FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2433_day_14_nw.352000000885c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2433_day_14_nw.352000000885c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101255
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1760.17381857686040195No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1560.1540664658535381No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACAC300.001930703872.53
AGCGAAA902.7830538E-1064.444451
GAGCGAA606.76166E-660.4166681
CGAAAGC1102.5465852E-1159.3181843
GCGAAAG1051.0913936E-955.2380942
TTTAGAC400.00603525354.3753
ATTAGAG907.434824E-540.277783
TATACAG1303.654677E-739.0384645
CAAAAGC2302.5465852E-1134.6739164
CAGCTCG1051.842005E-434.5238089
GAGCAAA2051.1174052E-831.8292681
CAAGACT950.004707468730.5263184
AGCAAAA2651.3460522E-1030.0943412
CCAAGAC1000.006049053329.03
GCAAAAG2802.564775E-1028.4821433
ATCTAAT1100.00963137626.3636381
TCTAATA1100.00963137626.3636382
TATATAT2704.3800283E-621.481485
AAGCAGG5800.021.257
TGGGAAG2455.0560287E-520.7142855
Produced by FastQC (version 0.11.2)