FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2431_day_8_nw.352000000886f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2431_day_8_nw.352000000886f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57421
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1750.3047665488236011No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1370.2385886696504763No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1020.17763535988575607No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT870.15151251284373313No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA620.10797443444036153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGGT100.007073584144.99998145
CCGTGAT100.007073584144.99998145
GAGCAAA1850.078.378381
AGCGAAA607.8973244E-872.52
TAGGCCC300.001926427172.54
AGCAAAA2100.069.0476152
GCAAAAG2100.069.0476153
GCGAAAG651.3747194E-766.923073
GAGCGAA651.3747194E-766.923071
GTACAAT350.003549405462.1428533
CAAAAGC2500.058.04
CGAAAGC805.7762736E-754.3749964
GAATGAG400.00602198554.3749963
CACATAC450.00959326748.3333326
GTACTAT450.00959326748.3333326
GCTGTAA450.00959326748.3333321
TGTACAA450.00959326748.3333322
ATGTTAC450.00959326748.3333321
AAAGCAG3950.047.721526
AAAAGCA3150.046.0317465
Produced by FastQC (version 0.11.2)