000000000-ATLLR_l01n02_2424_day_5_ul-b.352000000889cc.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	000000000-ATLLR_l01n02_2424_day_5_ul-b.352000000889cc.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85297
Sequences flagged as poor quality	0
Sequence length	151
%GC	44

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG	903	1.058653879972332	No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA	467	0.547498739697762	No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG	278	0.32592002063378545	No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT	240	0.28136980198600187	No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC	224	0.26261181518693505	No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT	203	0.23799195751315988	No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC	198	0.23213008663845155	No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC	192	0.2250958415888015	No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG	190	0.22275109323891812	No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG	182	0.2133720998393847	No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA	181	0.21219972566444306	No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG	158	0.18523511964078457	No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA	156	0.1828903712909012	No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT	142	0.16647713284171775	No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG	135	0.15827051361712605	No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC	130	0.15240864274241767	No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT	125	0.14654677186770929	No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT	122	0.14302964934288426	No Hit
GTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTCCTCCG	121	0.1418572751679426	No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT	119	0.13951252681805923	No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC	114	0.13365065594335088	No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT	112	0.13130590759346752	No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA	111	0.13013353341852585	No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT	110	0.1289611592435842	No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC	107	0.12544403671875917	No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC	105	0.1230992883688758	No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA	103	0.12075454001899245	No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT	100	0.11723741749416744	No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG	94	0.11020317244451738	No Hit
ACATAATGGACTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTT	93	0.10903079826957572	No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT	91	0.10668604991969236	No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA	91	0.10668604991969236	No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC	91	0.10668604991969236	No Hit
GAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA	89	0.10434130156980902	No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT	89	0.10434130156980902	No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC	88	0.10316892739486735	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCAGG	10	0.007079571	144.99998	7
AACGCAG	10	0.007079571	144.99998	6
CGCAGGG	10	0.007079571	144.99998	8
GAGCGAA	55	0.0	131.81819	1
GCAGGGT	235	0.0	95.6383	9
GAGCAAA	380	0.0	93.48685	1
AAGCAGG	535	0.0	75.88785	7
AGCAAAA	470	0.0	74.04256	2
AGCAGGG	375	0.0	73.46666	8
TAGAAAC	90	3.6379788E-12	72.5	2
GTAGAAA	90	3.6379788E-12	72.5	1
AAACACT	30	0.0019296525	72.5	5
AGCGAAA	90	3.6379788E-12	72.5	2
ATTATAA	30	0.0019296525	72.5	145
AACACTG	30	0.0019296525	72.5	6
GCAGGTA	135	0.0	69.81482	9
GCAAAAG	510	0.0	68.2353	3
CGAAAGC	100	9.094947E-12	65.25	3
GCGAAAG	100	9.094947E-12	65.25	2
ACACTGC	35	0.0035553256	62.142857	7

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content