FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_extraction_control_l_cb7-9.3520000008897c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_extraction_control_l_cb7-9.3520000008897c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15732
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAGAGAGGGAACACGAGCAGGGTGAGTGGGAGAGGGAGATGCAGGC1350.8581235697940504No Hit
ATATAATGTGAAGTGAGAAGGCAGACTTCCCAACAGTGTGCAAAACAACC510.32418001525553014No Hit
TCCCAACAGTGTGCAAAACAACCTCATTTTCATTATAAGACATACCACAT500.3178235443681668No Hit
GGGTAAGGATGGTTTCTGCCTAGAAAACCAGGACTTCAAATTTGCAGGGA500.3178235443681668No Hit
GTATAAAGGAGAGTTTAGGGGAGGGCCTGATTTGAAGAGAGGAGGGAGGA490.3114670734808035No Hit
GTTGAAGGGTTTGCATTCTCTCCCTGGGTTCAGTCTCTCTCTCAGCACCC480.30511060259344014No Hit
ATTTAGACATTGAACAGATCATCTTTGCTAACCGTTGGTATTTGGTGGGT470.2987541317060768No Hit
CCATTGATGTATTGGGGAAAAAAAATCACTGTGGGAAAGAAAGCCTGGGT460.29239766081871343No Hit
CCTCTAAGCAGACTCCCTCCTGAGCAGAGAGCCCAATGCAGCCAAAGGCA430.27332824815662343No Hit
GTTTAGGGGAGGGCCTGATTTGAAGAGAGGAGGGAGGAGATGGCAGGATA410.2606153063818968No Hit
GTATTACTCATTTCCTGTGAATGACTGTTGTAGATCTTCATCCAAAGATG390.24790236460717008No Hit
TTATATAACAGCCCACAAATATTTTTCAAAACATGGCAAAGGGATCTGAC380.24154589371980675No Hit
TCTGTAGACTGTAGTTTTGAGAACAGAGTTGCTAAGGGTCTCAGAGGGGT370.23518942283244343No Hit
ATGTATAAGTAGCTTGTGTAGCTGTTTTTTTCTTTCCTTTCCTTTTTAGG370.23518942283244343No Hit
CCCTTCAACAGTGTAAAGCAAAGGGGCTCAAGTTTATATAACAGCCCACA350.22247648105771675No Hit
ATCTGGAGCATGGTATAAAGGAGAGTTTAGGGGAGGGCCTGATTTGAAGA330.2097635392829901No Hit
TTATAAGACATACCACATAACCAAACTATTGATCAAGGTTGTACCTGCAG330.2097635392829901No Hit
CCCCAAGACTGGCAAATATTTTTTGTTTTGTTTGGCAAACATTTTGATAA330.2097635392829901No Hit
TCCTTACCCAGGGTAACCACAGTTACTTCTTTATTAGTTGACTTTCTCTG330.2097635392829901No Hit
TCTGAATGCTTCCCCATCTTTAAAATGGGAATAACGAGGGGCGCCTGGAT310.1970505975082634No Hit
AGGTACACATACTCAACAAGGTTTTTAAGAATCCACAATGAGGGTTGCTG310.1970505975082634No Hit
CCTTTATACCATGCTCCAGATAGGGTGCTGAGAGAGAGACTGAACCCAGG310.1970505975082634No Hit
CTTCAAGACTAATGAGGGACAGAAAATGTAGACAGGAAAGGGGGCAGGAC300.19069412662090007No Hit
GTGTAAAGCAAAGGGGCTCAAGTTTATATAACAGCCCACAAATATTTTTC300.19069412662090007No Hit
CTCTAATGAGAGACCACAGGACACCTTAACCACAATAATAGTTACTGACG300.19069412662090007No Hit
TCTCTGTACATATTATTTTTCTCCTTTATCTACTTTTTAAGGCACAATTT300.19069412662090007No Hit
AATATACCCTTTGACACAGAAATTCTGCTTCTCAGAGCTTACCCTCAGGA290.18433765573353675No Hit
