FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2429_day_8_ll-e.3520000008878b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2429_day_8_ll-e.3520000008878b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100199
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA7470.745516422319584No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC5420.5409235621113984No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA2720.2714597950079342No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA2690.26846575315122906No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2390.2385253345841775No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT2220.2215590973961816No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC1910.19062066487689497No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1890.18862463697242488No Hit
GCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACA1780.1776464834978393No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA1690.16866435792772383No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA1530.152696134691963No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1470.1467080509785527No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC1320.1317378416950269No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA1320.1317378416950269No Hit
GTATATATGAAACTAATATCACACTGTATGTTAATTACACTGGAATTAAA1270.12674777193385164No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA1250.12475174402938154No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA1250.12475174402938154No Hit
GAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC1240.12375373007714648No Hit
GAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1230.12275571612491143No Hit
GAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCG1210.12075968822044132No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1180.11776564636373615No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT1130.1127755766025609No Hit
GAGCAAAAGCAGGCAAGGGCCCCGAGCCATGGAAAACAGTCGACAGCCCA1130.1127755766025609No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC1120.11177756265032585No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG1110.1107795486980908No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA1100.10978153474585574No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1100.10978153474585574No Hit
CTTATTATGAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGA1090.10878352079362069No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT1060.10578947893691554No Hit
TATTTATTGTCATGGTTGGGCTTGAGTTCATTTTTTTTCTGGTGAATGGC1050.10479146498468049No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG1050.10479146498468049No Hit
AGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGAC1020.10179742312797532No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCCC100.0070814057145.07
GAGCGAA1650.0101.060611
AGCAAAA4550.086.043962
GCAAAAG4550.086.043963
GCGAAAG1900.083.947373
CGAAAGC1950.081.7948764
CAAAAGC5000.078.2999954
AGCGAAA2050.077.804882
AAAGCAG7250.077.06
TTACCCC300.001930642272.59
CTTACCC300.001930642272.58
TCTATAC554.0350005E-665.9090963
GTAGAAA1150.063.043482
GCAGGTT350.003557142362.1428579
GAGCAAA6450.060.6976741
AAAAGCA6650.059.9624065
TAGTTAA502.0014825E-458.04
AAGCAGG10000.055.8257
CTATACC400.00603506254.3754
AGCAGGT953.101377E-853.4210558
Produced by FastQC (version 0.11.2)