FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2433_day_14_ll-e.35200000088a38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2433_day_14_ll-e.35200000088a38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165474
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA12780.7723267703687589No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC10490.6339364492306949No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA6320.38193311335919844No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA6020.3638033769655656No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA5120.30941416778466707No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC3960.23931252039595344No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA3690.2229957576416839No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA3660.22118278400232058No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA3450.20849196852677762No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT3190.1927795303189625No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA2820.17041952210014866No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2760.1667935748214221No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2590.15652005753169682No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC2560.15470708389233354No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT2470.1492681629742437No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA2450.14805951388133484No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG2430.146850864788426No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2430.146850864788426No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2410.14564221569551714No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA2350.14201626841679055No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC2340.14141194387033612No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2270.13718167204515513No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG2270.13718167204515513No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC2180.13174275112706527No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA2150.12992977748770201No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA2030.12267788293024887No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA2010.12146923383734001No Hit
AGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1990.12026058474443116No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1980.11965626019797672No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1950.11784328655861344No Hit
GGTTAATCTATTTTTATTAAATATATTTATTGTCATGGTTGGGCTTGAGT1930.11663463746570459No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1910.11542598837279573No Hit
GAGCCGAAGGCAGAGGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAAT1890.11421733927988688No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1890.11421733927988688No Hit
AGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACC1780.10756976926888816No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1780.10756976926888816No Hit
CCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGA1770.10696544472243374No Hit
AGACTAAACACTGCAGTTAAAGTCAGGGATTTGGGGGCACCTGGGTGGCT1770.10696544472243374No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT1740.10515247108307045No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1740.10515247108307045No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC1730.10454814653661604No Hit
AGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACA1700.10273517289725274No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAAAA9300.085.752692
GAGCGAA1850.082.29731
GCAAAAG10000.079.753
CGGTTTT300.001932878372.5145
AAAAGCA11100.072.55
CAAAAGC11550.069.675324
AAAGCAG13900.069.370516
GAGCAAA11900.068.8445361
GCAGGAG3550.063.309869
CGAAAGC2700.061.7592623
GCGAAAG2850.058.508772
AGCGAAA2950.056.525421
GTACAAG400.006042000454.3749961
ACTGTGG400.006042000454.3749968
GCAGGTA400.006042000454.3749969
GAAAGCA3400.053.3088234
AAGCAGG19650.048.7022867
ACCATCG450.009625033548.3333328
GTGTAAA604.933044E-448.3333321
ACAAGGT752.541285E-548.3333327
Produced by FastQC (version 0.11.2)