ATCTAATAGTCAATTTATCTGATAGTTATTAAGATACGAAATATCTGAAG290.18433765573353675No Hit
GCTTTACACTGTTGAAGGGTTTGCATTCTCTCCCTGGGTTCAGTCTCTCT280.17798118484617342No Hit
AGCGAAAGCAGGGGGAAGTGGCAGGGAGAGGGAGAACGAGGCTCGTGCTG280.17798118484617342No Hit
GTCTAGGAAGAAGTAATTCAGAGAGGGAAGGTGGAAAATGAGAAGTGGCC280.17798118484617342No Hit
GTATACTATGCAGCACTTAAATGACACAAGGTACACATACTCAACAAGGT270.17162471395881007No Hit
ATCTATGATTACTAATTTACTCTCCCACCAGCAATGGGAAAGGATGCCTA270.17162471395881007No Hit
AGTTTATATAACAGCCCACAAATATTTTTCAAAACATGGCAAAGGGATCT270.17162471395881007No Hit
GGGTAGAAGAAATGAAATCAAATTCACCTAGCAACAGAGAAAGTCAACTA270.17162471395881007No Hit
ATATAAACTTGAGCCCCTTTGCTTTACACTGTTGAAGGGTTTGCATTCTC270.17162471395881007No Hit
CTTCTACCCTTTGTTACATTCTTAAGAATGGAAATCCGTTGTTATTGTAA270.17162471395881007No Hit
TCCCAGGACCCTGAAATGACCTGAGCTGAAGGCAGACGCTTAACGATTGA270.17162471395881007No Hit
GATCAAGGTTGTACCTGCAGGATGAGATGCAGGGGATTTATTTTACAATA260.16526824307144672No Hit
GGGTTGTATTGACCAGAAGTGTGATTTCCTAGCTTTAGGAATTTGTACAG260.16526824307144672No Hit
GGTACACATACTCAACAAGGTTTTTAAGAATCCACAATGAGGGTTGCTGG260.16526824307144672No Hit
GGGTGAGTGGGAGAGGGAGATGCAGGCTGTCGAGCTGAGCAGGGAGCCTG260.16526824307144672No Hit
CCCCTAAACTCTCCTTTATACCATGCTCCAGATAGGGTGCTGAGAGAGAG260.16526824307144672No Hit
GATGTATTGGGGAAAAAAAATCACTGTGGGAAAGAAAGCCTGGGTTGTAT260.16526824307144672No Hit
ACATTGAACAGATCATCTTTGCTAACCGTTGGTATTTGGTGGGTGTGTCT260.16526824307144672No Hit
CTGTTGGGAAGTCTGCCTTCTCACTTCACATTATATTGTGGATCTTTTTT250.1589117721840834No Hit
CAATATACCCTTTGACACAGAAATTCTGCTTCTCAGAGCTTACCCTCAGG250.1589117721840834No Hit
GTTCTACATGATCTCACCAACTTAACATGTCCAAAGCAAAATCAGTTCCT250.1589117721840834No Hit
GTACTAGGGCCAGGGTCTCTCTAATGAGAGACCACAGGACACCTTAACCA250.1589117721840834No Hit
AATATGTACAGAGAAATTGACCTTTTAAATCATTCTTTCCTCTAAGCAGA250.1589117721840834No Hit
ATCAAAATGTTTGCCAAACAAAACAAAAAATATTTGCCAGTCTTGGGGCA250.1589117721840834No Hit
CCACAATATAATGTGAAGTGAGAAGGCAGACTTCCCAACAGTGTGCAAAA250.1589117721840834No Hit
GTTTCTGTAGACTGTAGTTTTGAGAACAGAGTTGCTAAGGGTCTCAGAGG250.1589117721840834No Hit
TCTCAGAGCTTACCCTCAGGAAATGAGTGGAACATGCACATGGCTACAGT250.1589117721840834No Hit
GAGTAATACTGCTCCCATCTCCAATTTACAGCCCTGTGAAAGCCGTCAGT240.15255530129672007No Hit
GTCTTGGGGCATCTGGGTGGCTCATTCGGTTAAAAGCATCTGCCTTCAGC240.15255530129672007No Hit
CAATAATAGTTACTGACGGCTTTCACAGGGCTGTAAATTGGAGATGGGAG240.15255530129672007No Hit
CCCTAGTACTGTAAAAGCTTCTACCGACACTACCGCTCTATCCTGCCATC240.15255530129672007No Hit
