FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2431_day_8_ll-e.352000000887ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2431_day_8_ll-e.352000000887ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72526
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA4730.6521799078950997No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC3240.44673634282877867No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2130.2936877809337341No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2060.2840360698232358No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT1950.2688690952210242No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1670.23026225077903095No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1470.2026859333204644No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1340.1847613269723961No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1150.15856382538675787No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1110.15304856189504454No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1110.15304856189504454No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1020.14063921903868956No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA1010.13926040316576124No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA980.13512395554697626No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA960.1323663238011196No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC920.12685106030940627No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA910.12547224443647795No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC890.12271461269062128No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT890.12271461269062128No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA880.12133579681769296No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA880.12133579681769296No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA830.1144417174530513No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC830.1144417174530513No Hit
GAGCAAAAGCAGGGGATTAGGAGTACACTTTCTGTGAGAAGCTCTGAGTA810.11168408570719467No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC810.11168408570719467No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT800.11030526983426633No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG790.108926453961338No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA770.10616882221548135No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT750.10341119046962469No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA750.10341119046962469No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTC100.0070773996145.07
GGTCTCA100.0070773996145.07
GAGCGAA1000.0108.751
AGCAAAA3900.087.3717962
GCAAAAG4300.079.244193
CAAAAGC4450.074.9438254
ACAAGGT300.001928483472.53
AAAGCAG6350.070.78746
GCGAAAG1650.070.303033
AGCGAAA1650.070.303032
AAAAGCA4750.070.2105265
CGAAAGC1700.068.235294
GAGCAAA5000.068.151
GCAGGGG1750.066.285719
TCTTACC350.003553178962.1428577
AACATCG350.003553178962.142857145
AGTACAC702.3042048E-762.1428573
AAGCAGG7500.061.866667
GGAGTAC805.7977013E-754.3751
AGCAGGG5350.051.4953238
Produced by FastQC (version 0.11.2)