FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2435_day_14_nw.35200000088656.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2435_day_14_nw.35200000088656.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107893
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3290.30493173792553735No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2670.24746739825567926No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1210.11214814677504566No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT1170.10844077002215156No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTAC100.0070821554145.01
CGTAGTG203.8566088E-4108.755
GCGTAGT203.8566088E-4108.754
AGCGTAG259.3638926E-487.03
AAGCGTA300.001931046572.52
AGCGAAA1250.063.8000031
GAGCAAA3000.060.4166681
TACCCTA502.0021765E-458.05
AAAGCGT400.00603631654.3751
GAGCGAA1152.4483597E-950.4347841
CAAAAGC3650.049.6575364
GCGAAAG1650.048.3333362
GCAAAAG3850.047.0779233
CGAAAGC1551.4551915E-1146.7741933
CAGCTCG1652.7284841E-1143.9393969
AGCAAAA4350.041.6666682
ATACAGC2252.0008883E-1135.4444476
TATACAG2302.5465852E-1134.6739165
AAAAGCA5300.034.1981125
TATACAT2353.274181E-1133.9361724
Produced by FastQC (version 0.11.2)