GTAAAGGACAGTAGTTAAATTTCCATGTCAGATGGAAATGTTTTGAATTT240.15255530129672007No Hit
CTAATTTACTCTCCCACCAGCAATGGGAAAGGATGCCTATTTATTTCATC230.14619883040935672No Hit
GTTATATAAACTTGAGCCCCTTTGCTTTACACTGTTGAAGGGTTTGCATT230.14619883040935672No Hit
GTATACTGCTGTATGGTTGTACCACAGTCTAGCCTGCCTCTTATTGATAG230.14619883040935672No Hit
ATTTACATGAACTCTGACATCTTGCCAAACTCACATTTTTTACACATCAA230.14619883040935672No Hit
GTATTGACCAGAAGTGTGATTTCCTAGCTTTAGGAATTTGTACAGCCCCA230.14619883040935672No Hit
GTACAGAGAAATTGACCTTTTAAATCATTCTTTCCTCTAAGCAGACTCCC230.14619883040935672No Hit
GGCTAGACTGTGGTACAACCATACAGCAGTATACTATGCAGCACTTAAAT230.14619883040935672No Hit
GTACACATACTCAACAAGGTTTTTAAGAATCCACAATGAGGGTTGCTGGG230.14619883040935672No Hit
CTCCTGAGCAGAGAGCCCAATGCAGCCAAAGGCAGACACCTGACTGACTG230.14619883040935672No Hit
GGGAAAGGATGCCTATTTATTTCATCATCCAGTTAACACAGATTTTTCAG220.1398423595219934No Hit
GATTTGAAGAGAGGAGGGAGGAGATGGCAGGATAGAGCGGTAGTGTCGGT220.1398423595219934No Hit
ATTTATAAGAACCTTGTGTATGATGAAACAAGTTTGGAATGAGCTAAACT220.1398423595219934No Hit
GTGGCAGGGAGAGGGAGAACGAGGCTCGTGCTGAGCAGGGAGCATGATGT220.1398423595219934No Hit
GTACTTCACTGGGTCACTATTGCACACGTATTTGGGCCGTGACAGGCAGG220.1398423595219934No Hit
ATTTACAGCCCTGTGAAAGCCGTCAGTAACTATTATTGTGGTTAAGGTGT210.13348588863463007No Hit
GTCCAAGGTAGGATTTAAGAAAATTAAGCTACCAAAAGAAAAACATCAAC210.13348588863463007No Hit
ATCCTGCCATCTCCTCCCTCCTCTCTTCAAATCAGGCCCTCCCCTAAACT210.13348588863463007No Hit
GCAGTATACTATGCAGCACTTAAATGACACAAGGTACACATACTCAACAA210.13348588863463007No Hit
AACTAGAAGAGTGTGATGTTAGAAGTCTAGGAAGAAGTAATTCAGAGAGG210.13348588863463007No Hit
GTGTATGATGAAACAAGTTTGGAATGAGCTAAACTATCAATAAGAGGCAG210.13348588863463007No Hit
ATACTATGCAGCACTTAAATGACACAAGGTACACATACTCAACAAGGTTT210.13348588863463007No Hit
ATGTTAACTTAACCTTCTGAATGCTTCCCCATCTTTAAAATGGGAATAAC210.13348588863463007No Hit
ACCTTGGACAATTCAAGCTTCTGGGAAGTACTTTGGATTGTTGGAATTAC200.12712941774726672No Hit
GGGGGAAGTGGCAGGGAGAGGGAGAACGAGGCTCGTGCTGAGCAGGGAGC200.12712941774726672No Hit
TGTGTACCTTGTGTCATTTAAGTGCTGCATAGTATACTGCTGTATGGTTG200.12712941774726672No Hit
GCTTTAGCCAAGATCACCAATTCCTTCTGTCTTGCTAAACTCAAAGGCCA200.12712941774726672No Hit
GTATTACTTCAGACTGGCAACCATTTGCTCAAATCTTGAACCCAAATATC200.12712941774726672No Hit
GAATGTAACAAAGGGTAGAAGAAATGAAATCAAATTCACCTAGCAACAGA200.12712941774726672No Hit
CCTTAACCACAATAATAGTTACTGACGGCTTTCACAGGGCTGTAAATTGG200.12712941774726672No Hit
GTACCAGGCTATTTATAAGAACCTTGTGTATGATGAAACAAGTTTGGAAT200.12712941774726672No Hit
GTCCTGAGCTGAAGGCAGATGCTTTTAACCGAATGAGCCACCCAGATGCC200.12712941774726672No Hit
GCCTGGTACTGTAGCCATGTGCATGTTCCACTCATTTCCTGAGGGTAAGC200.12712941774726672No Hit
CTCATTAGAGAGACCCTGGCCCTAGTACTGTAAAAGCTTCTACCGACACT190.12077294685990338No Hit
CAATGAGGGTTGCTGGGTAGCTCAATCGTTAAGCGTCTGCCTTCAGCTCA190.12077294685990338No Hit
CATTAGAGAGACCCTGGCCCTAGTACTGTAAAAGCTTCTACCGACACTAC190.12077294685990338No Hit
CTATTGCACACGTATTTGGGCCGTGACAGGCAGGAATGAACTTGTCAAAA190.12077294685990338No Hit
GTTGTAGATCTTCATCCAAAGATGCAGTACTTCACTGGGTCACTATTGCA190.12077294685990338No Hit
ATCAATAAGAGGCAGGCTAGACTGTGGTACAACCATACAGCAGTATACTA190.12077294685990338No Hit
CGAGCAGGGTGAGTGGGAGAGGGAGATGCAGGCTGTCGAGCTGAGCAGGG190.12077294685990338No Hit
AGTTTGGAATGAGCTAAACTATCAATAAGAGGCAGGCTAGACTGTGGTAC190.12077294685990338No Hit
TCCTGCATCCCTGCAAATTTGAAGTCCTGGTTTTCTAGGCAGAAACCATC190.12077294685990338No Hit
TATCTGAAGTGGATGGCTTTAACTGCAGGTAAATTGTGCCTTAAAAAGTA190.12077294685990338No Hit
TCTTTAAAATGGGAATAACGAGGGGCGCCTGGATGGCTGTCAGTCAGTCA190.12077294685990338No Hit
TATTTGGGCCGTGACAGGCAGGAATGAACTTGTCAAAAGTCACCCCTCTG190.12077294685990338No Hit
TTTTAAGCCCATGAAAAGGAATTTCAGAATGAAGTCTGTAATTCCAACAA190.12077294685990338No Hit
CTATTAGATATCTCGACTATTTAGACATTGAACAGATCATCTTTGCTAAC190.12077294685990338No Hit
CTATTATTGTGGTTAAGGTGTCCTGTGGTCTCTCATTAGAGAGACCCTGG180.11441647597254005No Hit
GTCCTGTGGTCTCTCATTAGAGAGACCCTGGCCCTAGTACTGTAAAAGCT180.11441647597254005No Hit
ATCTGATAGTTATTAAGATACGAAATATCTGAAGTGGATGGCTTTAACTG180.11441647597254005No Hit
AGTTAACACAGATTTTTCAGTTTATCAAAATGTTTGCCAAACAAAACAAA180.11441647597254005No Hit
CTTAATAACTATCAGATAAATTGACTATTAGATATCTCGACTATTTAGAC180.11441647597254005No Hit
GGAATAACGAGGGGCGCCTGGATGGCTGTCAGTCAGTCAGGTGTCTGCCT180.11441647597254005No Hit
TCCCAAGACTCCGTCTGGTTACATTACCAAACTCCCCATAGCTTATAAGG180.11441647597254005No Hit
CCCTTTGACACAGAAATTCTGCTTCTCAGAGCTTACCCTCAGGAAATGAG180.11441647597254005No Hit
GGCAAAGACTTAGTAAAGGACAGTAGTTAAATTTCCATGTCAGATGGAAA180.11441647597254005No Hit
CTCCATTGATGTATTGGGGAAAAAAAATCACTGTGGGAAAGAAAGCCTGG180.11441647597254005No Hit
TTATAGGGATGTGCAGTCCTGCCCCCTTTCCTGTCTACATTTTCTGTCCC180.11441647597254005No Hit
GCTCCAGATAGGGTGCTGAGAGAGAGACTGAACCCAGGGAGAGAATGCAA180.11441647597254005No Hit
GTTATTAAGATACGAAATATCTGAAGTGGATGGCTTTAACTGCAGGTAAA170.10806000508517671No Hit
GTATTGGGGAAAAAAAATCACTGTGGGAAAGAAAGCCTGGGTTGTATTGA170.10806000508517671No Hit
TTGTTACATTCTTAAGAATGGAAATCCGTTGTTATTGTAAAATAAATCCC170.10806000508517671No Hit
