FastQCFastQC Report
Wed 12 Apr 2017
000000000-ATLLR_l01n02_2427_day_5_ll-e.3520000008882d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-ATLLR_l01n02_2427_day_5_ll-e.3520000008882d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131706
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG5100.3872260944831671No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4150.3150957435500281No Hit
GAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAAAGTGA4000.3037067407711114No Hit
GAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGCTAAAC2510.1905759798338724No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2130.16172383946061683No Hit
CAATAGGAGAACCAAAAATATGAAAGAACTGAATATAACTTCTAGGACTA1680.1275568311238668No Hit
TCTTAGAGCTATTGATGTGTTTTTTCATCTAATTTGGAAAATTTTAGCAA1530.11616782834495012No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT1340.10174175815832233No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1330.10098249130639456No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1250.0116.000011
GCAGGTA451.0842996E-896.6666649
CGAAAGC1700.081.029414
AGCGAAA1700.081.029412
GAGCAAA6750.079.4814761
GCGAAAG1750.078.714293
AGCAAAA7300.073.493152
GCAAAAG7650.070.1307143
CAAAAGC8000.067.06254
AAAGCAG10250.066.487816
TAGACTC350.003559633262.1428575
TAAGACT350.003559633262.1428574
GCAGGGT2100.058.6904759
AAAAGCA9550.056.9371725
AAGCAGG12100.056.322317
GCAGGGG3050.054.6721349
GTGTTAA400.006039272554.3751
AGCAGGT1700.051.176478
TCTACGC450.00962070548.333332145
TACTATG2400.048.3333322
Produced by FastQC (version 0.11.2)