGGCCATTCACCAGAAAAAAAAATAAACTCAAGCT10.7751937984496124No Hit
GGGATCACGCATGACCTGAGCCTAAGGCAGATGCTTAACTGACTGAGCCG10.7751937984496124No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA10.7751937984496124No Hit
GGGTTCTCAGCAATCTGCCTTTTTCCTCCATTGCCTCCAACAGATCATCC10.7751937984496124No Hit
GGCCTTTTCTCTGCACTGTCTGGCAAACTCCCGCCTTGAGGCCAACTGTC10.7751937984496124No Hit
GTACAGAACTACTCAACAATTTAGTTGTAGGGGCACCTGGGTGGCTCAGT10.7751937984496124No Hit
TTGGAATAGGAACCGGATACCCCATGTAAAGCGTAAATAGAGTTCGAAAA10.7751937984496124No Hit
TCCCTTGGGTTAAGTGCAACACTTCAATATAAACACTGCCTGTTCCGCCG10.7751937984496124No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT10.7751937984496124No Hit
GGGAGTGGGAGATGGAGAAGTAGGCTTCCCGCTGAGCAGGGAGCCCGATG10.7751937984496124No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT10.7751937984496124No Hit
GGAATGATTGTCATTTAGCAAGGCGCATTGCGCCAATAAACATGATCTGT10.7751937984496124No Hit
ACTGTGTCCTCTTGGTAGCTAAAAGAATCTGCTAACAGTCAAATTATAGT10.7751937984496124No Hit
GTTCGAAACAAACCCAAACGTGACACATAGGGTATTAGTAGACAAGCACT10.7751937984496124No Hit
ATACGAAACCTCTACTCTGCATCCCAGGTGTTGCGATAGCCCATCTTTTT10.7751937984496124No Hit
CAAGTAACAAAGTATACTTCTGTTCTTGTGGAAGGACGTCAAAAACATTA10.7751937984496124No Hit
GGCCACATCTTCCCCGAGGGCATCCCCGCAGGGAGGCCGTGTGCTGCTGA10.7751937984496124No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT10.7751937984496124No Hit
ACAACTTCCATAATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATG10.7751937984496124No Hit
TAAGGGAACCATTCATATCATGCTCCCCCTTGGAATGCAGAACCTTCTTC10.7751937984496124No Hit
CTATTTACTCATTTGAGCCCATCAAGAAATACATTGCACCCAGTTAAACT10.7751937984496124No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA10.7751937984496124No Hit
ATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTCC10.7751937984496124No Hit
GAGCAGCGAGCCTGATGCGGGGCTCGATCCCAGGGCCCTCAGATCATGAC10.7751937984496124No Hit
TGCCCCTATTTTTACGGTTCATATTTCTTATAACCTGTGTGCTCATATGT10.7751937984496124No Hit
ATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACT10.7751937984496124No Hit
CCAGTCATCTGTCTGCGGACATTTGAATTATTCTCGCCCCGCAGCATTAA10.7751937984496124No Hit
GTGGCAATTGAGGAACACAATTCAAGTGATATATTATTCCCTTTGACTAC10.7751937984496124No Hit
AAGCACCACATTCCCTTATACTGGAGATCCTCCATACAGCCATGGAACAG10.7751937984496124No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG10.7751937984496124No Hit
ACGTGTTTCCAGGGAAGGCGTCTATGATGGATGCGATTTAAAAAGGGAGG10.7751937984496124No Hit
ATATAAAAGAACTGAATATAACTTCTAGGACTAAAAAAATACAATATCTG10.7751937984496124No Hit
GAGGGAACTGCAGGCTCCCCACTGAGTAGAGAGCCCAATATGGGGCTCCA10.7751937984496124No Hit
TTGCCACACCAACTTCAGTGCATGTGCGAGGAAGGAGTTGAAACAAGAGG10.7751937984496124No Hit
GTCGCACGACGCGGCGCCGGCCGAAGCGGCTGCCGAAGCGCCGGCCGCTG10.7751937984496124No Hit
ACATAATGGACTCCAACACCCTGTCAAGCTTTCAGGTAGACTGTGTCCTT10.7751937984496124No Hit
TTAAGACCAAGGTTGCGGATAAGCCGATGAGACCGTATTAAAGATAGGGC10.7751937984496124No Hit
TCTATATACAGTAAAGACAACCATCTAATAATCTGTTCCACGGTGGATTT10.7751937984496124No Hit
GTACCAGAACGTCGAAGTGAACGGCAAGTATCAGTTCACGCCGGCCTTGT10.7751937984496124No Hit
CCCTAGAGTAATTCTCAATCCTTGGTTGCAAATTAGAATCACCCAGGGAG10.7751937984496124No Hit
GCTCAGGAGTCTGCGATCTCTCGGTCGCAAAAGAAACTAACCCGGTCGTG10.7751937984496124No Hit
AGTTTATTTATTTGACAGAGAGAGAAGGAAAGAGAGCAAACAGGGGGAGC10.7751937984496124No Hit
CTTAAGCGAGCAGAAACATACTCCATGGTACATAAGATTTATCATCTGTT10.7751937984496124No Hit
ATCAAGGACAAGCTCGGCGCCCGCTCGGTCGAGGTCGCCGAAGCCGATGC10.7751937984496124No Hit
GAACTACAAAGACCCATGTATTTTACCTTTTAACATGATCAGTTCACTTA10.7751937984496124No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT10.7751937984496124No Hit
GAGGTGAAGGCAGACAATCGACTGAGCCACCTAGTTGCCCTGGTTAATCT10.7751937984496124No Hit
GTACTTTTTTCTTATGTCCATATGGTTCACAGTGGTCTTATTGAGTCTCT10.7751937984496124No Hit
CTGTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGC10.7751937984496124No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG10.7751937984496124No Hit
GTTCAAAGGCGCGCAAAGTAGAGCAGTACTGTGTTCCCATTCGGTACCCC10.7751937984496124No Hit
GAGAAAAAAAAAAAAAAAAAGACAAGGGGCGAAGGAGGGGTCACAAAACA10.7751937984496124No Hit
AAGAAGGGAGGGGAGAAGGAAGGAGAAGGGAAAGAAAAAAGGAGAGAGGA10.7751937984496124No Hit
CTCGCGCTGGTGCGCAACTACCTGCCGTCGCATGCGATCGCGCAGCAAGG10.7751937984496124No Hit
TGACTCAGAAAAGCAGTGAGCTACGAAAGCTATCTGAATGAAGCAAGATC10.7751937984496124No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers