FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_negative_extraction_control_nw_cb7-9.35200000088761.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_negative_extraction_control_nw_cb7-9.35200000088761.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172825
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGGTGTCCACACGGGCATGTTGGGTGTGGGGACAGCAGCTCTGC6140.35527267467090984No Hit
GTCCATACAGCGAGGACCATTTGCCTTAGGAAGGAACGGATCCCACAGGG4940.28583827571242587No Hit
GTCCAGGTGTGGGCACAAGTCGGGGGTCAGCTTGCCTTGACTTCGGGCAT4140.23954867640676986No Hit
GATTAAGAAGGCCCGGGTCACAGGGCTCCAGGCACCCCGTGCGGTCAGTC3920.22681903659771446No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT3880.22450455663243166No Hit
TCCTATGTCTCTCTTGCCCCGCGTGGGGAAGTCCTGTGTCAGGCCTTTGT3360.19441631708375526No Hit
TTCAAGGACAGATTAAGAAGGCCCGGGTCACAGGGCTCCAGGCACCCCGT3150.18226529726602053No Hit
CTTCCGTAGGAGCACGTACCTCAGTTGCTGGAACATTTTGCTCATGACTC3120.18052943729205845No Hit
GAGTATGATGTTGGCAGCTTTGTCCCTCGAACTGGCCTGACTCTGCTGAG2970.17185013742224794No Hit
GAATTAAGTTCCTCCCTTGCATGAGAATGGATCCCAACCGTGACAGCTGC2930.16953565745696514No Hit
AGATAATACAGGCCAAGAGGTTAGTGCAGAACCCACACTGTGGTTAAGGG2850.16490669752639955No Hit
TCCCTGAACTGTCTCATGGTGATGGGTGCCACGGAGTAGTTCTCTGCTCA2840.16432807753507883No Hit
AGCGAAAGCAGGGCATCTCAAAATCACCTGCAGGAACACGTCTGAAAATG2770.16027773759583394No Hit
TCCCTGTGGGATCCGTTCCTTCCTAAGGCAAATGGTCCTCGCTGTATGGA2650.15333429769998555No Hit
GGTCTGGGCAGCTCCTGGGTCCTGCCGGGGATGGGAGGGCCACTGGGTAA2630.15217705771734413No Hit
GCCCCACACAGCCCTCGGCCTCAGCCTGAATCGACTGCTATGTGAGCTGC2620.15159843772602344No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC2590.14986257775206133No Hit
TTCTTAATCTGTCCTTGAACGGTGAGCAGAGAACTACTCCGTGGCACCCA2580.14928395776074063No Hit
GTCCTGCAGCTGTCACGGTTGGGATCCATTCTCATGCAAGGGAGGAACTT2550.14754809778677855No Hit
TTCACCAGCCGAGTGGGCCTCCAAGCCGTGCTGTCTCAGCAGAGTCAGGC2500.14465499783017502No Hit
CACCTGGACAACGATGTTGAAACCCTGAACCGACTGAACGCCCCAAGTCC2500.14465499783017502No Hit
GTATGGACAGACCACATGTCCCTCACACGTTCACCAGCCGAGTGGGCCTC2490.14407637783885432No Hit
TTCCAGAGGCAGGTGTGTCCTCAGGATGCAGTAAACCCCATGCGCTGTTG2480.14349775784753363No Hit
TCCCTCACACGTTCACCAGCCGAGTGGGCCTCCAAGCCGTGCTGTCTCAG2450.14176189787357155No Hit
CTGTATGGACAGACCACATGTCCCTCACACGTTCACCAGCCGAGTGGGCC2360.1365543179516852No Hit
GCTCAGAGCCACCCCGATGCCAGGTACAGGAACCACGGGACCCCTTCTCT2270.13134673802979893No Hit
GTCCACACGGGCATGTTGGGTGTGGGGACAGCAGCTCTGCCAACACACAC2240.12961087805583682No Hit
