FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2429_day_8_nw.3520000008858d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2429_day_8_nw.3520000008858d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80946
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG1800.22237046920169004No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1650.20383959676821584No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1600.19766263929039113No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1380.17048402638796234No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC1280.1581301114323129No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1240.15318854545005312No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1090.13465767301657897No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1020.12600993254762433No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG1020.12600993254762433No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG950.11736219207866973No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT890.10994984310528007No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAC100.007078906145.02
GAGCAAA2850.0101.754391
GCAAAAG3150.087.460323
TCCGTGT259.355367E-487.0145
AGCAAAA3250.084.769232
CAAAAGC3450.079.855074
CTATGTA300.001929295472.59
AAAAGCA4350.063.3333365
GAGCGAA953.0913725E-853.4210471
GCGAAAG1101.6425474E-952.7272763
TAGGTTA701.6820484E-551.7857136
GGTTATC701.6820484E-551.7857138
GTTATCA701.6820484E-551.7857139
AGCGAAA1152.4356268E-950.4347842
GTATAGG752.5296735E-548.3333363
CGAAAGC1203.5506673E-948.3333324
TTATAGC450.0096073948.3333324
AAAGCAG6750.048.3333326
ATAGGTT803.70411E-545.31255
AGGTTAT855.297914E-542.6470577
Produced by FastQC (version 0.11.2)