FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2432_day_14_nw.352000000886a6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2432_day_14_nw.352000000886a6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98785
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3120.3158374247102293No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2090.21157058257832667No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1950.1973983904438933No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT1680.17006630561320038No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA1180.11945133370450979No Hit
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1110.1123652376372931No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1060.10730374044642405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA3100.095.887091
GAGCGAA502.2473614E-887.01
AGCAAAA4200.069.0476152
GCAAAAG4500.062.8333363
CAAAAGC4750.059.526314
AGCGAAA1007.075869E-1058.01
TTAGTCT400.00603481354.375145
CTATACT553.2047922E-452.7272764
TAGATAG553.2047922E-452.7272765
GGTTAAT450.00961362748.3333321
AAAAGCA6000.047.1255
GCGAAAG1255.114998E-946.3999982
AAAGCAG7200.045.31256
CGAAAGC1401.3924364E-841.428573
TACATAC1303.6534402E-739.0384646
TACTTAC1553.416426E-837.4193537
GCAGGCC1051.8416761E-434.5238089
TATACAT1905.322363E-934.3421024
TATAACA850.002730091634.117652
CATACAT1501.1202173E-633.8333368
Produced by FastQC (version 0.11.2)