FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2430_day_8_ll-e.35200000088a88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2430_day_8_ll-e.35200000088a88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133278
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA5290.39691471960863756No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC4700.35264634823451735No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA4190.3143804678941761No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA4040.30312579720584043No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA3740.2806164558291691No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT3290.24685244376416215No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG2760.20708594066537614No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA2680.2010834496315971No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC2650.19883251549392997No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT2490.18682753342637196No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC2460.18457659928870482No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA2410.18082504239259292No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT2360.17707348549648105No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT2270.17032068308347964No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA2190.1643181920497006No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA2150.16131694653281112No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA2070.1553144554990321No Hit
GTATGTTAATTACACTGGAATTAAAAACAAAGATATGCATCTTGGAGACA2070.1553144554990321No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT2000.15006227584447546No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1880.14105853929380693No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1860.13955791653536218No Hit
TTATAAAAGAAGTCAAAGGCGGTCTTGAATAAGTGGAAGGCTTCTGCCAG1750.13130449136391603No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1730.12980386860547127No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1690.12680262308858176No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1670.125302000330137No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1610.12080013205480274No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA1600.12004982067558036No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1600.12004982067558036No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT1570.11779888653791323No Hit
GTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGGAATCCAGAAG1540.11554795240024611No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1510.11329701826257897No Hit
GAGCAAAAGCAGGGGATTAGGAGTACACTTTCTGTGAGAAGCTCTGAGTA1500.1125467068833566No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT1490.11179639550413423No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1460.10954546136646708No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1460.10954546136646708No Hit
AGATTAACCAGGGCAACTAGGTGGCTCAGTCGATTGTCTGCCTTCACCTC1430.10729452722879995No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1420.10654421584957757No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC1420.10654421584957757No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1400.10504359309113283No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCCT100.0070840167144.999986
TTCGAGA100.0070840167144.999988
GAGCGAA1350.0107.407411
AGCAAAA7100.081.690142
AGCGAAA2550.079.607851
GCAAAAG7300.079.452063
GCGAAAG2650.076.6037752
GTTTCGT300.001932049872.58
TCTCGGG502.2952718E-672.5145
AAAAGCA8100.071.6049355
CGAAAGC2800.069.910713
GCAGGTA451.1900788E-464.444448
CAAAAGC9150.063.3879784
TGTTTCG350.003559724762.1428577
TTTCGTT350.003559724762.1428579
TATGCTA350.003559724762.1428572
AAAGCAG12000.059.8124966
GAGCAAA10500.059.3809551
AGCACAC400.00603942954.3749963
TTAGCAC400.00603942954.3749961
Produced by FastQC (version 0.11.2)