FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2428_day_8_ll-e.3520000008892c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2428_day_8_ll-e.3520000008892c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56619
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2660.46980695526236776No Hit
GAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA2500.4415478902841803No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1880.3320440134937035No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA1780.31438209788233634No Hit
GCTAAACAAGGACCCTGATTCGGGGCTTGATCCCAGGACTTTGGAATCAT1620.28612303290414876No Hit
GAGCAAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAAT1500.26492873417050816No Hit
GGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAATATGAA1410.24903301012027765No Hit
GTATATAAGCATGCAAATCCATGAATAGCTGAACCAGCAGAGGCCAATTC1400.24726681855914093No Hit
GGTCTAACCCACTGAGCCATCCAGGTGCCCCCAAATGTTCATTTTTTAAT1360.24020205231459404No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1360.24020205231459404No Hit
ATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCTTAG1290.227838711386637No Hit
GTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCTTT1050.1854501139193557No Hit
CTTAACAACTGAGCCACCCAGGTGCCCCCAAATCCCTGACTTTAACTGCA960.16955438986912522No Hit
CTATGGAATGCCGCTCACTGTAAGCTTTGAACTACTGACTTGGACATCAT940.16602200674685175No Hit
GTAGATACAGAGAAATAAAGATAAAAATTACACTGGCCATTCACCAGAAA910.1607234320634416No Hit
AGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCAC910.1607234320634416No Hit
ATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTCATGT860.151892474257758No Hit
GCTCTAAGAAGCTCAATGAATTCCACTAAGGGTAAATATAAAAAAACGAC840.14836009113548457No Hit
GAATATAACTTCTAGGACTAAAAAAATACAATATCTGAATTGGAAAAGCC810.14306151645207438No Hit
AGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATA800.14129532489093766No Hit
GGATGAGAGACAGATTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAG790.13952913332980096No Hit
GATACATATCACACTTGAATTGCTGAAAATAATAATAAAGAAAATCCATG780.13776294176866422No Hit
GGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGACAAATAGGGAA770.13599675020752752No Hit
GAGCAAAAGCAGGCACATACCGAAATACTTAATTTCTGTCTGATTTTTTT770.13599675020752752No Hit
ATATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGA740.13069817552411733No Hit
GCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAA720.12716579240184392No Hit
CCTTAGAGACAAGCTAATTTGCCAGATAGTAATATTTAAAATGTAGGTAA720.12716579240184392No Hit
GCCTTAACAGAGACCATAAAAGAAAAGCTCAAGGCACTTGAAGACAGAAA710.12539960084070717No Hit
ATGTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGC700.12363340927957046No Hit
GAATAAGGATAAGAATATAAAAAATACGACCACAATAGGAGAACCAAAAA680.12010102615729702No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA670.1183348345961603No Hit
GGTTAAGAGTAAATTACTAAGGAAAAAACTACTCTCAAGTTATCAAAGGT660.1165686430350236No Hit
CCTTTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAA660.1165686430350236No Hit
GTGGGAGAGAGGGAGGCACGCTTCCTGCTAAACAAGGACCCTGATTCGGG650.11480245147388686No Hit
GAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCA650.11480245147388686No Hit
GTATCAGAGTCGTTTTTTTATATTTACCCTTAGTGGAATTCATTGAGCTT640.11303625991275013No Hit
CCCCACTCAGCAGGGAGCCCAATGTGGGACTCGATCCCAGGACCCTGAGA630.11127006835161342No Hit
GTCTATCTTGGGCTAGAGATAGAATCATGAAAGTGCTTTAGAATTCAGTC630.11127006835161342No Hit
CTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACATCATGGATTTTCT630.11127006835161342No Hit
TTTCTGTTCCTTACGCCTTTAGAATAGTGATATTAGGGTGACACGTGCCG620.10950387679047668No Hit
ACTTAATACCATTGAATTATACATAGACAAGTGGTTGGATTATATGGTAC620.10950387679047668No Hit
GTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCCT610.10773768522933998No Hit
GCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCTCCCCACT600.10597149366820326No Hit
TTAGAATAGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATT600.10597149366820326No Hit
GATGTATGTAGTATGATTCCACTTATATAAAAACAAACAAAAAAAGGAAA580.10243911054592982No Hit
AGTGATATTAGGGTGACACGTGCCGTTCTGCATTTTTAAAATTAAATTCC580.10243911054592982No Hit
TTAGTGATCTGTGGAATAACATCAAGAGACCTATCATTTGGTATAACTGG570.10067291898479308No Hit
GAATTATACATAGACAAGTGGTTGGATTATATGGTACATGAATTGTATCA570.10067291898479308No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGG100.007073325145.08
TATGGTG100.007073325145.05
CTACGTC100.007073325145.04
GCAGGTA100.007073325145.09
TAATCGT100.007073325145.0145
GAGCGAA404.7330104E-9108.751
AGCGAAA950.091.578941
AAAAGCA3600.088.6111155
AGCAAAA3700.088.175672
ACAAGGT259.340732E-487.03
GCAAAAG3750.086.999993
CGAAAGC1050.082.857143
GCGAAAG1050.082.857142
GAGCAAA3900.081.7948761
AAAGCAG5050.071.782176
CAAAAGC4750.067.15794
GCAGGGG1850.062.7026989
GAAAGCA1400.062.1428534
AGCAGGT702.2950735E-762.1428538
GTAGAAA753.6977508E-758.0000041
Produced by FastQC (version 0.11.2)