FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2430_day_8_ul-b.352000000887db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2430_day_8_ul-b.352000000887db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64982
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1680.25853313225200825No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1640.2523775814841033No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1550.23852759225631714No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1530.23544981687236466No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA1370.21082761380074483No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT1300.20005539995691116No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1230.18928318611307748No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA1200.18466652303714876No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1200.18466652303714876No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1130.17389430919331508No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA970.14927210612169522No Hit
GTCTAGTTAATAAAGCCCTACTATTCCACCGTGCTAACAGTCCCTTTGAG960.14773321842971898No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC950.14619433073774277No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT920.14157766766181404No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG900.13849989227786155No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA870.13388322920193285No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA830.12772767843402788No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT760.11695546459019421No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA750.11541657689821798No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT740.11387768920624172No Hit
ATCTAGTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCC710.10926102613031301No Hit
ATGTATGAGTTACTAATTTCCTGGACATTATCAGCCATTAGCTGGTTTCC700.10772213843833678No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG690.10618325074636054No Hit
GCATTAAGATGTATGAGGTGTATTCCTTCCCCATCTTGGGAATTCTAGTT680.10464436305438428No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA680.10464436305438428No Hit
GTTAATATACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTA670.10310547536240805No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG660.10156658767043181No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC660.10156658767043181No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAC100.0070757144144.99998145
GAGCGAA351.873559E-9124.285711
AGCGAAA1050.096.6666641
GCAAAAG3350.093.05973
CGAAAGC1250.087.03
AAAAGCA3600.086.597225
CAAAAGC3650.085.4109654
GCGAAAG1300.083.653852
AGCAAAA4250.073.3529362
GATTAGC300.001927574772.58
AAAGCAG5100.071.078446
GAGCAAA4700.067.8723451
AGGATTA350.003551511362.1428576
GAAAGCA1850.058.783784
ATTAGCA400.00602554554.3749969
AAGAGGG400.00602554554.3749965
GCAGGTA400.00602554554.3749969
TTTCTAC400.00602554554.3749963
ATCCTAT450.00959891548.3333321
AGGTATT450.00959891548.3333324
Produced by FastQC (version 0.11.2)