FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2435_day_14_ll-e.3520000008883a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2435_day_14_ll-e.3520000008883a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163921
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA9720.5929685641254018No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC7060.4306952739429359No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA3950.240969735421331No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA3350.20436673763581237No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2590.1580029404408221No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA2580.15739289047773014No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2510.1531225407360863No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2120.1293305921754992No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1940.11834969283984359No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1900.11590949298747567No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1870.11407934309819974No Hit
GTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCCCTACTATTCC1870.11407934309819974No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1850.1128592431720158No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1840.1122491932089238No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1800.1098089933565559No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1790.10919894339346393No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1790.10919894339346393No Hit
GTATACATACAATGGCATATTACTTGGTCTTCAGATGGATGGAAATCCTG1710.1043185436887281No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1670.1018783438363602No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1650.10065824391017623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1550.098.225811
GCAAAAG9950.088.1658253
AGCAAAA10150.086.428572
CAAAAGC10450.083.9473654
GAGCAAA10950.080.776251
TAGGTCC300.00193284672.59
AAAGCAG14400.070.4861156
AAAAGCA13000.067.480775
GCGAAAG2150.064.069773
AGCGAAA2150.064.069772
ACCCGTT350.003561186462.142857145
CGAAAGC2300.059.8913084
AAGCAGG17550.058.2478647
GCAGGAT805.8433034E-754.3750047
GCAGGGG5050.048.811889
GTAGAAA2550.048.3333321
AGCAGGG13750.044.290918
GTAAGGG700.001054054841.428573
AGCAGGA4400.037.8977248
GCAGGAG4550.033.4615369
Produced by FastQC (version 0.11.2)