GTTCAGGGTTTCAACATCGTTGTCCAGGTGTGGGCACAAGTCGGGGGTCA2230.12903225806451613No Hit
GTCTCATGGTGATGGGTGCCACGGAGTAGTTCTCTGCTCACCGTTCAAGG2160.12498191812527124No Hit
AGCGAAAGCAGGGTGTCCACACGGGCATGTTGGGTGTGGGGACAGCAGCT2130.12324605815130912No Hit
GCCCAGACCCCATGCCCGAAGTCAAGGCAAGCTGACCCCCGACTTGTGCC2110.12208881816866772No Hit
GTATGATGTTGGCAGCTTTGTCCCTCGAACTGGCCTGACTCTGCTGAGAC2090.12093157818602633No Hit
GTGTGGGGCAGCTGGCTTCTTGCTGTGGGTCCCCACAAGGCTCTCTGGGT2030.11745985823810212No Hit
GACTTGGGGCGTTCAGTCGGTTCAGGGTTTCAACATCGTTGTCCAGGTGT2010.11630261825546072No Hit
GGGTAAGGTGATGCGGCAGCAACCATGTGACTATCAGGACACTTCCGTAG2010.11630261825546072No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG2000.11572399826414004No Hit
TCCCCACGCGGGGCAAGAGAGACATAGGAAGTGCCGGGACCCCCAGAGCA2000.11572399826414004No Hit
AAGCAGGACCAGGCCCTGGGTGAAGTGGCCTCCTCCTTCCAGAGGCAGGT1960.11340951829885723No Hit
TATCAGGACACTTCCGTAGGAGCACGTACCTCAGTTGCTGGAACATTTTG1920.11109503833357443No Hit
CATCATACTCAGAGGGAGACAACGCGACTGACCGCACGGGGTGCCTGGAG1910.11051641834225372No Hit
GTACAGGAACCACGGGACCCCTTCTCTGTGTTCCACCTTGAATTAAGTTC1910.11051641834225372No Hit
CCCCAAGTCCATCTTTTCAGACACTGAGGGAGGCCCCTGAGAGCAGCTCA1890.10935917835961231No Hit
AATGAACAGTGACCGGTCACCAACAGTCAGTCTGTCCTGCAGCTGTCACG1880.10878055836829163No Hit
GTTGGTGACCGGTCACTGTTCATTTTTGCATTTTCTGAGTCATGAGCAAA1880.10878055836829163No Hit
GTCCTTGAACGGTGAGCAGAGAACTACTCCGTGGCACCCATCACCATGAG1850.10704469839432952No Hit
GTCTCAGCAGAGTCAGGCCAGTTCGAGGGACAAAGCTGCCAACATCATAC1850.10704469839432952No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT1840.10646607840300883No Hit
GACATAGGAAGTGCCGGGACCCCCAGAGCAGTGTTTCCCTGAACTGTCTC1830.10588745841168812No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC1800.10415159843772603No Hit
TCCTAAGGCAAATGGTCCTCGCTGTATGGACAGACCACATGTCCCTCACA1770.10241573846376392No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG1740.10067987848980182No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGG100.007085824145.0145
GTCCTAA151.228259E-4145.01
GAGCGAA400.0145.01
CAGTCTT100.007085824145.09
AGCGAAA1750.0111.8571551
CGAAAGC2050.095.48783
GCGAAAG2050.095.48782
GTACAGC502.2575477E-887.01
ATGAATC259.373517E-487.08
AGGCTTA259.373517E-487.06
CAAGGTA353.4292192E-582.857148
TTAAGGC353.4292192E-582.857143
CGGAGGG554.3728505E-879.090917
CCTAAGA300.001933024472.53
GCAGGTA406.647614E-572.52
ACAAGGT406.647614E-572.57
TTAGACA350.00356151462.1428574
GGCTTAT350.00356151462.1428577
CTCTATG702.3231405E-762.1428572
CTGATCC350.00356151462.1428579
Produced by FastQC (version 0.11.2)