FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2428_day_8_nw.3520000008853d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2428_day_8_nw.3520000008853d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109335
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4350.39785978872273287No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3380.30914162893858327No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2860.2615813783326474No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2010.18383866099602142No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1570.14359537202176795No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC1350.12347372753464124No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1330.12164448712672063No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1300.11890062651483972No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG1240.11341290529107788No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1240.11341290529107788No Hit
CCCCAACTGCATTTTTGACATCCTCATAAGTATGTCCTGGAAGAGAAGGT1180.10792518406731605No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT1170.10701056386335575No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTT203.8567497E-4108.759
GAGCAAA3750.092.81
GAGCGAA1250.087.01
AGCGAAA1500.067.666672
GCAAAAG5200.066.923083
AGCAAAA5200.065.528852
GTCTATC350.00355801262.1428572
CGAAAGC1700.059.705884
CAAAAGC6250.058.04
GGATAGG400.006036533554.3751
ATCCCGA400.006036533554.3756
AGCAGGT2000.054.3758
CCCGACC400.006036533554.3758
AAAGCAG8650.053.641626
GCGAAAG1950.052.0512853
AAAAGCA7100.051.056345
GCAGGGT3050.049.9180349
TGACAGG604.925645E-448.3333323
TATCCCG450.00961635648.3333325
AAGCAGG10400.044.6153877
Produced by FastQC (version 0.11.2)