GAACAGATCATCTTTGCTAACCGTTGGTATTTGGTGGGTGTGTCTGCTAA170.10806000508517671No Hit
TATAAATCCAATATACCCTTTGACACAGAAATTCTGCTTCTCAGAGCTTA170.10806000508517671No Hit
ATTTATTTCCCTAGAGAGGGAACACGAGCAGGGTGAGTGGGAGAGGGAGA170.10806000508517671No Hit
AATAGTTACTGACGGCTTTCACAGGGCTGTAAATTGGAGATGGGAGCAGT170.10806000508517671No Hit
GGCAGGGAGAGGGAGAACGAGGCTCGTGCTGAGCAGGGAGCATGATGTGG170.10806000508517671No Hit
AGATTGAGTATCCTGCATCCCTGCAAATTTGAAGTCCTGGTTTTCTAGGC170.10806000508517671No Hit
TTGCTGGGTAGCTCAATCGTTAAGCGTCTGCCTTCAGCTCAGGTCATTTC170.10806000508517671No Hit
CTTATACATAGCATAAATATTTCATACTGGACTGAAATAATCACGAGTAC160.10170353419781336No Hit
CTTATAAATAGCCTGGTACTGTAGCCATGTGCATGTTCCACTCATTTCCT160.10170353419781336No Hit
GTTAAAGCCATCCACTTCAGATATTTCGTATCTTAATAACTATCAGATAA160.10170353419781336No Hit
CCGTTGGTATTTGGTGGGTGTGTCTGCTAAGTTGGCTGTCAGATCCCTTT160.10170353419781336No Hit
ATATAACAGCCCACAAATATTTTTCAAAACATGGCAAAGGGATCTGACAG160.10170353419781336No Hit
CCCACCAGCAATGGGAAAGGATGCCTATTTATTTCATCATCCAGTTAACA160.10170353419781336No Hit
CTAGAAGAGTGTGATGTTAGAAGTCTAGGAAGAAGTAATTCAGAGAGGGA160.10170353419781336No Hit
GTTTAGCTCATTCCAAACTTGTTTCATCATACACAAGGTTCTTATAAATA160.10170353419781336No Hit
GTCCAAAGCAAAATCAGTTCCTTCCCCAGTCTTTGCCAGCCCATAAATGG160.10170353419781336No Hit
TTTCAAAACATGGCAAAGGGATCTGACAGCCAACTTAGCAGACACACCCA160.10170353419781336No Hit
GTGCATGTTCCACTCATTTCCTGAGGGTAAGCTCTGAGAAGCAGAATTTC160.10170353419781336No Hit
TTACAGCCCTGTGAAAGCCGTCAGTAACTATTATTGTGGTTAAGGTGTCC160.10170353419781336No Hit
GGCTTTAACTGCAGGTAAATTGTGCCTTAAAAAGTAGATAAAGGAGAAAA160.10170353419781336No Hit
ATCTAGGATATAGTTTCTAGGGGGAAACAATACCACTCAGATTGCATTGA160.10170353419781336No Hit
GTGGTTACCCTGGGTAAGGATGGTTTCTGCCTAGAAAACCAGGACTTCAA160.10170353419781336No Hit
CTACAGAAACTGGGCTGAAATTGCCTAGATTGAGTATCCTGCATCCCTGC160.10170353419781336No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG151.2072509E-4145.02
CGAAAGC151.2072509E-4145.03
AGCGAAA151.2072509E-4145.01
GAAAGCA151.2072509E-4145.04
CAGGGGG203.7947978E-4108.759
AAAGCAG203.7947978E-4108.755
AAGCAGG203.7947978E-4108.756
AGGGGAG502.2021231E-672.55
GTTTAGG502.2021231E-672.51
TTTAGGG502.2021231E-672.52
TTAGGGG502.2021231E-672.53
TAGGGGA502.2021231E-672.54
GGGGAGG502.2021231E-672.56
GGGAGGG553.878664E-665.9090967
GAGGGCC553.878664E-665.9090969
CTAGAGA451.15776944E-464.444444
CCTAGAG451.15776944E-464.444443
TCCCTAG451.15776944E-464.444441
GGAGGGC606.499773E-660.4166688
TAAGACA400.00594098154.3754
Produced by FastQC (version 0.11.2)